Inhibition of green okra (Abelmoschus esculentus) extract against Enterococcus faecalis in tooth root canals

Abstract

Enterococcus faecalis is a bacterium that is often found in root canals of teeth and can survive in them even after treatment. The use of 2.5% NaOCl as a root canal irrigation material has disadvantages; toxic and irritating, so other alternatives are needed. Green okra extract contains flavonoids, alkaloids, saponins, tannins, and terpenoids which have antibacterial properties. This study aims to determine the inhibition of green okra extract against E.faecalis. It was doneexperimental laboratory research with a posttest-only control group design. The inhibition test used the disc diffusion method which consisted of 5 research groups,i.e. green okra extract with concentrations of 1.563%, 3.125%, 6.25%, 12.5%, and 2.5% NaOCl. Data were analyzed using non-pa-rametric statistical testsKruskal Wallis and Mann Whitney. The average of the inhibition zone diameter of the green okra extract concentrations were 1.563% (0 mm), 3.125% (0 mm), 6.25% (15.9 mm), 12.5% (18.03 mm), and 2.5% NaOCl (24.07 mm). The Kruskal Wallis test showed a significance value of 0.000 (p<0.05) which meant that there was a difference in the research group, followed by the Mann Whitney test which showed there were significant differences between all research groups except between green okra extract concentrations of 1.563% and 3.125%. It was concluded that green okra extract had an inhibitory effect on the growth of E.faecalis at concentrations of 6.25% and 12.5%.

Antibacterial ability of green okra fruit (Abelmoschus esculentus) extract at concentrations of 12.5-100% against Staphylococcus aureus

“…These various compounds can cause the extract's physical instability and may reduce its antibacterial ability. 20 The research on the antibacterial ability of green okra fruit was also performed on other bacteria found in the root canals of necrotic teeth, such as Enterococcus faecalis. 20 Elimination of the bacteria in the root canal affects the success of root canal treatment and prevents bacterial invasion of the periapical tissues.…”

“…20 The research on the antibacterial ability of green okra fruit was also performed on other bacteria found in the root canals of necrotic teeth, such as Enterococcus faecalis. 20 Elimination of the bacteria in the root canal affects the success of root canal treatment and prevents bacterial invasion of the periapical tissues. 21 Green okra fruit extract has demonstrated the ability to inhibit the growth of these bacteria.…”