Inhibitors of mitochondrial ATP production at the time of compaction improve development of in vitro produced porcine embryos

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

“…In the current study, which is similar to that of Harvey et al (2004), no beneficial effect was evident, even in the presence of 1.5 mM glucose or under conditions that likely elicit stress (0 or 4 mM glucose). This outcome contrasts with previous reports of improved development of blastocysts cultured from the morula stage in the presence of 1.5 mM glucose and 10 mM DNP Machaty et al, 2001;Rieger et al, 2002;Barcelo-Fimbres and Seidel, 2007). This disparity in outcome following DNP treatment may be related to the composition of embryo culture media (De La Torre-Sanchez et al, 2006b) or with differing embryo density.…”

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

“…In the current study, which is similar to that of Harvey et al (2004), no beneficial effect was evident, even in the presence of 1.5 mM glucose or under conditions that likely elicit stress (0 or 4 mM glucose). This outcome contrasts with previous reports of improved development of blastocysts cultured from the morula stage in the presence of 1.5 mM glucose and 10 mM DNP Machaty et al, 2001;Rieger et al, 2002;Barcelo-Fimbres and Seidel, 2007). This disparity in outcome following DNP treatment may be related to the composition of embryo culture media (De La Torre-Sanchez et al, 2006b) or with differing embryo density.…”

A role for the Warburg effect in preimplantation embryo development: Metabolic modification to support rapid cell proliferation

“…Several independent publications support the existence of the WE in embryos. Inhibition of TCA cycle activity at the time of embryo compaction improves pig and cow embryo development (Thompson et al, 2000;Machaty et al, 2001;Rieger et al, 2002), potentially by reducing ATP production from glucose and forcing glucose metabolism, via the PPP, to establish a favorable redox state within the embryo (Thompson et al, 2000). Stimulating PPP activity in 8-16 cell bovine embryos increases glucose metabolism without affecting lactate production, and reduces lipid accumulation (De La Torre-Sanchez et al, 2006), which is consistent with the WE.…”

Transcriptional Profiling of Pig Embryogenesis by Using a 15-K Member Unigene Set Specific for Pig Reproductive Tissues and Embryos1

“…Mutations in humans cause maple syrup Urine disease type 3 [40]. Dihydrolipoamide dehydrogenase precursor is also an ubiquinone reductase and thus is involved with protecting against lipid and protein peroxidation [41,42], an important observation because embryos created in vitro appear to be oxidatively stressed [43]. This clone (pd6end2-005-D03) was not detected by Whitworth et al [10] but found here to increase from the oocyte to four-cell to blastocyst stage.…”

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

“…In the current study, which is similar to that of Harvey et al (2004), no beneficial effect was evident, even in the presence of 1.5 mM glucose or under conditions that likely elicit stress (0 or 4 mM glucose). This outcome contrasts with previous reports of improved development of blastocysts cultured from the morula stage in the presence of 1.5 mM glucose and 10 mM DNP Machaty et al, 2001;Rieger et al, 2002;Barcelo-Fimbres and Seidel, 2007). This disparity in outcome following DNP treatment may be related to the composition of embryo culture media (De La Torre-Sanchez et al, 2006b) or with differing embryo density.…”

A role for the Warburg effect in preimplantation embryo development: Metabolic modification to support rapid cell proliferation

“…Several independent publications support the existence of the WE in embryos. Inhibition of TCA cycle activity at the time of embryo compaction improves pig and cow embryo development (Thompson et al, 2000;Machaty et al, 2001;Rieger et al, 2002), potentially by reducing ATP production from glucose and forcing glucose metabolism, via the PPP, to establish a favorable redox state within the embryo (Thompson et al, 2000). Stimulating PPP activity in 8-16 cell bovine embryos increases glucose metabolism without affecting lactate production, and reduces lipid accumulation (De La Torre-Sanchez et al, 2006), which is consistent with the WE.…”

Transcriptional Profiling of Pig Embryogenesis by Using a 15-K Member Unigene Set Specific for Pig Reproductive Tissues and Embryos1

“…Mutations in humans cause maple syrup Urine disease type 3 [40]. Dihydrolipoamide dehydrogenase precursor is also an ubiquinone reductase and thus is involved with protecting against lipid and protein peroxidation [41,42], an important observation because embryos created in vitro appear to be oxidatively stressed [43]. This clone (pd6end2-005-D03) was not detected by Whitworth et al [10] but found here to increase from the oocyte to four-cell to blastocyst stage.…”

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

“…In the current study, which is similar to that of Harvey et al (2004), no beneficial effect was evident, even in the presence of 1.5 mM glucose or under conditions that likely elicit stress (0 or 4 mM glucose). This outcome contrasts with previous reports of improved development of blastocysts cultured from the morula stage in the presence of 1.5 mM glucose and 10 mM DNP Machaty et al, 2001;Rieger et al, 2002;Barcelo-Fimbres and Seidel, 2007). This disparity in outcome following DNP treatment may be related to the composition of embryo culture media (De La Torre-Sanchez et al, 2006b) or with differing embryo density.…”

A role for the Warburg effect in preimplantation embryo development: Metabolic modification to support rapid cell proliferation

“…Several independent publications support the existence of the WE in embryos. Inhibition of TCA cycle activity at the time of embryo compaction improves pig and cow embryo development (Thompson et al, 2000;Machaty et al, 2001;Rieger et al, 2002), potentially by reducing ATP production from glucose and forcing glucose metabolism, via the PPP, to establish a favorable redox state within the embryo (Thompson et al, 2000). Stimulating PPP activity in 8-16 cell bovine embryos increases glucose metabolism without affecting lactate production, and reduces lipid accumulation (De La Torre-Sanchez et al, 2006), which is consistent with the WE.…”

Transcriptional Profiling of Pig Embryogenesis by Using a 15-K Member Unigene Set Specific for Pig Reproductive Tissues and Embryos1

“…Mutations in humans cause maple syrup Urine disease type 3 [40]. Dihydrolipoamide dehydrogenase precursor is also an ubiquinone reductase and thus is involved with protecting against lipid and protein peroxidation [41,42], an important observation because embryos created in vitro appear to be oxidatively stressed [43]. This clone (pd6end2-005-D03) was not detected by Whitworth et al [10] but found here to increase from the oocyte to four-cell to blastocyst stage.…”