Inhibition of miR-92b suppresses nonsmall cell lung cancer cells growth and motility by targeting RECK

Lin, Lei; Huang, Ya‐Ping; Gong, Wenrong

doi:10.1007/s11010-013-1882-5

Cited by 50 publications

(39 citation statements)

References 21 publications

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“…The possible regulatory mechanism of miR-21 can be showed in Fig. 9 [28], [29], [30], [31], [49], [50]. Furthermore, our data further underline the pivotal roles played by miR-21, provide new insight into the progression of squamous cell lung carcinoma, and also suggest that miR-21 may have potential diagnostic and therapeutic value for squamous cell lung carcinoma around the world.…”

Section: Discussionsupporting

confidence: 54%

“…Recently, several reports have demonstrated that PTEN is involved in cell apoptosis in human hepatocellular carcinoma cells [26] and also involved in cell proliferation, migration, and invasion in gastric cancer [27]. RECK is thought to be a tumor invasion and metastasis suppressor gene [28], and was also found to inhibit tumor cell growth and motility in both lung and bladder cancer [29], [30]. Bcl-2 is an oncogene and also a direct participant in the tumor cell apoptosis pathway [31].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-21 (miR-21) Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by Targeting PTEN, RECK and Bcl-2 in Lung Squamous Carcinoma, Gejiu City, China

et al. 2014

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BackgroundIn South China (Gejiu City, Yunnan Province), lung cancer incidence and associated mortality rate is the most prevalent and observed forms of cancer. Lung cancer in this area is called Gejiu squamous cell lung carcinoma (GSQCLC). Research has demonstrated that overexpression of miR-21 occurs in many cancers. However, the unique relationship between miR-21 and its target genes in GSQCLC has never been investigated. The molecular mechanism involved in GSQCLC must be compared to other non-small cell lung cancers in order to establish a relation and identify potential therapeutic targets.Methodology/Principal FindingsIn the current study, we initially found overexpression of miR-21 occurring in non-small cell lung cancer (NSCLC) cell lines when compared to the immortalized lung epithelial cell line BEAS-2B. We also demonstrated that high expression of miR-21 could increase tumor cell proliferation, invasion, viability, and migration in GSQCLC cell line (YTMLC-90) and NSCLC cell line (NCI-H157). Additionally, our results revealed that miR-21 could suppress YTMLC-90 and NCI-H157 cell apoptosis through arresting cell-cycle at G2/M phase. Furthermore, we demonstrated that PTEN, RECK and Bcl-2 are common target genes of miR-21 in NSCLC. Finally, our studies showed that down-regulation of miR-21 could lead to a significant increase in PTEN and RECK and decrease in Bcl-2 at the mRNA and protein level in YTMLC-90 and NCI-H157 cell lines. However, we have not observed any remarkable difference in the levels of miR-21 and its targets in YTMLC-90 cells when compared with NCI-H157 cells.Conclusions/SignificancemiR-21 simultaneously regulates multiple programs that enhance cell proliferation, apoptosis and tumor invasiveness by targeting PTEN, RECK and Bcl-2 in GSQCLC. Our results demonstrated that miR-21 may play a vital role in tumorigenesis and progression of lung squamous cell carcinoma and suppression of miR-21 may be a novel approach for the treatment of lung squamous cell carcinoma.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

MicroRNA-21 (miR-21) Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by Targeting PTEN, RECK and Bcl-2 in Lung Squamous Carcinoma, Gejiu City, China

et al. 2014

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“…For instance, miR-92b directly targets PTEN, promotes cell growth and induces cisplatin chemosensitivity in non-small cell lung cancer (NSCLC) cells (21). Inhibition of miR-92b suppresses NSCLC cell growth and motility by targeting RECK (22). Additionally, miR-92b functions as a potential oncogene in glioblastomas by targeting SMAD3 (23).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf‑related protein 3

Zhou

Feng

et al. 2017

Exp Ther Med

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Deregulation of microRNA-92b (miR-92b) has been implicated in osteosarcoma. However, the underlying regulatory mechanism of miR-92b in osteosarcoma growth and metastasis remains largely unclear. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were used to measure mRNA and protein expression. MTT and Transwell assays were conducted to determine cell proliferation and invasion, and a luciferase reporter assay was performed to confirm the association between miR-92b and Dickkopf3-related protein (DKK3). The results demonstrated that miR-92b was significantly upregulated in osteosarcoma tissues compared with matched adjacent non-tumor tissues. Additionally, high miR-92b levels were significantly associated with lung metastasis and advanced tumor, node, metastasis stage (P<0.05) but not with age, sex, tumor size, location, serum lactate dehydrogenase or serum alkaline phosphatase. miR-92b expression was also significantly upregulated in osteosarcoma cell lines compared with normal osteoblast cells. Knockdown of miR-92b significantly inhibited the proliferation and invasion of osteosarcoma U2OS cells (P<0.01). By contrast, overexpression of miR-92b significantly increased U2OS cell proliferation and invasion (P<0.01). DKK3 was identified as a target gene of miR-92b and it was demonstrated that DKK3 expression was negatively regulated by miR-92b in U2OS cells. Restoration of DKK3 expression abrogated the increased proliferation and invasion of U2OS cells induced by miR-92b overexpression. Notably, DKK3 was significantly downregulated in osteosarcoma tissues compared with adjacent non-tumor tissues and its expression was inversely correlated to miR-92b levels in osteosarcoma tissues. Taken together, these data indicate that miR-92b promotes cell proliferation and invasion in osteosarcoma by targeting DKK3. Therefore, miR-92b may become a potential therapeutic target for osteosarcoma.

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“…19, 20 In addition, acting as either tumor suppressors or oncogenes, miRNAs are involved in several aspects of cancer biology including cell proliferation, apoptosis, migration and invasion. 21, 22 In this study, we focused on miR-152 that belongs to the miR-148/152 family that includes miR-148a, miR-148b and miR-152. Many studies have revealed that members of the miR-148/152 family potentially act as oncogenes and tumor suppressors in different cancers.…”

Section: Discussionmentioning

confidence: 99%

MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2

Cheng

Tan

et al. 2014

Exp Mol Med

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MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

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Inhibition of miR-92b suppresses nonsmall cell lung cancer cells growth and motility by targeting RECK

Cited by 50 publications

References 21 publications

MicroRNA-21 (miR-21) Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by Targeting PTEN, RECK and Bcl-2 in Lung Squamous Carcinoma, Gejiu City, China

MicroRNA-21 (miR-21) Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by Targeting PTEN, RECK and Bcl-2 in Lung Squamous Carcinoma, Gejiu City, China

MicroRNA‑92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf‑related protein 3

MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2

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