Inhibitory Activities ofStauntonia hexaphyllaLeaf Constituents on Rat Lens Aldose Reductase and Formation of Advanced Glycation End Products and Antioxidant

Abstract

Stauntonia hexaphylla (Thunb.) Decne. (Lardizabalaceae) leaves (SHL) have been used traditionally as analgesics, sedatives, diuretics, and so on, in China. To date, no data have been reported on the inhibitory effect of SHL and its constituents on rat lens aldose reductase (RLAR) and advanced glycation end products (AGEs). Therefore, the inhibitory effect of compounds isolated from SHL extract on RLAR and AGEs was investigated to evaluate potential treatments of diabetic complications. The ethyl acetate (EtOAC) fraction of SHL extract showed strong inhibitory activity on RLAR and AGEs; therefore, EtOAc fraction (3.0 g) was subjected to Sephadex LH-20 column chromatography, for further fractionation, with 100% MeOH solvent system to investigate its effect on RLAR and AGEs. Phytochemical investigation of SHL led to the isolation of seven compounds. Among the isolated compounds, chlorogenic acid, calceolarioside B, luteolin-3′-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, and luteolin-7-O-β-D-glucopyranoside exhibited significant inhibitory activity against RLAR with IC50 in the range of 7.34–23.99 μM. In addition, 3-(3,4-dihydroxyphenyl) propionic acid, neochlorogenic acid, and luteolin-3′-O-β-D-glucopyranoside exhibited the most potent inhibitory activity against formation of AGEs, with an IC50 value of 115.07–184.06 μM, compared to the positive control aminoguanidine (820.44 μM). Based on these findings, SHL dietary supplements could be considered for the prevention and/or treatment of diabetes complication.

Anti-glycation, Carbonyl Trapping and Anti-inflammatory Activities of Chrysin Derivatives

“…Bovine serum albumin (50 mg/mL) was incubated at 37 °C for 48 h with methylglyoxal (100 mM) in sodium phosphate buffer (0.1 M, pH 7.4) containing 0.2 g/L NaN 3 in the presence of various concentrations of the compounds (including a control) [ 38 ].…”

Changes in the Content of Phenolic Compounds and Biological Activity in Traditional Mexican Herbal Infusions with Different Drying Methods

“…Furthermore, the antioxidant activity of herbs and spices, associated with type 2 diabetes, may be a primary factor in vascular diseases that diabetics often develop [8]. Due to the different effects of advanced protein glycation in diabetes and the pathologies caused by age, it has been proposed that the inhibition of glycation can prevent the progression of different pathologies of diabetic complications and delay aging [9][10][11]. Therefore, studies have focused on the characterization of phenolic and other phytochemical compounds with biological activity, which are supplied to human organisms as food components or as specific preventive pharmaceutical products, and can improve glucose metabolism, as well as the general health of diabetic patients [12,13].…”

Anti-Inflammatory Effects of a Stauntonia hexaphylla Fruit Extract in Lipopolysaccharide-Activated RAW-264.7 Macrophages and Rats by Carrageenan-Induced Hind Paw Swelling

“…The plant is widely distributed in Korea, Japan and China [ 11 , 12 ]. The known chemical compounds of S. hexaphylla include triterpenoids, glucosides, flavonoids, and phenylpropanoids [ 13 ]. However, the anti-inflammatory effects and the mechanism of the S. hexaphylla fruit (SHF) extracts have not been reported.…”