Inhibition of CA V decreases glucose synthesis from pyruvate

Dodgson, Susanna J.; Forster, R. E.

doi:10.1016/0003-9861(86)90066-4

Cited by 59 publications

(41 citation statements)

References 14 publications

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“…In higher vertebrates, seven a-CA isoenzymes have been described. One of these, CA V, is located in the mitochondria and has been proposed to supply HCO1 to the urea cycle and to gluconeogenesis (6,7).…”

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confidence: 99%

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Eriksson

Karlsson

Ramazanov

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

159

131

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confidence: 99%

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Eriksson

Karlsson

Ramazanov

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

159

131

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“…Previously Acetazolamide and Ethoxyzolamide were found to inhibit gluconeogenesis in vitro or after acute administration in rats. [18][19][20] our studies indicate that Acetazolamide treatment in STZ induced diabetic rats' lowers glucose production and increases glycolytic production of lactate. We found (Table 2) 6.9 fold increase in blood lactate level when compared with normal control and 3.0 fold increases in blood lactate level when compared with untreated diabetic group.…”

Section: Carbonic Anhydrase Inhibition (With Acetazolamide) Increasesmentioning

confidence: 84%

Inhibition of Carbonic Anhydrase Results in Blood Lactate Accumulation (BLA) in STZ Induced Diabetic Rats

Ismail¹,

Ameh²,

Amodu³

et al. 2015

J Diabetes Metab

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Carbonic anhydrase is a pH regulatory enzyme, whose inhibition leads to metabolic acidosis. We investigated whether carbonic anhydrase inhibition may be associated with blood lactate accumulation in Streptozotocin (STZ) induced diabetic rats. The study aimed to provide a new marker to assist in identifying diabetic individuals at a high risk of developing lactic acidosis. Erythrocyte carbonic anhydrase activity with 4-nitrophenyl acetate as substrates was investigated in (STZ) induced diabetic rats. STZ induced diabetic rats showed significant increase in erythrocyte carbonic anhydrase (CA) activity of 9.7 ± 0.7 μMol/min/μL and blood lactate level of 6.2 ± 1.2 mMol/L when compared with Non Diabetic group CA enzyme activity of 5.5 ± 0.6 μMol/min/μL and blood lactate level of 3.9 ± 0.5 mMol/L. Inhibition of erythrocyte carbonic anhydrase activity with Acetazolamide results in 6.9 fold increase in blood lactate level when compared with Non diabetic group. Therefore inhibition of Carbonic anhydrase in STZ induced diabetic rats' result in blood lactate accumulation and reduced blood glucose concentration.

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“…Previous published studies have provided mixed results regarding the potential of carbonic anhydrase inhibitors to modulate blood glucose levels. Acetazolamide and ethoxyzolamide were found to inhibit gluconeogenesis in vitro or after acute administration in rats [28][29][30]. [28] concluded that carbonic anhydrase is functionally important for gluconeogenesis in the male guinea pig liver when there is a requirement for bicarbonate as substrate.…”

Section: Discussionmentioning

confidence: 99%

“…Acetazolamide and ethoxyzolamide were found to inhibit gluconeogenesis in vitro or after acute administration in rats [28][29][30]. [28] concluded that carbonic anhydrase is functionally important for gluconeogenesis in the male guinea pig liver when there is a requirement for bicarbonate as substrate. We may therefore suggest that carbonic anhydrase plays a crucial role in gluconeogenesis, due to the fact that carbonic anhydrase inhibition does not only lowers blood glucose level but increases blood lactate level as well.…”

Section: Discussionmentioning

confidence: 99%