Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma

Ma, Xinwei; Wang, Xiaoqi; Dong, Qian; Pang, Hongquan; Xu, Jianming; Shen, Jing; Zhu, Jianbing

doi:10.1186/s12935-021-01879-y

Cited by 13 publications

(14 citation statements)

References 25 publications

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“…6C&D). Previous study indicated that IRF6 mediated renal clear cell carcinoma cell apoptosis [34]. Consistently, we found that IRF6 also mediated the renal cell apoptosis (Fig.…”

Section: Discussionsupporting

confidence: 90%

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LncRNA ENSMUST_147219 mediated the progression of ischemic AKI via targeting of miR-221- 5p/IRF6 axis

Liu

Yang

et al. 2022

Preprint

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Although more studies revealed long noncoding RNAs (lncRNAs) regulated the progression of ischemic acute kidney injury(AKI), the exact role and mechanism of LncRNA ENSMUST_147219 in ischemic AKI was not unclear. In current study, LncRNA ENSMUST_147219 was induced by ischemic injury in vitro and vivo. Functionaly, LncRNA ENSMUST_147219 mediated the apoptosis in mouse proximal tubule-derived cell line(BUMPT) cells. Mechanistically, LncRNA ENSMUST_147219 sponged the microRNA (miR)-221-5p to upregulate the expression of Interferon Regulatory Factor 6 (IRF6) to drive apoptosis. Finally, knockdown of LncRNA ENSMUST_147219 markedly attenuated the ischemic AKI via targeting the miR-221-5p/IRF6 axis. Collectively, our data for the first time demonstrated that LncRNA ENSMUST_147219 promoted the development of ischemic AKI via regulation of miR-221-5p/IRF6 pathway, which could be considered as a new therapy target for ischemic AKI.

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“…6C&D). Previous study indicated that IRF6 mediated renal clear cell carcinoma cell apoptosis [34]. Consistently, we found that IRF6 also mediated the renal cell apoptosis (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…Previous studies reported that IRF6 mediated the Renal clear cell carcinoma cell apoptosis. [34]The prediction from the miRBase database indicated that miR-221-5p contained the complementary sequence of IRF6 (Fig. 6A).…”

Section: Irf6 Is a Direct Mir-221-5p Target Gene And Mediates I/r-ind...mentioning

confidence: 99%

LncRNA ENSMUST_147219 mediated the progression of ischemic AKI via targeting of miR-221- 5p/IRF6 axis

Liu

Yang

et al. 2022

Preprint

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“…GEPIA resource assembles TCGA and GTEx databases to focus on the expression of human genes and the correlation of the gene expression in multiple types of cancer; provides developmental information for evaluating the genes with aberrant expression in cancer. The GEPIA resource was highly cited in the previous studies [9][10][11], including ccRCC [12,13]. In the present study, we used the GEPIA resource to analyze the lncRNA that was most associated with the survival rates of patients with ccRCC and found PEBP1P2, whose expression was decreased in ccRCC and associated with the poor survival rates of ccRCC patients.…”

Section: Discussionmentioning

confidence: 92%

LncRNA PEBP1P2 Suppresses the Progression of Clear Cell Renal Cell Carcinoma Cells by Regulating the miR-27a-3p/PEBP1 Signal

Tang

et al. 2022

Preprint

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Background: Long noncoding RNA (lncRNA) PEBP1P2 has been shown to suppress the proliferation of vascular smooth muscle cells, but its role and underlying mechanisms in the development of clear cell renal cell carcinoma (ccRCC) remain largely unclear. Methods: Gene expression profiling interactive analysis (GEPIA) database was used to analyze the aberrant expression of lncRNAs in ccRCC tissues. Expression of RNAs and proteins was detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blotting, respectively. Ectopic expression of PEBP1P2 and miR-27a-3p was performed by pcDNA3.1 vector and miR-27a-3p mimics, respectively. Knockdown of PEBP1 mRNA was conducted by PEBP1 siRNA. Cell proliferation, migration, and invasion were analyzed using a CCK8 reagent and a trans-well assay, respectively. The sponge of PEBP1P2 to miR-27a-3p was investigated using Luciferase report system and RNA pull-down assays. Results: PEBP1P2 expression was decreased in ccRCC tissues compared with that in normal tissues and correlated with the size, TNM stage, and lymph node metastasis of the tumors, as well as the poor survival rates of ccRCC patients. Functionally, the enforced expression of PEBP1P2 inhibited the ability of ccRCC cells to proliferate, migrate, and invade in vitro . Mechanistically, PEBP1P2 suppressed the biological functions of ccRCC cells via increasing the mRNA and protein expression of PEBP1 and their expression had a positive correlation in ccRCC tissues. Furthermore, PEBP1P2 upregulated PEBP1 expression through acting as a competing endogenous RNA (ceRNA) to sponge miR-27a-3p, which directly target PEBP1 mRNA 3’UTR. Moreover, miR-27a-3p mimics rescued the decrease in the ability of ccRCC cells to proliferate, migrate, and invade mediated by PEBP1P2. Conclusion: Our findings indicated that PEBP1P2 suppressed ccRCC cell proliferation via regulating the miR-27a-3p/PEBP1 signal, suggesting that the PEBP1P2-miR-27a-3p/PEBP1 axis may be a potential therapeutic target for ccRCC.

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“…Previous studies have reported that IRF6 mediates renal clear cell carcinoma cell apoptosis [ 34 ]. The prediction from the miRBase database indicated that miR-221-5p contained the complementary sequence of IRF6 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…6 C and D). A previous study indicated that IRF6 mediated renal clear cell carcinoma cell apoptosis [ 34 ]. Consistently, we found that IRF6 mediated renal cell apoptosis (Fig.…”

Section: Discussionmentioning

confidence: 99%

LncRNA ENSMUST_147219 mediates the progression of ischemic acute kidney injury by targeting the miR-221-5p/IRF6 axis

Liu

Yang

et al. 2022

Apoptosis

View full text Add to dashboard Cite

Although previous studies have revealed that long noncoding RNAs (lncRNAs) regulate the progression of ischemic acute kidney injury (AKI), the exact role and mechanism of lncRNA ENSMUST_147219 in ischemic AKI are not clear. In the present study, lncRNA ENSMUST_147219 was induced by ischemic injury in vitro and in vivo. Functionally, lncRNA ENSMUST_147219 mediated apoptosis in mouse proximal tubule‐derived cell line (BUMPT). Mechanistically, lncRNA ENSMUST_147219 sponged the microRNA (miR)-221-5p to upregulate the expression of interferon regulatory factor 6 (IRF6) to drive apoptosis. Finally, knockdown of lncRNA ENSMUST_147219 markedly attenuated the ischemic AKI by targeting the miR-221-5p/IRF6 axis. Collectively, our data demonstrated that lncRNA ENSMUST_147219 promoted the development of ischemic AKI by regulating the miR-221-5p/IRF6 pathway, which could be considered a new therapeutic target for ischemic AKI.

show abstract

Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma

Cited by 13 publications

References 25 publications

LncRNA ENSMUST_147219 mediated the progression of ischemic AKI via targeting of miR-221- 5p/IRF6 axis

LncRNA ENSMUST_147219 mediated the progression of ischemic AKI via targeting of miR-221- 5p/IRF6 axis

LncRNA PEBP1P2 Suppresses the Progression of Clear Cell Renal Cell Carcinoma Cells by Regulating the miR-27a-3p/PEBP1 Signal

LncRNA ENSMUST_147219 mediates the progression of ischemic acute kidney injury by targeting the miR-221-5p/IRF6 axis

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