Inhibitory effect of endophyte bacteria on Botrytis cinerea and its influence to promote the grapevine growth

Barka, Essaïd Ait; Gognies, Sabine; Nowak, Jerzy; Audran, Jean‐Claude; Belarbi, A.

doi:10.1016/s1049-9644(02)00034-8

Cited by 229 publications

(131 citation statements)

References 16 publications

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“…Seed endophytes have been shown to colonize grass seedlings (Johnston-Monje et al 2014;Hardoim et al 2012;Rijavec et al 2007), travel within the plant and even exit the roots and colonize the rhizosphere (Johnston-Monje and Raizada 2011a; Hardoim et al 2012). Seed transmitted microbes colonizing the rhizosphere would be guaranteed first access to that habitat, perhaps creating a founder effect, blocking later colonization by less adapted soil microbes (Ait Barka et al 2002;Bacilio-Jimenez et al 2001;Raibaud et al 1980). Seed epiphytes should have no barrier to getting on root surfaces, however endophytes might struggle to exit the plant in order to gain access to the rhizosphere; a newly discovered mechanism endophytes might use to do this involves root border cells containing intracellular populations of bacteria that are sloughed off and released into the surrounding substrate almost like little water balloons full of inoculant (Cope-Selby 2013).…”

Section: Discussionmentioning

confidence: 99%

Bacterial populations in juvenile maize rhizospheres originate from both seed and soil

Johnston‐Monje

Lundberg

Lazarovits³

et al. 2016

Plant Soil

202

130

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Background and aims To assess the impacts of soil microbes and plant genotype on the composition of maize associated bacterial communities. Methods Two genotypes of Brazilian maize were planted indoors on sterile sand, a deep underground subsoil, and a nutrient-rich topsoil from the Amazon jungle (terra preta). DNA was extracted from rhizospheres, phyllospheres, and surface sterilized roots for 16S rDNA fingerprinting and next generation sequencing.Results Neither plant genotype nor soil type appeared to influence bacterial diversity in phyllospheres or endospheres. Rhizospheres showed strikingly similar 16S rDNA ordination of both fingerprinting and sequencing data, with soil type driving grouping patterns and genotype having a significant impact only on sterile sand. Rhizospheres grown in non-sterile soils contained greater bacterial diversity than sterile-sand grown ones, however the dominant OTUs (species of Proteobacteria and Bacteroidetes) were found in all rhizospheres suggesting seeds as a common source of inoculum. Rhizospheres of the commercial hybrid appeared to contain less bacterial diversity than the landrace. Conclusions Maize rhizospheres receive diverse bacteria from soil, are influenced by the genotype or treatment of the seed, and are dominated by species of Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. As many dominant 16S rDNA sequences were observed in rhizospheres grown in both sterile and non-sterile substrate, we conclude that the most common bacterial cells in juvenile maize rhizospheres are seed transmitted.

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Section: Discussionmentioning

confidence: 99%

Bacterial populations in juvenile maize rhizospheres originate from both seed and soil

Johnston‐Monje

Lundberg

Lazarovits³

et al. 2016

Plant Soil

202

130

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“…After 6 days, PsJN T also colonized stems and leaves endophytically. In addition to chickpea endophytic colonization, the potential of strain PsJN T was also shown with potato (Frommel et al, 1991), tomato (Pillay & Nowak, 1997) and grapevines (Ait Barka et al, 2002).…”

mentioning

confidence: 99%

“…Furthermore, plants inoculated with strain PsJN T were found to contain larger amounts of phenolics and chlorophyll , as well as increased levels of cytokinins (Lazarovits & Nowak, 1997) and enhanced activity of phenylalanine ammonia lyase . Strain PsJN T is also able to enhance resistance to low levels of potato pathogens (Nowak et al, 1995) and tomato pathogens (Sharma & Nowak, 1998) as well as to reduce in vitro infection of grapevine by Botrytis cinerea (Ait Barka et al, 2000Barka et al, , 2002. Recently, during a field experiment performed in The Netherlands, seven strains with high 16S rRNA gene sequence similarity to strain PsJN T were isolated (RG31-12, , mainly from the bulk and rhizosphere soil of maize and grass plants growing in an old grassland field (>50 years) (J. F. Salles and others, unpublished results).…”

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confidence: 99%

Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties

Sessitsch

Coenye

Sturz³

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

324

181

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A Gram-negative, non-sporulating, rod-shaped, motile bacterium, with a single polar flagellum, designated strain PsJN T , was isolated from surface-sterilized onion roots. This isolate proved to be a highly effective plant-beneficial bacterium, and was able to establish rhizosphere and endophytic populations associated with various plants. Seven related strains were recovered from Dutch soils. Based on 16S rRNA gene sequence data, strain PsJN T and the Dutch strains were identified as representing a member of the genus Burkholderia, as they were closely related to Burkholderia fungorum (98?7 %) and Burkholderia phenazinium (98?5 %). Analysis of whole-cell protein profiles and DNA-DNA hybridization experiments confirmed that all eight strains belonged to a single species. Strain PsJN T had a DNA G+C content of 61?0 mol%. Only low levels of DNA-DNA hybridization to closely related species were found. Qualitative and quantitative differences in fatty acid composition between strain PsJN T and closely related species were identified. The predominant fatty acids in strain PsJN T were 16 : 0, 18 : 1v7c and summed feature 3 (comprising 16 : 1v7c and/or iso-15 : 0 2-OH). Isolate PsJN T showed high 1-aminocyclopropane-1-carboxylate deaminase activity and is therefore able to lower the ethylene level in a developing or stressed plant. Production of the quorum-sensing signal Abbreviations: ACC, 1-aminocyclopropane-1-carboxylate; GFP, green fluorescent protein; NAHL, N-acyl-homoserine lactone.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of B. phytofirmans strains PsJN T , G44-5 and G6-5 are AY497470, AY836218 and AY836219.A neighbour-joining tree showing the position of B. phytofirmans sp. nov. within the genus Burkholderia, a dendrogram derived from the protein patterns of the strains studied and cross-sections showing chickpea roots with strain PsJN T

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“…Endophytes are ubiquitous, and every plant studied to date harbors at least one or more endophytic microbial species [30,33,35]. Antagonistic effects of bacterial endophytes on various pathogens of agriculturally important crops have been demonstrated [36][37][38]. However, the complexity of microbial interactions in natural environments is not well understood and search for biocontrol agents that are adequately effective in field applications remains important.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

A¹,

Mt²

2017

J Plant Pathol Microbiol

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Phytophthora blight, caused by Phytophthora capsici, is the most destructive disease of bell pepper in the United States. The effectiveness of current management strategies is limited by the long-term survival of oospores, wide host range, aggressive fungicide-resistant isolates, and lack of resistant cultivars with acceptable agronomic traits. Biological control is a viable alternative, and the use of endophytic microorganisms as biological control agents (BCA) has attracted widespread attention because they colonize the same ecological niches as plant pathogens. Seven isolates of bacterial endophytes in three genera Serratia (B17B), Enterobacter (E), and Bacillus (IMC8, Y, Ps, Psl and Prt), isolated from papaya, snap bean and flowering dogwood were evaluated for effects on P. capsici mycelial growth in vitro, and on disease severity and plant growth in greenhouse environment. All isolates significantly inhibited the mycelial growth of P. capsici with Ps, Psl and Prt having superior inhibitory effects. Seed treatments with Ps, Psl and Prt followed by plant inoculation with P. capsici reduced disease severity, and significantly increased plant shoot height, fresh weight, and fruit yield (number and weight) with Ps and Prt being slightly superior to Psl. Compatibility tests between isolates showed that only B17B and Y were compatible with each other. Sensitivity tests to different levels of mefenoxam (Ridomil Gold SL) showed that Ps, Psl and Prt were tolerant to the fungicide, while P. capsici was highly sensitive. Thus, Ps, Psl and Prt can be used in rotation with mefenoxam to reduce the frequency of fungicide usage for a more environmentally friendly, long lasting, consistent, and effective control of phytophthora blight. Overall, all isolates tested are potential BCAs against P. capsici, but more greenhouse and field studies are required to confirm in vitro results for all isolates that showed good potential in in vitro studies.

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Inhibitory effect of endophyte bacteria on Botrytis cinerea and its influence to promote the grapevine growth

Cited by 229 publications

References 16 publications

Bacterial populations in juvenile maize rhizospheres originate from both seed and soil

Bacterial populations in juvenile maize rhizospheres originate from both seed and soil

Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

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