Inhibition of the growth of Paenibacillus larvae, the causal agent of American foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources

Alippi, Adriana Mónica; Reynaldi, Francisco José

doi:10.1016/j.jip.2005.12.002

Cited by 82 publications

(78 citation statements)

References 28 publications

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“…B. amyloliquefaciens resulted the species with the highest number of isolates. Several studies reported the isolation of Bacillus species in honeys (Iurlina and Fritz, 2005;Alippi and Reynaldi, 2006) but this study represents the first report on the identification of Bacillus spp. in honeydew honeys.…”

Section: Discussionmentioning

confidence: 89%

“…However, thanks to the ability of several Bacillus strains to produce antibiotics, bacteriocins, or antifungal compounds, they find application to pursue agricultural and healthcare purposes (Alfonzo et al, 2012;Duc et al, 2004). Some strains of P. polymyxa (Lee et al, 2009), B. subtilis (Sabatè et al, 2009), B. cereus, B. licheniformis and B. megaterium (Alippi and Reynaldi, 2006), isolated from honey samples or different apiarian sources, were successfully tested against Paenibacillus larvae. B. amyloliquefaciens is considered able to inhibit Ascosphaera apis (Brittes Benitez et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Cultivable microorganisms associated with honeys of different geographical and botanical origin

et al. 2014

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a b s t r a c tIn this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-ITS rRNA region for filamentous fungi. The results showed the presence of 13 species of bacteria, 5 of yeasts and 17 of filamentous fungi; the species most frequently isolated were Bacillus amyloliquefaciens, Zygosaccharomyces mellis and Aspergillus niger for the three microbial groups, respectively. The highest microbial diversity was found in multifloral honeys. No correlation among the microbial species and the botanical/geographical origin was found, but some strains were highly adapted to these matrices since they were found in several samples of different origin.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Cultivable microorganisms associated with honeys of different geographical and botanical origin

et al. 2014

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“…Assay for pathogen inhibitory activity. Pathogen inhibitory activity of the promising enzyme-producing isolates were primarily tested by 'co-culture' (cross streaking) method (Alippi and Reynaldi 2006) and the strains that showed antagonism against ≥1 studied Aeromonas spp. were further evaluated by the 'double-layer' method (Dopazo et al 1988) with minor modifi cation.…”

Section: Methodsmentioning

confidence: 99%

Selection and probiotic characterization of exoenzyme-producing bacteria isolated from the gut of Catla catla (Actinopterygii: Cypriniformes: Cyprinidae)

Dutta¹

2015

Acta Ichthyol. Piscat.

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. 2015. Selection and probiotic characterization of exoenzyme-producing bacteria isolated from the gut of Catla catla (Actinopterygii: Cypriniformes: Cyprinidae). Acta Ichthyol. Piscat. 45 (4): 373-384.Background. Exoenzyme-producing gut microbiota and their likely use as probionts have been widely investigated in major carps. However, reports on exoenzyme-producing capacity together with inhibition of fi sh pathogens are scarce. The presently reported study aimed at characterization of novel probionts from the gastro-intestinal (GI) tract of an Indian major carp-catla, Catla catla (Hamilton, 1822) depending upon extracellular enzyme-production, antagonism against Aeromonas spp., growth in fi sh mucus, bile tolerance, and bio-safety to the target fi sh. Materials and methods. GI tracts were divided into proximal and distal parts, homogenized, and plated onto selective media plates following enrichment culture technique. Exoenzyme-producing isolates were selected through qualitative and quantitative assay of 6 enzymes. Inhibition of Aeromonas spp. was tested through 'cross-streaking' and 'double-layer' method. Tolerance to GI condition was determined by growth in sterilized fi sh mucus and exposure to bile juice. Bio-safety evaluation was done by intra-peritoneal injection of live candidate probiotics and co-culture with autochthonous gut bacteria. Further, 16S rDNA fragments from the putative probiotics were sequenced, edited, analysed, identifi ed, and deposited to the GenBank. Results. Seventeen potent isolates were primarily selected through the ability of producing extracellular enzymes, viz., amylase, protease, lipase, cellulase, phytase, and xylanase. Further study portraying antagonism against Aeromonas salmonicida, A. hydrophila, and A. veronii led to select the strains CCH1A and CCH1Ph as putative probiotics. Both the strains were competent to grow in intestinal mucus and could resist diluted bile juice (9% and 10.5%, respectively). The isolates did not produce any disease symptom or mortality in Catla catla fi ngerlings during the 21 day trial and were competent to grow with other autochthonous isolates. On the basis of phenotypic characteristics and 16S rDNA sequence analysis, the strains CCH1A and CCHIPh were identifi ed as Bacillus aerius (KF623288) and Bacillus sonorensis (KF623289), respectively. Conclusion. Exoenzyme-producing gut bacteria may inhibit the growth of pathogenic Aeromonas spp. and tolerate gastrointestinal condition. Further research is inevitable to explore their potentialities in aquaculture.

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“…Inhibitory activities of each isolate were assayed against the M. plutonius DAT561 strain, which exhibited virulence in larvae reared in vitro (Arai et al 2012), by using a modification of a previously described diffusion technique (Alippi and Reynaldi 2006). The M. plutonius DAT561 strain was cultured in KSBHI medium (BHI medium plus 0.15 M KH 2 PO 4 and 1% soluble starch) for 5 d at 35°C under anaerobic conditions in an AnearoPack System (Mitsubishi Gas Chemical Co. Inc., Tokyo, Japan).…”

Section: In Vitro Inhibition Assays For M Plutoniusmentioning

confidence: 99%

Bacterial Probiotics Induce an Immune Response in the Honey Bee (Hymenoptera: Apidae)

Evans

Lopez

2004

Journal of Economic Entomology

195

107

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Inhibition of the growth of Paenibacillus larvae, the causal agent of American foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources

Cited by 82 publications

References 28 publications

Cultivable microorganisms associated with honeys of different geographical and botanical origin

Cultivable microorganisms associated with honeys of different geographical and botanical origin

Selection and probiotic characterization of exoenzyme-producing bacteria isolated from the gut of Catla catla (Actinopterygii: Cypriniformes: Cyprinidae)

Bacterial Probiotics Induce an Immune Response in the Honey Bee (Hymenoptera: Apidae)

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