Inhibition of phospholipase C activity by auriculatin and 8‐prenylluteone isolated from Erythrina senegalensis

Abstract

Auriculatin and 8‐prenylluteone were isolated from Erythrina senegalensis (Leguminosae) as inhibitors of phospholipase C (PLC) and phosphoinositides (PI)‐turnover in PLC‐γ1 overexpressing NIH3T3 fibroblasts (NIH3T3γ1). Auriculatin (1) and 8‐prenylluteone (2) showed inhibitory activity with an IC50 value of 20 μm on PLC in vitro as well as the formation of inositol phosphates in platelet‐derived growth factor (PDGF)‐stimulated NIH3T3γ1 cells. They also showed a moderate cytotoxicity against several human tumour cell lines with an IC50 of 9.0–20 μm, i.e. PC‐3 (prostate), NCI‐H226 (lung), CRL1579 (melanoma) in vitro. © 1998 John Wiley & Sons, Ltd.