Inhibition of the Splicing of Glucose-6-phosphate Dehydrogenase Precursor mRNA by Polyunsaturated Fatty Acids

Tao, Huimin; Szeszel-Fedorowicz, Wioletta; Amir-Ahmady, Batoul; Gibson, Matthew; Stabile, Laura P.; Salati, Lisa M.

doi:10.1074/jbc.m203196200

Cited by 41 publications

(64 citation statements)

References 45 publications

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“…This large change in mRNA accumulation makes this an excellent dietary paradigm in which to study the molecular details involved in regulated splicing. Our work has identified a cis-acting regulatory element in exon 12 of the G6PD mRNA that is required for regulated splicing of this RNA [19]. In this report, we present evidence that hnRNP K, L, and A2/B1 differentially bind to exon 12 in response to starvation and refeeding.…”

Section: Discussionsupporting

confidence: 54%

“…Inclusion of exon 12 and its surrounding introns was required for the inhibition of reporter RNA expression by polyunsaturated fatty acids. Furthermore, this exon when ligated to a heterologous RNA confers regulation by nutrients to this RNA [19]. These characteristics are consistent with exon 12 containing an enhancer or a silencer element.…”

Section: Introductionsupporting

confidence: 59%

“…(A) The G6PD gene contains 13 exons and 12 introns of which exon 12 is known to regulate splicing in primary hepatocytes [19]. The exon 12 probe contained the entire exon 12 sequence.…”

Section: Discussionmentioning

confidence: 99%

“…2B). The first 37 nt of exon 12 are not required for regulated splicing of G6PD mRNA in rat hepatocytes [19].…”

Section: The 60 Kda and 37 Kda Proteins Bind To Nt 65-79 Of Exon 12mentioning

confidence: 99%

“…Exon 12 contains a regulatory element involved in regulated splicing of G6PD pre-mRNA [19]. Therefore, we used exon 12 as a probe (Fig.…”

Section: Starvation Enhances Binding Of Proteins To Exon 12 Rnamentioning

confidence: 99%

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Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing

Griffith¹,

Walsh²,

Szeszel-Fedorowicz³

et al. 2006

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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SummaryNutrient regulation of glucose-6-phosphate dehydrogenase (G6PD) expression occurs through changes in the rate of splicing of G6PD pre-mRNA. This posttranscriptional mechanism accounts for the 12-to 15-fold increase in G6PD expression in livers of mice that were starved and then refed a high-carbohydrate diet. Regulation of G6PD pre-mRNA splicing requires a cis-acting element in exon 12 of the pre-mRNA. Using RNA probes to exon 12 and nuclear extracts from livers of mice that were starved or refed, proteins of 60 kDa and 37 kDa were detected bound to nucleotides 65-79 of exon 12 and this binding was decreased by 50% with nuclear extracts from refed mice. The proteins were identified as hnRNP K, and L, and hnRNP A2/B1 by LC-MS/MS. The decrease in binding of these proteins to exon 12 during refeeding was not accompanied by a decrease in the total amount of these proteins in total nuclear extract. HnRNPs K, L and A2/B1 have known roles in the regulation of mRNA splicing. The decrease in binding of these proteins during treatments that increase G6PD expression is consistent with a role for these proteins in the inhibition of G6PD mRNA splicing.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionsupporting

confidence: 59%

“…(A) The G6PD gene contains 13 exons and 12 introns of which exon 12 is known to regulate splicing in primary hepatocytes [19]. The exon 12 probe contained the entire exon 12 sequence.…”

Section: Discussionmentioning

confidence: 99%

“…2B). The first 37 nt of exon 12 are not required for regulated splicing of G6PD mRNA in rat hepatocytes [19].…”

Section: The 60 Kda and 37 Kda Proteins Bind To Nt 65-79 Of Exon 12mentioning

confidence: 99%

“…Exon 12 contains a regulatory element involved in regulated splicing of G6PD pre-mRNA [19]. Therefore, we used exon 12 as a probe (Fig.…”

Section: Starvation Enhances Binding Of Proteins To Exon 12 Rnamentioning

confidence: 99%

See 3 more Smart Citations

Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing

Griffith¹,

Walsh²,

Szeszel-Fedorowicz³

et al. 2006

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

Self Cite

View full text Add to dashboard Cite

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Free-choice high-fat diet alters circadian oscillation of energy intake in adolescent mice: role of prefrontal cortex

et al. 2016

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Our data show that the PFC participates in driving motivational feeding during HFD-evoked hyperphagia and also suggest that sensory neural pathways might be relevant for the onset of eating disorders and overweight. Moreover, we have observed that animals that had the possibility of choosing between standard chow and HFD were more hyperphagic and specifically displayed an overexpression of the tyrosine hydroxylase gene.

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Role of fish oil in human health and possible mechanism to reduce the inflammation

Ellulu

Khaza’ai

Abed³

et al. 2015

Inflammopharmacol

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The roles of Omega-3 FAs are inflammation antagonists, while Omega-6 FAs are precursors for inflammation. The plant form of Omega-3 FAs is the short-chain α-linolenic acid, and the marine forms are the long-chain fatty acids: docosahexaenoic acid and eicosapentaenoic acid. Omega-3 FAs have unlimited usages, and they are considered as omnipotent since they may benefit heart health, improve brain function, reduce cancer risks and improve people's moods. Omega-3 FAs also have several important biological effects on a range of cellular functions that may decrease the onset of heart diseases and reduce mortality among patients with coronary heart disease, possibly by stabilizing the heart's rhythm and by reducing blood clotting. Some review studies have described the beneficial roles of Omega-3 FAs in cardiovascular diseases (CVDs), cancer, diabetes, and other conditions, including inflammation. Studies of the effect of Omega-3 FAs gathered from studies in diseased and healthy population. CVDs including atherosclerosis, coronary heart diseases, hypertension, and metabolic syndrome were the major fields of investigation. In studies of obesity, as the central obesity increased, the level of adipocyte synthesis of pro-inflammatory cytokines like tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) were increased and the level of anti-inflammatory adiponectin was decreased indicating a state of inflammation. The level of C reactive protein (CRP) synthesized from hepatocyte is increased by the influence of IL-6. CRP can be considered as a marker of systemic inflammation associated with increased risks of CVDs. In molecular studies, Omega-3 FAs have direct effects on reducing the inflammatory state by reducing IL-6, TNF-α, CRP and many other factors. While the appropriate dosage along with the administrative duration is not known, the scientific evidence-based recommendations for daily intake are not modified.

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Inhibition of the Splicing of Glucose-6-phosphate Dehydrogenase Precursor mRNA by Polyunsaturated Fatty Acids

Cited by 41 publications

References 45 publications

Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing

Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing

Free-choice high-fat diet alters circadian oscillation of energy intake in adolescent mice: role of prefrontal cortex

Role of fish oil in human health and possible mechanism to reduce the inflammation

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