Inhibition of influenza virus fusion by polyanionic proteins

Isolation of a Novel Ubiquitin-like Protein from Pleurotus ostreatus Mushroom with Anti-Human Immunodeficiency Virus, Translation-Inhibitory, and Ribonuclease Activities

“…The reaction conditions were analogous to those described in the procedure of (17). Succinic anhydride (10 mg) was added to a solution of 10 mg of the protein in 10 ml 0.2 M K 2 HPO 4 (pH 8) and the solution was stirred until all anhydride had dissolved.…”

Antiviral properties of milk proteins and peptides

“…Kokuba, Aurelian, and Neurath (1998) reported that 3-HP-b-Lg prevented HSV-2 infection in the mouse model of genital HSV-2 infection, suggesting that 3-HP-b-Lg might be an efficacious agent for preventing vaginal transmission of genital herpesvirus infections. Schoen, Corver, Meijer, Wilschut, and Swart (1997) also reported that acylated milk proteins were effective against influenza virus.…”

Milk Derived Peptides with Immune Stimulating Antiviral Properties

“…Among the inhibitory proteins, 3-hydroxyphthalic anhydride -lactoglobulin also demonstrated a broad spectrum activity affecting herpes simplex virus 1 and 2 in addition to human cytomegalovirus by binding to the virus particles, inhibiting particularly the binding of monoclonal antibodies towards glycoprotein E and glycoprotein C [93]. Comparative results have been shown for anionic-modified human serum albumin and -lactoglobulin which prevents influenza virus membrane fusion with the host cell membrane, a process mediated by the viral glycoprotein hemagglutinin [94]. Interestingly, this anti-influenza effect has not been observed for other milk proteins carrying negative charges, like succinylated bovine serum albumin, lactalbumin, lactoferrin, lysozyme and transferrin [94] .…”

“…Comparative results have been shown for anionic-modified human serum albumin and -lactoglobulin which prevents influenza virus membrane fusion with the host cell membrane, a process mediated by the viral glycoprotein hemagglutinin [94]. Interestingly, this anti-influenza effect has not been observed for other milk proteins carrying negative charges, like succinylated bovine serum albumin, lactalbumin, lactoferrin, lysozyme and transferrin [94] . I t i s s a i d t h a t inhibition of viral fusion demonstrates a certain degree of specificity for negative charged proteins.…”

“…Binding to hemagglutinin of virus [36,53,135] modified human serum [94,[101][102][103] [12][13][14][15][16] chemically modified milk proteins like serum albumin, -lactalbumin, -lactoglobulin…”