Inhibition of Candida albicans attachment to extracellular matrix by antibodies which recognize hydrophobic cell wall proteins

Masuoka, J

doi:10.1016/s0928-8244(99)00059-0

Cited by 17 publications

(38 citation statements)

References 34 publications

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“…The yeast isolates used in this study are listed in Table 1. Isolates were grown and subsequently subcultured (inoculum concentration of 1 ϫ 10 6 to 3 ϫ 10 6 cells/ml) in Sabouraud dextrose broth (SDB) or phosphate-buffered yeast nitrogen base supplemented with 2% glucose (YNB2G) at 23 or 37°C to stationary phase (26 to 28 h) (29,43). All assays were performed on cells which were harvested from the second subculture (third pass in the indicated medium).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis

Hazen

Wu²,

Masuoka³

2001

Infect Immun

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Although Candida dubliniensis is a close genetic relative of Candida albicans, it colonizes and infects fewer sites. Nearly all instances of candidiasis caused by C. dubliniensis are restricted to the oral cavity. As cell surface hydrophobicity (CSH) influences virulence of C. albicans, CSH properties of C. dubliniensis were investigated and compared to C. albicans. Growth temperature is one factor which affects the CSH status of stationaryphase C. albicans. However, C. dubliniensis, similar to other pathogenic non-albicans species of Candida, was hydrophobic regardless of growth temperature. For all Candida species tested in this study (C. albicans, C. dubliniensis, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis), CSH status correlated with coaggregation with the anaerobic oral bacterium Fusobacterium nucleatum. Previous studies have shown that CSH status of C. albicans involves multiple surface proteins and surface protein N-glycans. The hydrophobic surface glycoprotein CAgp38 appears to be expressed by C. albicans constitutively regardless of growth temperature and medium. C. dubliniensis expresses a 38-kDa protein that cross-reacts with the anti-CAgp38 monoclonal antibody; however, expression of the protein was growth medium and growth temperature dependent. The anti-CAgp38 monoclonal antibody has been shown to inhibit adhesion of C. albicans to extracellular matrix proteins and to vascular endothelial cells. Since protein glycosylation influences the CSH status of C. albicans, we compared the cell wall mannoprotein content and composition between C. albicans and C. dubliniensis. Similar bulk compositional levels of hexose, phosphate, and protein in their N-glycans were determined. However, a component of the C. albicans N-glycan, acid-labile phosphooligomannoside, is expressed much less or negligibly by C. dubliniensis, and when present, the oligomannosides are predominantly less than five mannose residues in length. In addition, the acid-labile phosphooligomannoside profiles varied among the three strains of C. dubliniensis we tested, indicating the N-glycan of C. dubliniensis differs from C. albicans. For C. albicans, the acid-labile phosphooligomannoside influences virulence and surface fibrillar conformation, which affects exposure of hydrophobic surface proteins. Given the combined role in C. albicans of expression of specific surface hydrophobic proteins in pathogenesis and of surface protein glycosylation on exposure of the proteins, the lack of these virulence-associated CSH entities in C. dubliniensis could contribute to its limited ability to cause disseminated infections.

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Section: Methodsmentioning

confidence: 99%

Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis

Hazen

Wu²,

Masuoka³

2001

Infect Immun

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“…This could be a factor in the high virulence of hydrophobic cells compared to that of hydrophilic cells in mice. MAbs to cell surface hydrophobic protein inhibited the adherence of cells to fibronectin and laminin (237). Pretreatment of hydrophobic cells with MAb also reduced adherence to endothelial cell monolayers (125).…”

Section: Adhesinsmentioning

confidence: 96%

Candida albicansCell Wall Proteins

Chaffin

2008

Microbiol Mol Biol Rev

425

404

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SUMMARY The Candida albicans cell wall maintains the structural integrity of the organism in addtion to providing a physical contact interface with the environment. The major components of the cell wall are fibrillar polysaccharides and proteins. The proteins of the cell wall are the focus of this review. Three classes of proteins are present in the candidal cell wall. One group of proteins attach to the cell wall via a glycophosphatidylinositol remnant or by an alkali-labile linkage. A second group of proteins with N-terminal signal sequences but no covalent attachment sequences are secreted by the classical secretory pathway. These proteins may end up in the cell wall or in the extracellular space. The third group of proteins lack a secretory signal, and the pathway(s) by which they become associated with the surface is unknown. Potential constituents of the first two classes have been predicted from analysis of genome sequences. Experimental analyses have identified members of all three classes. Some members of each class selected for consideration of confirmed or proposed function, phenotypic analysis of a mutant, and regulation by growth conditions and transcription factors are discussed in more detail.

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Section: Introductionmentioning

confidence: 99%

“…Some authors have reported that antibody immunity against C. albicans may participate in host defence by preventing attachment (Han & Cutler, 1995 concerned the inhibition of attachment by antibodies that recognize cell-wall proteins (Masuoka et al, 1999;San Millan et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The role of antibody immunity in fungal infections remains a controversial subject. Many in vivo or in vitro studies have provided evidence for or against the importance of antibody immunity to C. albicans (Casadevall, 1995 concerned the inhibition of attachment by antibodies that recognize cell-wall proteins (Masuoka et al, 1999;San Millan et al, 1996).We study here the adherence of C. albicans to polystyrene and to ECM components after incubation of the yeast with IgG directed against cytoplasmic antigens of C. albicans, compared with its adherence after contact of the yeast with nonspecific IgG. …”

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confidence: 99%

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Immunoglobulins G could prevent adherence of Candida albicans to polystyrene and extracellular matrix components

Rodier¹,

Imbert²,

Kauffmann‐Lacroix³

et al. 2003

Journal of Medical Microbiology

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Immunocompromised patients are at high risk of developing Candida infections. Although cellmediated immunity is generally believed to play the main role in defence against fungi, antibodies could also be effective in immune defence by different mechanisms of action. The adherence capacity of four strains of Candida albicans to polystyrene and to some extracellular matrix components was investigated after incubation of the yeasts with non-specific and specific anti-C. albicans IgG. Experiments were carried out using a colorimetric method based upon the reduction of XTT tetrazolium (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) by mitochondrially active blastospores in the presence of menadione. Incubation of the yeasts with IgG, specific or not, caused a decrease in the capacity for adherence to the surfaces studied. There was no significant effect of the specificity of the tested antibodies on the reduction of adherence capacity. In conclusion, total IgG could play a role in blocking the binding of C. albicans to host and medical device surfaces. These results suggest that regular survey of levels of total IgG in patients suffering from severe hypogammaglobulinaemia could be of interest for the prevention of systemic candidiasis. INTRODUCTIONCandida albicans, a saprophyte of the human digestive tract, is frequently responsible for systemic infections in immunocompromised patients, even if other species of Candida are reported with increased frequency (Vincent et al., 1998). According to a wide-ranging American study, the rate of invasive fungal infections among hospital patients approximately doubled between 1980 and 1990, and the incidence of nosocomial candidaemia alone increased fivefold (BeckSagué & Jarvis, 1993).C. albicans possesses virulence factors that are required for the establishment of candidiasis, involved in processes such as adhesion, phenotypic switching and morphogenesis (Calderone & Fonzi, 2001). Adhesion of the organism to mucosal epithelium is a prerequisite for colonization and is therefore regarded as the initial step in the process leading to infection. Furthermore, adhesion to endothelium and extracellular matrix (ECM) components are required for dissemination of C. albicans (Klotz, 1992). A number of ECM proteins bind to the yeast, including fibronectin (Klotz et al., 1994), laminin (Sakata et al., 1999), vitronectin and type I and IV collagens (Klotz et al., 1993). Moreover, C. albicans is also able to adhere to the surface of intravascular catheters, usually colonized by intra-or extraluminal migration of Candida spp. from the skin surface (Flanagan & Barnes, 1998). Candidaemia occurs commonly in the presence of a colonized intravascular catheter (Rex, 1996).Nevertheless, the ability of this yeast to cause human infectious disease relates more to the immunological status of the host than to obvious virulence factors produced by the fungus. C. albicans is an opportunist yeast that becomes a pathogen in hosts whose local or systemic immune functions are impair...

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Inhibition of Candida albicans attachment to extracellular matrix by antibodies which recognize hydrophobic cell wall proteins

Cited by 17 publications

References 34 publications

Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis

Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis

Candida albicansCell Wall Proteins

Immunoglobulins G could prevent adherence of Candida albicans to polystyrene and extracellular matrix components

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