Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice

Abstract

The aim of this study was to investigate the antioxidant effects of flavonoid extract of lotus leaf (FELL) in vitro and in mice with gastric injury and elucidate the inhibitory effect of FELL on alcohol-induced gastric injury by antioxidant capacity. Gastric juice volume and acid value were measured in the established alcohol-induced acute gastric injury model in mice. Hematoxylin and eosin staining demonstrated the morphological changes in the gastric mucosa. FELL improved the alcohol-induced gastric lesions. Serological testing results showed that FELL significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO) and significantly reduced the levels of MDA and MPO in mice with gastric injury. Besides, FELL significantly reduced the levels of interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ). The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. Five compounds, including kaempferitrin, hyperoside, astragalin, phloridzin, and quercetin, were detected in FELL using high-performance liquid chromatography. Thus, FELL has an inhibitory effect on experimental alcohol-induced gastric injury because of the antioxidant capacity of the five compounds. Its effect increases as the FELL concentration increases, which is close to that of ranitidine. FELL, which is an active substance, provides a good gastroprotective effect.

Protective mechanism of fruit vinegar polyphenols against AGEs-induced Caco-2 cell damage

“…Yang et al found that apple polyphenols improved the antioxidant capacity of grass carp, protected grass carp from inflammation, and reduced the expression of IL-6 ( Yang et al, 2021 ). Chen et al found that lotus leaf flavonoids extract significantly reduced the levels of IL-6, IL-12 and TNF-α with the increase in concentration ( Chen et al, 2020 ). Our results indicated that orange vinegar and its main components could reduce the expression of inflammatory factors induced by CML.…”

Antioxidant and Inflammatory Effects of Nelumbo nucifera Gaertn. Leaves

“…Previous studies have shown that lotus leaf extract enhances the antioxidant capacity in alcohol-induced gastric injury mice [ 41 ] and high-fat diet-induced obese mice [ 42 ], including the ability to increase SOD, CAT, GSH, and GSH-PX and decrease the levels of MDA and NO. We further found that LLFE also significantly inhibits the levels of MDA and NO in D-Gal/LPS-induced mice and increases the levels of serum antioxidant enzymes and compounds.…”

Evaluation of Gastroprotective Effect of Betalain-Rich Ethanol Extract from Opuntia stricta var. Dillenii Employing an In Vivo Rat Model

“…Accordingly, recent research has focused on the search for efective plant-derived substances, which can be good sources for food additives as colorant, sweetener, preservative, and aroma [9][10][11][12][13][14][15][16]. Currently, there is a signifcant increase in the use of plant-derived colorants with a protective action against various perturbations and diseases such as diabetes, cancers, osteoporosis, and hyperlipidemia [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. It is notable that the ingestion of O. stricta prickly pears also improves platelet function and hemostatic balance, thus contributing to prevent atherosclerotic risks [33,34].…”