Initial cell maturity changes following transplantation in a hyaluronan-based hydrogel and impacts therapeutic success in the stroke-injured rodent brain

Payne, Samantha L.; Tuladhar, Anup; Obermeyer, Jaclyn M.; Varga, Balázs; Teal, Carter J.; Morshead, Cindi M.; Nagy, András; Shoichet, Molly S.

doi:10.1016/j.biomaterials.2018.11.020

Cited by 37 publications

(43 citation statements)

References 54 publications

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“…It is noteworthy that a decrease in the stroke infarct was also observed even in the absence of CsA, suggesting that CsA role is mostly related to cell proliferation . Except growth factors and immunosupressants, HA‐based hydrogels have been used for the delivery of various stem cells with or without the use of growth factors (e.g., VEGF), proteins (e.g., bone morphogenetic protein/BMP‐4), and various peptides (e.g., cathepsin K, glutamine, arginyl‐glycyl‐aspartic acid (RGD), and laminin‐derived) . Using all these approaches, neurogenesis and axogenesis were promoted through modulation of inflammation and through the migration of neural progenitor cells in the damaged tissue.…”

Section: Biomaterial‐based Approachesmentioning

confidence: 99%

Advanced Functional Materials and Cell‐Based Therapies for the Treatment of Ischemic Stroke and Postischemic Stroke Effects

Tapeinos

Larrañaga

Tomatis

et al. 2019

Adv Funct Materials

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Ischemic stroke is considered one of the most threatening neurological disorders with high percentages of mortality and morbidity worldwide. Although in recent years, several nanotechnological advances have improved the survival rates, severe untreated poststroke side‐effects continue to significantly influence the way of life of many. Tissue plasminogen activator and mechanical thrombectomy are considered the gold standards for the treatment of acute cerebral ischemia. These, however, fail to improve postischemic disorders. Herein, after a brief description of the pathophysiology of ischemic stroke and the following biochemical cascade, the most recent biomaterial and cell‐based strategies are reviewed for its treatment. Other therapeutics that have been proposed not only for the treatment of cerebral ischemia but also for the regeneration of the infarcted brain that is responsible for a variety of disorders, including cognitive, motor, and speech problems are also presented. Finally, a few reported studies on diagnostic and theranostic nanostructures are also provided.

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Section: Biomaterial‐based Approachesmentioning

confidence: 99%

Advanced Functional Materials and Cell‐Based Therapies for the Treatment of Ischemic Stroke and Postischemic Stroke Effects

Tapeinos

Larrañaga

Tomatis

et al. 2019

Adv Funct Materials

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“…In particular, an injectable, biocompatible, and biodegradable hydrogel scaffold composed of hyaluronan (HA) and methylcellulose (MC) (HAMC) is of particular interest for therapeutic cell delivery. The HAMC hydrogel is biodegradable, water soluble and provides a three-dimensional (3D) microenvironment for the cells, thereby enhancing cell viability ( Figure 1 A) [ 23 , 24 ]. Over the last decade, Shoichet lab has developed HAMC for various neurological disease models, such as ischemic stroke [ 24 ], retinal degeneration [ 25 ] and spinal cord injury [ 23 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…The HAMC hydrogel is biodegradable, water soluble and provides a three-dimensional (3D) microenvironment for the cells, thereby enhancing cell viability ( Figure 1 A) [ 23 , 24 ]. Over the last decade, Shoichet lab has developed HAMC for various neurological disease models, such as ischemic stroke [ 24 ], retinal degeneration [ 25 ] and spinal cord injury [ 23 , 26 ]. HAMC spatially localizes the drug or cells of interest at the site of delivery and promotes short-term controlled release of drugs to the central nervous system (CNS), with a degradation time of approximately 3 to 7 days in vivo [ 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair

Choi

Joshi

Payne

et al. 2020

IJMS

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Intervertebral disc (IVD) degeneration is one of the predominant causes of chronic low back pain (LBP), which is a leading cause of disability worldwide. Despite substantial progress in cell therapy for the treatment of IVD degeneration, significant challenges remain for clinical application. Here, we investigated the effectiveness of hyaluronan–methylcellulose (HAMC) hydrogels loaded with Wharton’s Jelly-derived mesenchymal stromal cell (WJ-MSCs) in vitro and in a rat coccygeal IVD degeneration model. Following induction of injury-induced IVD degeneration, female Sprague-Dawley rats were randomized into four groups to undergo a single intradiscal injection of the following: (1) phosphate buffered saline (PBS) vehicle, (2) HAMC, (3) WJ-MSCs (2 × 104 cells), and (4) WJ-MSCs-loaded HAMC (WJ-MSCs/HAMC) (n = 10/each group). Coccygeal discs were removed following sacrifice 6 weeks after implantation for radiologic and histologic analysis. We confirmed previous findings that encapsulation in HAMC increases the viability of WJ-MSCs for disc repair. The HAMC gel maintained significant cell viability in vitro. In addition, combined implantation of WJ-MSCs and HAMC significantly promoted degenerative disc repair compared to WJ-MSCs alone, presumably by improving nucleus pulposus cells viability and decreasing extracellular matrix degradation. Our results suggest that WJ-MSCs-loaded HAMC promotes IVD repair more effectively than cell injection alone and supports the potential clinical use of HAMC for cell delivery to arrest IVD degeneration or to promote IVD regeneration.

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“…Hyaluronan (HA), collagen, and synthetic peptides, such as RADA‐16 (PuraMatrix, PM), are all found to have these suitable properties when used as scaffolds for CNS cells in vitro and in vivo . Interactions between neuronal cells and ECM modify not only cell movement and adhesion but also neuronal cell differentiation and maturation .…”

Section: Introductionmentioning

confidence: 99%

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Ylä‐Outinen

Harju

Joki

et al. 2019

Macromolecular Bioscience

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There is a clear need for novel in vitro models, especially for neuronal applications. Development of in vitro models is a multiparameter task consisting of cell‐, biomaterial‐, and environment‐related parameters. Here, three different human origin neuronal cell sources are studied and cultured in various hydrogel 3D scaffolds. For the efficient evaluation of complex results, an indexing method for data is developed and used in principal component analysis (PCA). It is found that no single hydrogel is superior to other hydrogels, and collagen I (Col1) and hyaluronan–poly(vinyl alcohol) (HA1‐PVA) gels are combined into an interpenetrating network (IPN) hydrogel. The IPN gel combines cell supportiveness of the collagen gel and stability of the HA1‐PVA gel. Moreover, cell adhesion is studied in particular and it is found that adhesion of neurons differs from that observed for fibroblasts. In conclusion, the HA1‐PVA‐col1 hydrogel is a suitable scaffold for neuronal cells and supports adhesion formation in 3D.

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Initial cell maturity changes following transplantation in a hyaluronan-based hydrogel and impacts therapeutic success in the stroke-injured rodent brain

Cited by 37 publications

References 54 publications

Advanced Functional Materials and Cell‐Based Therapies for the Treatment of Ischemic Stroke and Postischemic Stroke Effects

Advanced Functional Materials and Cell‐Based Therapies for the Treatment of Ischemic Stroke and Postischemic Stroke Effects

An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

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