Initiation of spontaneous tumors in radish (Raphanus sativus): Cellular, molecular and physiological events

Lebedeva, Maria A.; Tvorogova, Varvara E.; Vinogradova, A. P.; Gancheva, Maria S.; Azarakhsh, Mahboobeh; Ilina, Elena L.; Demchenko, Kirill N.; Додуева, И. Е.; Лутова, Л. А.

doi:10.1016/j.jplph.2014.07.030

Cited by 20 publications

(27 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The WOX5 gene is the central regulator of root apical meri-stem (Sarkar et al, 2007), and according to our data, it also regulates the development of symbiotic nodules in legumes (Osipova et al, 2012). Local analysis of the expression of these genes in the crop-roots and spontaneous tumors of radish lines using reporter constructs carrying the glucuronidase (GUS) showed different spatial expression patterns (Lebedeva et al, 2015;Lutova et al, 2015). Before the initiation of tumors, the expression of both genes takes place in the cambium -in the case of WOX5, it is confined to the part of cambium opposite the proto-xylem pole.…”

Section: Geneticssupporting

confidence: 56%

“…So, it is possible to simulate spontaneous tumor formation in radish in the in vitro system, and this approach greatly simplified work with radish tumors (Buzovkina et al, 1993b). These cytokinin-induced tumors are also anatomically similar to the tumors formed on the crop-roots of tumorous radish lines in the field and are formed as a result of extensive cell proliferation in the lateral meristems -the pericycle and cambium (Ilyina et al, 2006;Lebedeva et al, 2015) (Fig. 2).…”

Section: In Vitro Regenerative Capacity On the Example Of Radish Inbrmentioning

confidence: 76%

“…Externally and anatomically, they are similar to tumors induced by Agrobacterium tumefaciens, and are also capable of hormone-independent growth when isolated from the parent plant. According to histological analysis (Ilina et al, 2006;Lebedeva et al, 2015), these tumors are composed of a mass of small undifferentiated cells with separate foci of meristematic activity and a large number of abnormally oriented vessels. Upon detection of cell proliferation in the roots and tumors of inbred radish lines using the 5'-ethynyl-2-deoxyuridine intercalating dye (EdU), it was shown that during the formation of a spontaneous tumor, the proliferation of a group of interstitial cambium cells and the adjacent xylem parenchymal ray cell takes place, leading to the formation of an outgrowth consisting of undifferentiated actively dividing cells (Fig.…”

Section: Geneticsmentioning

confidence: 99%

“…At later stages, the conductive system of the tumor is formed, eventually mature spontaneous tumors contain a large number of abnormally oriented vessels connected to the root vascular system, to which few proliferating small cells with dense cytoplasm and thin cell walls adjoining cambium cells are attached. The bulk of proliferating cells is concentrated on the periphery of the tumor, at the points where the vascular bundles terminate, and has the appearance of organized structures morphologically similar to the apical meristems (Lebedeva et al, 2015), and the expression of certain meristem-specific genes is concentrated in these meristem-like zones (see below).…”

Section: Geneticsmentioning

confidence: 99%

“…The genes KNAT1 from the KNOX family and WOX5 belonging to the WOX family are meristem-specific genes whose expression is detected in spontaneous tumors in radish lines (Lutova et al, 2008;Tvorogova et al, 2013;Lebedeva et al, 2015). According to the literature, homologs of KNAT1 are regulators of the shoot apical meristem (Reiser et al, 2000), cambium (Liebsch et al, 2014) and nodules (Azarakhsh et al, 2015).…”

Section: Geneticsmentioning

confidence: 99%

See 4 more Smart Citations

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

Lutova

Додуева

2019

BioComm

View full text Add to dashboard Cite

This review highlights the years of research on the genetics of in vitro regeneration in higher plants conducted at the Department of Genetics and Biotechnology of Saint Petersburg State University. The genetic collection of radish (Raphanus sativus) created at the department by selfing of individual plants from three cultivars was used as a model in these studies. Some radish inbred lines from the genetic collection form spontaneous tumors in the roots and are also used to study mechanisms of tumor growth in higher plants. It was revealed that radish lines that differed in the ability to form tumors also contrastingly differed in the reaction of their explants to auxin and cytokinin in vitro, which reflects a difference in the levels of these hormones in the tissues of related tumorous and non-tumorous radish lines. Moreover, high concentrations of cytokinins in cultural medium induced tumor formation in the regenerated plants of tumorous radish lines. The presence of meristematic zones in spontaneous tumors in radish lines, as well as in crown gall tumors induced by Agrobacterium tumefaciens and cytokinin-induced tumors made it possible to reveal the role of the main meristem regulators, such as KNOX and WOX family transcription factors and the CLAVATA system, in both the process of tumor growth and regeneration in plants. Analysis of the expression of meristem-specific genes during the development of spontaneous and induced tumors in radish as well as in regenerated radish plants confirmed this assumption.

show abstract

Section: Geneticssupporting

confidence: 56%

Section: In Vitro Regenerative Capacity On the Example Of Radish Inbrmentioning

confidence: 76%

Section: Geneticsmentioning

confidence: 99%

Section: Geneticsmentioning

confidence: 99%

Section: Geneticsmentioning

confidence: 99%

See 3 more Smart Citations

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

Lutova

Додуева

2019

BioComm

View full text Add to dashboard Cite

show abstract

Plant tumors: a hundred years of study

Додуева

Lebedeva

Kuznetsova

et al. 2020

Planta

Self Cite

View full text Add to dashboard Cite

show abstract

Gall mite Fragariocoptes setiger (Eriophyoidea) changes leaf developmental program and regulates gene expression in the leaf tissues of Fragaria viridis (Rosaceae)

Paponova

Chetverikov

Pautov

et al. 2017

Annals of Applied Biology

View full text Add to dashboard Cite

The interaction of plants with certain types of parasites leads to the formation of galls, organised structures that create the habitat of the parasite, caused by an abnormal proliferation of host plant's cells under the influence of growth regulators, secreted by the parasite, or by the plant itself under the influence of the parasite. Arthropods, mites in particular, are the largest group of gall-inducing phytoparasites, but the mechanisms of their interaction with plants remain virtually unexplored. The interaction of the gall-inducing eriophyoid mite Fragariocoptes setiger with Fragaria viridis plants was used as a model gall-mite system where data were obtained on the changes in the histological structure of F. viridis leaf blades under the influence of the mites as well as F. viridis gene expression during gall formation. For histological purposes, gall formation was split into four stages with each corresponding to the age of the gall as well as to specific changes that occur during that period. A dramatic change of adaxial-abaxial polarity of the lamina throughout the four stages was observed. Moreover, qRT-PCR analysis of F. viridis gene expression in the developing gall revealed changes in the expression levels of certain meristem-specific genes, as well as the genes that determine adaxial-abaxial polarity and signalling of phytohormones. Petersburg State University and the resource centre of the Komarov Botanical Institute of RAS.

show abstract

Initiation of spontaneous tumors in radish (Raphanus sativus): Cellular, molecular and physiological events

Cited by 20 publications

References 43 publications

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

Plant tumors: a hundred years of study

Gall mite Fragariocoptes setiger (Eriophyoidea) changes leaf developmental program and regulates gene expression in the leaf tissues of Fragaria viridis (Rosaceae)

Contact Info

Product

Resources

About