Inhibitory Effect of Hydroxysafflor Yellow A on Mouse Skin Photoaging Induced by Ultraviolet Irradiation

Abstract

Chronic exposure to ultraviolet (UV) irradiation is believed to be the major cause of skin damage that results in premature aging of the skin, so called photoaging, characterized by increases in skin thickness, formation of wrinkles, and loss of skin elasticity. UV induces damage to skin mainly by oxidative stress and collagen degradation. In this study, we examined the photo-protective effect of hydroxysafflor yellow A (HSYA), a major active chemical component isolated from Carthamus tinctorius L., by topical application on the skin of mice. Exposure of the dorsal depilated skin of mice to UV radiation four times a week for 10 weeks induced epidermal hyperplasia, elastin accumulation, collagen degradation, etc. HSYA at the doses of 50, 100, and 200 lg/mouse was topically applied immediately following each UV exposure. The effects of HSYA were evaluated by a series of tests, including macroscopic and histopathological evaluation of skin, pinch test, and redox homeostasis of skin homogenates. Results showed that the UV-induced skin damage was significantly improved after HSYA treatment, especially at doses of 100 and 200 lg/mouse. This protective effect is possibly related to the antioxidative property of HSYA and mediated by promoting endogenous collagen synthesis. This is the first study providing preclinical evidence for the protective effect of HSYA against photoaging.

Protective Effect of Super-Critical Carbon Dioxide Fluid Extract from Flowers and Buds ofChrysanthemum indicumLinnén Against Ultraviolet-Induced Photo-Aging in Mice

“…Hyp usually can be converted to the equivalent of collagen by multiplying the factor 7.46, because Hyp is the almost exclusive amino acid of collagen and accounts for 13.4 -0.24 percent of mammalian collagen in previous studies. 27,28 Determination of SOD, GSH-Px, CAT, and MDA in the skin The freshly removed skin tissue (0.4 gram) was homogenized (10,000 rpm, 20 sec) with an Ultra-Turrax homogenizer (T18 Basic, IKA) in 9 volumes of cold normal saline (4°C). Before centrifugation, 0.15 mL of the homogenate was taken out for the MDA assay and the rest was centrifuged at 3000 • g for 20 min at 4°C.…”

Andrographolide Sodium Bisulfate Prevents UV-Induced Skin Photoaging through Inhibiting Oxidative Stress and Inflammation

“…But if the balance of this oxidation/antioxidant system is broken by UV radiation, oxidative stress will occur in the deeper layers of the skin. Oxidative stress causes oxidative damage to cellular components in the skin, resulting in photodamage, photoaging, and photocarcinogenesis [ 12 , 13 ]. The nuclear factor kappa B (NF- κ B) signaling pathway mediated by ROS is an important cell signaling pathway in the process of skin inflammation and aging.…”

Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV‐Induced Photoaged Mice Skin

“…What is more, lipid peroxidation (LPO) is initiated by ROS on polyunsaturated fatty acids and aggravates LPO in membranes and cellular components and subsequently causes cell death and accumulation of abnormal proteins and cellular debris, finally leading to serious skin pathologies [33]. LPO can be further decomposed to a number of reactive aldehyde species such as MDA [34].…”