Inhibitory Effects of Methanol Extract from Nardostachys chinensis on 27-hydroxycholesterol-induced Differentiation of Monocytic Cells

Abstract

− 27-Hydroxycholesterol (27OHChol) has been reported to induce differentiation of monocytic cells into a mature dendritic cell phenotype. We examined the effect of methanol extract of Nardostachys chinensis (Nard) on 27OHChol-induced differentiation using THP-1, a human monocytic cell line. Treatment of monocytic cells with methanol extract of Nard resulted in decreased transcription and surface expression of CD80, CD83, and CD88 elevated by 27OHChol in a dose-dependent manner. Surface levels of MHC class I and II molecules elevated by 27OHChol were also reduced to basal levels by treatment with the Nard extract. Decreased endocytosis activity caused by 27OHChol was recovered by treatment with the Nard extract. CD197 expression and cell attachment were attenuated by the Nard extract. In addition, levels of transcription and surface expression of CD molecules involved in atherosclerosis, such as CD105, CD137, and CD166 upregulated by 27OHChol were significantly decreased by treatment with methanol extract of Nard. These results indicate that methanol extract of Nard down-regulates 27OHChol-induced differentiation of monocytic cells into a mature dendritic cell phenotype and expression of CD molecules associated with atherosclerosis. The current study suggests that biological activity of oxygenated cholesterol derivatives can be inhibited by herbal medication.

Preparation of Herbal Formulation for Inflammatory Bowel Disease Based on In Vitro Screening and In Vivo Evaluation in a Mouse Model of Experimental Colitis

“…U937 cells were prelabeled with BCECF/AM (10 μg/mL, Sigma, St. Louis, MO, USA) for 30 min at 37 °C. HT-29 cells (2 × 10 6 cells/well in 48-well plates)) were pretreated with sample (100 μg/mL) or 5-ASA (20 mM) for 1 h and then stimulated with TNF-α for 24 h [26]. Subsequently, HT-29 cells were co-incubated with BCECF/AM-prelabeled U937 cells (5 × 10 5 cells/well) for 30 min at 37 °C.…”

Anti-Angiogenic Effect of Asperchalasine A Via Attenuation of VEGF Signaling

“…The HUVECs (3 × 10 5 cells/well) were treated with asperchalasine A overnight. After 24 h, the total RNAs were prepared using an RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol [21]. Total RNA was then reverse-transcribed to cDNA using the AccuPower CycleScript RT premix (dT18) (Bioneer, Daejeon, Korea).…”