Inhibition of Vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (BLIS) produced by Vibrio mediterranei 1

Carraturo, Antonio; Raieta, K.; Ottaviani, Donatella; Russo, Gian Luigi

doi:10.1111/j.1365-2672.2006.02909.x

Cited by 42 publications

(27 citation statements)

References 39 publications

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“…Some BLIS are also resistant to treatment with organic solvents. For example, BLIS-400 is resistant to organic chemicals including ethanol, methanol, acetone, and chloroform (Carraturo et al, 2006). This feature is similar to some bacteriocins isolated from Gram-positive bacteria and Archaea (Carraturo et al, 2006).…”

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 72%

“…For example, BLIS-400 is resistant to organic chemicals including ethanol, methanol, acetone, and chloroform (Carraturo et al, 2006). This feature is similar to some bacteriocins isolated from Gram-positive bacteria and Archaea (Carraturo et al, 2006). Finally, most BLIS from marine bacteria are produced during the stationary phase of growth, similar to bacteriocins from Gram-positive bacteria (Pinto et al, 2009;Tahiri et al, 2004).…”

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 72%

“…These bacteriocins share similar characteristics with the Class II bacteriocins of Gram-positive bacteria. (Table 3) (Bagenda et al, 2008;Carraturo et al, 2006;Longeon et al, 2004;Messi et al, 2003;Moro et al, 1997;Pinto et al, 2009;Pirzada et al, 2004;Prasad et al, 2005;Rajaram et al, 2010;Satish Kumar & Arul, 2009;Selvin et al, 2004;Shehane & Sizemore, 2002;Sugita et al, 1997;Valenzuela et al, 2010;Zai et al, 2009). These species are important in the seafood industry and in human health.…”

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 99%

“…The identification of harveyicin led to numerous bacteriocin-screening studies in marine bacteria, which focused primarily on biochemical characterization of bacteriocins and bacteriocin-like inhibitory substances (Bagenda et al, 2008;Bhugaloo-Vial et al, 1996;Carraturo et al, 2006;Hosseini et al, 2009;Hoyt & Sizemore, 1982;Longeon et al, 2004;McCall & Sizemore, 1979;Messi et al, 2003;Metivier et al, 1998;Moro et al, 1997;Nilsson et al, 2002;Pinto et al, 2009;Pirzada et al, 2004;Prasad et al, 2005;Rajaram et al, 2010;Selvin et al, 2004;Shehane & Sizemore, 2002;Stoffels et al, 1992;Sugita et al, 1997;Suzuki et al, 2005;Tahiri et al, 2004;Valenzuela et al, 2010;Zai et al, 2009). The majority of these studies focused on the killing breadth of the producing strains due to their potential for use as antimicrobials and probiotics.…”

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 99%

See 3 more Smart Citations

Bacteriocins of Aquatic Microorganisms and Their Potential Applications in the Seafood Industry

Bakkal¹,

Robinson²,

Riley³

2012

Health and Environment in Aquaculture

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Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 72%

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 72%

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 99%

Section: Bacteriocins Of Marine Bacteriamentioning

confidence: 99%

See 2 more Smart Citations

Bacteriocins of Aquatic Microorganisms and Their Potential Applications in the Seafood Industry

Bakkal¹,

Robinson²,

Riley³

2012

Health and Environment in Aquaculture

View full text Add to dashboard Cite

“…[13][14][15] In this study, the isolate HG-3F, closely related to V. parahaemolyticus (97.6% similarity), showed inhibitory activity only against indicator strains of V. parahaemolyticus and V. splendidus. Moreover, the isolate HG-12F, identified as A. fischeri (100% similarity), showed inhibitory activity only against indicator strains of V. ichthyoenteri, V. parahaemolyticus and V. splendidus.…”

Section: Cfb Group Of Bacteroidetes Actinobacteriamentioning

confidence: 99%

Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus)

et al. 2010

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Seahorse populations have declined in the last years, largely due to overfishing and habitat destruction. 1 Recent research efforts have, therefore, been focused to provide a better biological knowledge of these species. We have recently studied, as part of the Hippocampus project, wild populations of seahorse (Hippocampus guttulatus) in some areas of the Spanish coast and established breeding programs in captivity. 2 With the development of intensive production methods, it has become apparent that diseases can be a significant limiting factor. Vibrio species are among the most important bacterial pathogens of marine fish. They are responsible for several diseases, and high mortalities due to vibriosis have been reported. 3,4 Thus, we have screened marine bacteria isolated from the intestinal content and cutaneous mucus of seahorses (H. guttulatus) for production of antibacterial compounds against pathogenic Vibrio species. This mechanism of competition offers the possibility of using these antagonistic microorganisms as biological control agents. [5][6][7] Adult seahorses (n¼8) were collected from the coast of Galicia (NW Spain). The culturable microbiota was isolated from the intestinal content and cutaneous mucus as follows. Intestinal content from each seahorse was collected, weighed homogenized using tissue grinders, and vortexed vigorously in sterile saline solution (8.5 g l À1 NaCl), whereas the cutaneous mucus was collected from the dorsal surface with a sterile cotton swab into a small amount of sterile saline solution. Tenfold serial dilutions of samples were prepared and plated on marine agar (Difco, Detroit, MI, USA), tryptic soy agar supplemented with 15 g l À1 NaCl (Cultimed, Barcelona, Spain) and Cytophaga agar prepared with 50% seawater (0.5 g l À1 tryptone, 0.5 g l À1 yeast extract, 0.2 g l À1 sodium acetate, 15 g l À1 agar and adjusted to pH 7.2). All plates were incubated for 3-7 days at 20 1C. Colonies with different morphological characteristics from each sample were selected, subcultured in suitable media and stored in sterile glycerol (15% v/v) at À80 1C.To test the ability of the isolates to inhibit growth of pathogenic Vibrio strains (Table 1), we grew all isolates on suitable agar media at 20 1C for 2-3 days. After incubation, we spotted a loop of each isolate onto the surface of marine agar previously inoculated with overnight cultures of the indicator strain. Clear zones after overnight incubation at 20 1C indicated the presence of antibacterial substances.Bacterial isolates showing antagonistic activity against pathogenic Vibrio strains were identified using the 16S rRNA gene, amplified from extracted genomic DNA with primers 27F and 907R and Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA). 8 PCR (95 1C for 10 min; 30 cycles of 94 1C for 30 s, 50 1C for 1 min and 72 1C for 2 min; and 72 1C for 10 min) yielded products of approximately 0.9 kb, which underwent sequencing. The sequences obtained were compared to those available in the GenBank, EMBL and DDBJ databases with the BLAST p...

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Environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic Vibrio cholerae and Vibrio parahaemolyticus strains

et al. 2017

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With the overuse of antibiotics, many pathogens including Vibrio cholerae and Vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. While understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of V. cholerae and V. parahaemolyticus could provide insight to their susceptibility outside of the human host. Based on previous work showing competition among environmental strains, we predict that marine‐derived bacteria should inhibit Vibrio pathogens and may be a source of unique antibiotic compounds. We tested a collection of 3,456 environmental Vibrio isolates from diverse habitats against a panel of V. cholerae and V. parahaemolyticus, and identified 102 strains that inhibited the growth of these pathogens. Phylogenetic analysis revealed that 40 pathogen‐inhibiting strains were unique at the hsp60 gene sequence while 62 of the isolates were identical suggesting clonal groups. Genomic comparisons of ten strains revealed diversity even between clonal isolates and were identified as being closely related to known Vibrio crassostreae, Vibrio splendidus, and Vibrio tasmaniensis strains. Further analysis revealed multiple biosynthetic gene clusters within all sequenced genomes that encoded secondary metabolites with potential antagonistic activity. Thus, environmental vibrios represent a source of compounds that inhibit Vibrio pathogens.

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Inhibition of Vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (BLIS) produced by Vibrio mediterranei 1

Cited by 42 publications

References 39 publications

Bacteriocins of Aquatic Microorganisms and Their Potential Applications in the Seafood Industry

Bacteriocins of Aquatic Microorganisms and Their Potential Applications in the Seafood Industry

Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus)

Environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic Vibrio cholerae and Vibrio parahaemolyticus strains

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