Inhibition of Protein Denaturation by Extracts of Leaves, Stems and Roots of Pisonia Grandis R.BR.

Abstract

Pisonia grandis R.Br. is a rural folkloric medicinal plant of Nyctaginaceae family and has been extensively used as an anti-diabetic and antiinflammatory agent.The present study is aimed to evaluate the in-vitro anti-arthritic activity of the solvent extracts and the column fractionates of this plant. Denaturation of tissue protein is a proven cause of inflammatory and arthritic diseases. Inhibition of protein denaturation is a measure of anti arthritic activity. The petroleum-ether and ethanol extract concentrates of leaves, stems and roots of Pisonia grandis exhibited inhibition of protein denaturation. Among the extract concentrates of Pisonia grandis the non-polar extract concentrate of leaves of Pisonia grandis exhibited a 90 % inhibition of protein denaturation at 50 µg/ml concentration, comparable with that of standard drug Diclofenac sodium. The commercially well-known anti-arthritic drug palmitic acid showed 76% inhibition only at 800µg/ml. Column chromatographic analysis of petroleum ether extract of Pisonia grandis led to the isolation of stigmasterol which showed 66% inhibition of protein denaturation at 800µg/ml.

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“…31 . In-vitro assessment of anti-arthritic potential of pet-ether and ethanol extract of leaves, stems and root was carried out by Poongothai et al 32 The study revealed that the petether and ethanol extracts of leaves exhibited significant anti-arthiritic potential equivalent to that of palmitic acid at the same concentration. In yet another assay of a biherbal extract composed of ethanolic extract of Pisonia grandis and cardiospermum helicacabum, indicated a greater inhibition of protein denaturation compared to the individual extracts 33 .…”