Inhibition of complement activation by natural sulfated polysaccharides (fucans) from brown seaweed

Blondin, Catherine; Fischer, Elizabeth; Boisson-Vidal, Catherine; Kazatchkine, Michel D.; Jozefonvicz, J.

doi:10.1016/0161-5890(94)90121-x

Cited by 83 publications

(50 citation statements)

References 33 publications

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“…Zymosan produced intense inflammatory response with activation of the complement system and generation of chemotactic factors (Snyderman et al, 1970;Fernandez et al, 1978). The SP-Lv anti-edematogenic effect is in line with the in vitro studies showing inhibitory effects of sulfated fucans upon the complement system (Blondin et al, 1994;Tissot et al, 2003). The inhibitory action of SP-Lv upon plasma exudation was higher than its anti-edematogenic effect.…”

Section: Discussionsupporting

confidence: 72%

“…On the other hand, polymers of sulfated polysaccharides extracted from brown algae (fucans) showed potent inhibition on the human complement system in vitro (Blondin et al, 1994;Tissot et al, 2003). In this line, fucans of high molecular weight were able to inhibit the venular leukocyte rolling and endothelial lymphocyte adhesion induced by zymosan (Aruffo et al, 1991;Weston & Parish, 1991).…”

Section: Research Articlementioning

confidence: 99%

See 1 more Smart Citation

In vivoanti-inflammatory effect of a sulfated polysaccharide isolated from the marine brown algaeLobophora variegata

Siqueira

Silva

Alencar

et al. 2010

Pharmaceutical Biology

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Section: Discussionsupporting

confidence: 72%

Section: Research Articlementioning

confidence: 99%

In vivoanti-inflammatory effect of a sulfated polysaccharide isolated from the marine brown algaeLobophora variegata

Siqueira

Silva

Alencar

et al. 2010

Pharmaceutical Biology

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“…4,5) It was demonstrated that a sulfatedpolysaccharide fraction extracted from the brown algae Porphyra haitanesis presents an in vivo antioxidant property, causing an increase in the spleen and thymus size, suggestive of an immunostimulant action. 6) Additionally, sulfated galactans of the red marine algae Bryothamnion seaforthii presented antinociceptive activity in mice 7) and of the red micro algae Pophyridium sp.…”

mentioning

confidence: 99%

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Assreuy

Gomes

Silva

et al. 2008

Biological & Pharmaceutical Bulletin

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Sulfated-polysaccharides are complex macromolecular constituents of the extracellular matrix of marine algae that play an important role in mechanical, osmotic and ionic regulation.1) In Biomedicine the anticoagulant and antithrombotic properties have been most widely exploited and, at least for galactans, appear to be dependent on the sulfatation content and/or position of the sulfate groups.2) A natural sulfated polymer of L-fucose (fucoidan) showed a dose-dependent inhibition of leukocyte migration in the in vivo peritonitis model, 3) and sulfated fucans isolated from brown algae showed potent in vitro and in vivo inhibition of the human complement system. 4,5) It was demonstrated that a sulfatedpolysaccharide fraction extracted from the brown algae Porphyra haitanesis presents an in vivo antioxidant property, causing an increase in the spleen and thymus size, suggestive of an immunostimulant action. 6) Additionally, sulfated galactans of the red marine algae Bryothamnion seaforthii presented antinociceptive activity in mice 7) and of the red micro algae Pophyridium sp. anti-inflammatory action inhibiting eritema formation after topical use in humans.8) However, studies of sulfated galactans role in models of inflammation in vivo are rare in the literature. Here we isolated and investigated the effects of a sulfated galactan from the red marine algae Champia feldmannii, widely encountered along the South East and North East Brazilian sea coast, in experimental models of acute inflammation, coagulation and nociception. MATERIALS AND METHODS AnimalsMale Wistar rats (150-250 g) and Swiss mice (25-35 g) were maintained with a controlled 12/12 h light/dark cycle, at a temperature of 25°C with free access to food and water. The experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee of the State University of Ceará (UECE), Fortaleza-CE, Brazil, in accordance with international guidelines (NIH publication No. 85-23, revised 1985).Algae Champia feldmannii belongs to the order Rhodymeniales, family Lomentariaceae and was collected in the Pacheco beach of Caucaia, Ceará, Brazil. Algae were classified by Wladimir R. L. Farias, Department of Fishery Engeneering of Federal University of Ceará-Brazil.Erythrocytes Human erythrocytes were obtained from healthy donors at the Hematology Center of the Federal University of Ceará-Brazil.Drugs and Reagents Dextran sulphate, L-carrageenan, Evans blue, zymosan, morphine, cethylpiridinium chloride, sodium acetate, calcium chloride, ketamine, sulphuric acid, papain, 1-9-di-metylene blue (DMB), ethylenodiaminotetracetic acid (EDTA), indomethacin, dexamethasone, pentoxifylline, L-N-nitro-arginine methyl ester (L-NAME), meclyzine and formamide, N-acetyl-N,N,N-trimethylammonium bromide, 1,3-diaminopropane, toluidine blue, agarose gel (Sigma Chemical Co., St. Louis, MO, U.S.A. or SigmaAldrich Chemie, Steinheim, Germany); absolute ethanol, sodium chloride, sodium dodecyl sulfate (SDS) and 2-mer- 907, 60.455-970, Fortaleza-Ceará-Brasil. R...

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“…Fucoidans possess exceptional pharmaceutical activities such as anticoagulant [19], antibacterial and antiviral [20,21,22], antithrombotic [23], antitumoral [24], antiinflammatory [25], antiproliferative [17], and antifertilizing [18]. They are also successfully used for prevention and treatment of such widespread diseases as high blood pressure, arthritis, diabetes and obesity.…”

Section: Figmentioning

confidence: 99%

Studies on Fucoidan and Its Production From Vietnamese Brown Seaweeds

Lý¹,

Buu²,

Nhut³

et al. 2017

AJSTD

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Sulfated fucans are among the most widely studied of all the sulfated polysaccharides of plant origin that exhibit biological activities in mammalian systems. In this report fucoidans from some Vietnamese Sargassum species such as S. polycystum, S. oligocystum, S. mcclurei, S. swartzii and denticaprum were extracted and fractionated on a DEAE-Sephadex A-25 column.On the basis of chemical and spectral analyses, the fucoidan fractions obtained were found to be the sulfated fucogalactans containing sulfate ester groups and uronic acid, and composed essentially of fucose and galactose, as well as a minor amount of other sugars. The polysaccharide fractions were tested for anticancer activity. The primarily obtained results showed that all fucoidan fractions isolated from S. swartzii demonstrate bioactivity effects against cancer cells, while fraction F5 with a highest sulfate content exhibits the strongest antiinvasion activity. This indicates that sulfate content plays an important role in the anticancer activity of the brown algal fucoidans.A laboratory scale pilot for fucoidan production from Vietnamese brown seaweeds has been set with a capacity of 500 g of crude fucoidan per day.

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Inhibition of complement activation by natural sulfated polysaccharides (fucans) from brown seaweed

Cited by 83 publications

References 33 publications

In vivoanti-inflammatory effect of a sulfated polysaccharide isolated from the marine brown algaeLobophora variegata

In vivoanti-inflammatory effect of a sulfated polysaccharide isolated from the marine brown algaeLobophora variegata

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Studies on Fucoidan and Its Production From Vietnamese Brown Seaweeds

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