Inheritance and fitness costs of <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae) resistance to spinosad in Brazil

Okuma, Daniela M.; Bernardi, Daniel; Horikoshi, Renato J.; Bernardi, Oderlei; Silva, Aline P; Omoto, Celso

doi:10.1002/ps.4829

Cited by 98 publications

(95 citation statements)

References 54 publications

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“…the resistance loci are in autosomal chromosomes. As in most cases of insecticide resistance, other studies have reported autosomal resistance of S. frugiperda , to lambda‐cyhalothrin, carbaryl, lufenuron and spinosad . For the diamide insecticides, the resistance of P. xylostella and T. absoluta was characterized as autosomal and incompletely recessive dominance …”

Section: Discussionmentioning

confidence: 99%

“…As in most cases of insecticide resistance, other studies have reported autosomal resistance of S. frugiperda, to lambda-cyhalothrin, 25 carbaryl, 23 lufenuron and spinosad. 28,29 For the diamide insecticides, the resistance of P. xylostella and T. absoluta was characterized as autosomal and incompletely recessive dominance. 54,55 Dominance of resistance under field conditions depends on many factors including the concentration applied, the development stage of the insect, and environmental factors.…”

Section: Discussionmentioning

confidence: 99%

“…19 Chemical control has been one of the main management strategies for S. frugiperda, but this armyworm has developed resistance to the main chemical groups including pyrethroids (lambda-cyhalothrin), carbamates (carbaryl) and organophosphates (chlorpyriphos), as well as more recently developed chemical groups such as benzoylurea (lufenuron) and spinosyn (spinosad). [20][21][22][23][24][25][26][27][28][29] In addition, due to the wide adoption of transgenic plants expressing Bacillus thuringiensis Berliner proteins (Bt plants), cases of S. frugiperda resistance to Bt proteins are already reported in Brazil. [30][31][32] Because they are still efficacious, 6,8 diamide insecticides are an important component of the management of this pest in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Bolzan

Padovez

Nascimento

et al. 2019

Pest Management Science

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139

137

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BACKGROUND Understanding the genetic basis of insect resistance to insecticides can help to implement insecticide resistance management (IRM) strategies. In this study, we selected a strain of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistant to chlorantraniliprole using the F2 screen method, characterized the inheritance of resistance and evaluated patterns of cross‐resistance to other diamide insecticides. RESULTS The chlorantraniliprole‐resistant strain (Chlorant‐R) was selected from a field‐collected population with an estimated allele frequency of 0.1316. The estimated median lethal concentration (LC50) values were 0.011 and 2.610 µg a.i. cm–2 for the susceptible (Sus) and Chlorant‐R strains, respectively, resulting in a resistance ratio (RR) of 237‐fold. The LC50 values of the reciprocal crosses were 0.155 and 0.164 µg a.i. cm–2, indicating that resistance is autosomally inherited. Resistance was characterized as incompletely recessive and monogenic at concentrations close to the recommended field rates of chlorantraniliprole. Survival of the resistant strain and heterozygous larvae in maize plants treated at the field rate was ∼ 60 (similar to the susceptible strain on untreated maize) and 15%, respectively. The Chlorant‐R strain showed low cross‐resistance to cyantraniliprole (RR ∼ 27‐fold) and very high cross‐resistance to flubendiamide (RR > 42 000‐fold). CONCLUSIONS The frequency of chlorantraniliprole resistance allele was moderately high in a field‐collected population of S. frugiperda. The inheritance of chlorantraniliprole resistance was characterized as autosomal, incompletely recessive and monogenic. S. frugiperda showed cross‐resistance to other diamide insecticides. These results provide essential information for the implementation of IRM programs to preserve the useful life of diamide insecticides for controlling S. frugiperda in Brazil. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Bolzan

Padovez

Nascimento

et al. 2019

Pest Management Science

Self Cite

139

137

View full text Add to dashboard Cite

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“…Because of the evolution of resistance of FAW to some Bt proteins expressed in corn in Brazil, the use of chemical insecticides has increased in some corn‐producing regions . However, frequent reports of resistance to major chemical insecticides such as spinosad, lambda ‐cyhalothrin, chlorpyrifos and lufenuron, challenges the control of this pest. Thus, the number of effective modes of action available to implement resistance management strategies, is shrinking.…”

Section: Discussionmentioning

confidence: 99%

“…Such a treatment frequency is conducive to the development of insecticide resistance in FAW, so it is not surprising that high levels of resistance to several chemical classes of insecticides have been reported recently in Puerto Rico . In Brazilian populations of FAW, resistance to a range of synthetic insecticides, addressing different modes of action, including organophosphates, carbamates, pyrethroids, benzoylureas and spinosyns, has been also described …”

Section: Introductionmentioning

confidence: 99%

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Boaventura

Bolzan

Padovez

et al. 2019

Pest Management Science

Self Cite

112

113

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BACKGROUND Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), a major lepidopteran pest in Latin and North America, has very recently invaded the continents of Africa and Asia. FAW has evolved resistance to different insecticides and transgenic corn expressing Bacillus thuringiensis (Bt) toxins. Here, we investigated the extent and mechanisms of resistance to diamide insecticides in a Brazilian field‐collected FAW strain selected using chlorantraniliprole. RESULTS Continuous laboratory selection of a field‐collected FAW strain with chlorantraniliprole resulted in resistance ratios of 225‐fold and > 5400‐fold against chlorantraniliprole and flubendiamide, respectively, when compared with a susceptible strain. Pre‐exposure to different synergists known to inhibit detoxification enzymes did not result in significantly increased larval toxicity, suggesting a minor role for metabolic resistance. Sequencing of the FAW ryanodine receptor (RyR) C‐terminal domains II to VI revealed a single nucleotide polymorphism, resulting in a I4734M mutation recently said to confer target‐site resistance to diamides in lepidopteran pests. Genotyping by pyrosequencing of field‐collected FAW larvae sampled in the 2018 crop season suggests a low resistance allele frequency. Furthermore, we developed a fluorescent polymerase chain reaction (PCR)‐based allelic discrimination assay for rapid genotyping of field‐collected FAW samples, because diamides are increasingly used in Bt−/non‐Bt corn. CONCLUSIONS Recently, the identified RyR mutation has been shown to confer field resistance in other lepidopteran pests such as diamondback moth, tomato leafminer and striped rice stem borer. The developed PCR‐based allelic discrimination assay will help to monitor the frequency and future spread of diamide resistance allele in FAW field populations and help to implement appropriate resistance management measures. © 2019 Society of Chemical Industry

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Optimal rotation of insecticides to prevent the evolution of resistance in a structured environment

Yamamura

2021

Population Ecology

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Several strategies have been used in insecticide resistance management to prevent the evolution of resistance, but the spatial aspects of insecticide application are crucially important among these strategies. Here, we consider a structured environment that consists of on‐farm and off‐farm fields where crops are planted periodically in on‐farm fields during cultivation periods. We define the basic reproduction rate (R0) of resistance as the expected number of offspring of a resistant individual divided by that of a susceptible individual under the condition that the proportion of resistance is extremely small; it is measured as the quantity per cycle of the cultivation period. We calculate loge()R0 using realistic dose‐survival curves under a given fitness cost of resistance genes. The evolution of resistance occurs if and only if the loge()R0 value is larger than 0. Then, we propose a procedure for calculating the optimal design of rotational spraying that prevents the evolution of resistance, that is, the evolutionary stable strategy (ESS) for farmers, satisfying the mortality required for managing the abundance of insects. We consider the following controllable factors in calculating the optimal design: the dose of insecticide, the number of sprays, the number of different types of insecticides and potentially, the size of on‐farm fields.

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Inheritance and fitness costs of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to spinosad in Brazil

Cited by 98 publications

References 54 publications

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Optimal rotation of insecticides to prevent the evolution of resistance in a structured environment

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