2020
DOI: 10.21451/1984-3143-ar2019-0079 View full text |Buy / Rent full text
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Abstract: Although equine blastocysts ≤ 300 µm in diameter can be successfully vitrified, larger equine blastocysts are not good candidates for cryopreservation. As Na + , K + -ATPase is involved in maintaining blastocyst expansion, perhaps inhibition of this enzyme would be a viable method of reducing blastocyst diameter prior to cryopreservation. Objectives were to evaluate effects of ouabain-induced inhibition of Na + , K + -ATPase in equine blastocysts. Sixteen mares were ultrasonographically monitored, given deslor… Show more

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“…These ion channels such as NA + , K + , ATPase are essential for the accumulation of blastocoel and subsequently yolk sac fluid in equine embryos [ 92 ]. Their inhibition is detrimental for embryo development [ 93 ]. Thus, both reduced ATP production and disturbed ion channels’ function may impact embryo growth, although effects of mares’ age on embryo growth are controversial [ 24 28 ].…”
Section: Discussionmentioning
“…These ion channels such as NA + , K + , ATPase are essential for the accumulation of blastocoel and subsequently yolk sac fluid in equine embryos 86 . Their inhibition is detrimental for embryo development 87 . Thus, both reduced ATP production and disturbed ion channels’ function may impact embryo growth, although effects of mares’ age on embryo growth are controversial 2428 .…”
Section: Discussionmentioning
“…Their inhibition is detrimental for embryo development 87 . Thus, both reduced ATP production and disturbed ion channels' function may impact embryo growth, although effects of mares' age on embryo growth are controversial [24][25][26][27][28] .…”
Section: Discussionmentioning