Inherited predisposition to CLL is detectable as subclinical monoclonal B-lymphocyte expansion

Non‐CLL‐like monoclonal B‐cell lymphocytosis in the general population: Prevalence and phenotypic/genetic characteristics

“…However, it should be noted that within our non-CLL-like MBL cases, both CD5 þ and CD5 À cases were included; by contrast, in previous reports, only CD5 À cases were usually considered, whereas most CD5 þ cases which did not show a typical CLL-like phenotype were classified as atypical CLL-like Bcell clones, even when only a limited panel of antibodies was used to define their immunophenotype (6,9). In fact, if we only consider CD5 À non-CLL-like MBL cases, their frequency in our series is similar to that previously reported by others (1.4% vs. between 1% and 1.4% (6,7,9,12)), even when we used a flow cytometric approach associated with a higher sensitivity as regards detection of CLL like B-cell clones (10). On the basis of these results, our findings would support the hypothesis that the screening approach used could be associated with a lower sensitivity as regards detection of non-CLL versus CLL-like MBL clones in PB samples from healthy subjects who show normal lymphocyte counts.…”

“…In line with these results, previous reports on MBL cases associated with normal (7-10) and increased (2-6) PB lymphocyte counts, also indicate that in most CD5 À non-CLL-MBL cases, clonal B-cells display either an unclassifiable or MZL-like immunophenotype. In addition, we also confirm that among MBL cases detected in the general population, the presence of CD10 þ B-cell clones is rather infrequent, if at all detected (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In contrast, here we describe for the first time, an MBL case which shows both phenotypic and genetic features that are highly characteristic of MCL cells.…”

“…Until now, non-CLL like MBL has been arbitrarily divided into two major groups: CD5 þ and CD5 À MBL cases. Although in several reports specific reference is made to the phenotypic and even the cytogenetic characteristics of some MBL cases presenting with lymphocytosis (4,11), little information is provided about non-CLL like B-cell clones in subjects who have MBL and normal Bcell counts (6,7,9,12,13). In addition, limited data exists concerning the outcome of subjects with non-CLL-like MBL either detected in population screening studies (9,13) or during investigation for lymphocytosis (2,4,11).…”

Germline copy number variation associated with Mendelian inheritance of CLL in two families

“…The affected family includes a father with CLL (generation 1) and his four children, three of whom also developed CLL (generation 2, Figure 1a). The three unaffected children of individual A-2A were assessed for evidence of monoclonal B cell lymphocytosis (MBL) 4 with four-color flow cytometry and PCR to detect monoclonal IgH rearrangement (Supplementary Figure 2). These individuals had no evidence of MBL as yet but are young.…”

Proliferação monoclonal B CD5+ subclínica

“…Os estudos de parentes sadios de primeiro grau de pacientes pertencentes a estas famílias mostraram que a freqüência de LBM era maior do que a detectada na população em geral. Um estudo 11,12 que analisou 59 indiví-duos sadios, advindos de 21 famílias, encontrou oito indivíduos (13,5%) com células semelhantes às encontradas na doença. Em outra publicação 13 foram estudados 33 indivíduos pertencentes a nove famílias com LLC e encontradas células B monoclonais em seis (18%).…”