Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium

Lee, So Yeong; Yoon, Kyoungro; Jang, Seung‐Ho; Ganbold, Erdene‐Ochir; Dembereldorj, Uuriintuya; Shin, Sang Mo; Ryu, Pan Dong; Joo, Sang Woo

doi:10.4142/jvs.2009.10.4.299

Cited by 31 publications

(14 citation statements)

References 24 publications

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“…Majority (80%) of the changes in the band position could have resulted from stretching vibrations of C = O group while a small impact could also be due to stretching vibrations of C-N group and bending vibrations of the N-H group in the β-sheet structure of proteins [39]. Peaks at~1535 cm -1 , which correspond to amide II, may be largely due to the coupling of CN stretching and in-plane bending of the N-H group [46]. The changes in the hydrogen bonding of the peptide group and consequently in the molecular geometry of the proteins may instigate cell damages in the protein folding, resulting in a definitive loss of protein biological functions or mutations [24].…”

Section: Discussionmentioning

confidence: 99%

ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer

et al. 2020

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Lung cancer remains the leading cause of cancer-related death worldwide. Since prognosis and treatment outcomes rely on fast and accurate diagnosis, there is a need for more costeffective, sensitive, and specific method for lung cancer detection. Thus, this study aimed to determine the ability of ATR-FTIR in discriminating malignant from benign lung tissues and evaluate its concordance with H&E staining. Three (3) 5μm-thick sections were cut from formalin fixed paraffin embedded (FFPE) cell or tissue blocks from patients with lung lesions. The outer sections were H&E-stained and sent to two (2) pathologists to confirm the histopathologic diagnosis. The inner section was deparaffinized by standard xylene method and then subjected to ATR-FTIR analysis. Distinct spectral profiles that distinguished (p<0.05) one sample from another, called the "fingerprint region", were observed in five (5) peak patterns representing the amides, lipids, and nucleic acids. Principal component analysis and hierarchical cluster analysis evidently clustered the benign from malignant tissues. ATR-FTIR showed 97.73% sensitivity, 92.45% specificity, 94.85% accuracy, 91.49% positive predictive value and 98.00% negative predictive value in discriminating benign from malignant lung tissue. Further, strong agreement was observed between histopathologic readings and ATR-FTIR analysis. This study shows the potential of ATR-FTIR spectroscopy as a potential adjunct method to the gold standard, the microscopic examination of hematoxylin and eosin (H&E)-stained tissues, in diagnosing lung cancer.

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Section: Discussionmentioning

confidence: 99%

ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer

et al. 2020

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“…8 Both optical spectroscopy methods have shown a great potential in the discrimination of malignant and benign tissue and have been applied in colorectal, breast, prostate, and lung cancer research. [10][11][12][13] To further investigate the diagnostic potential of fluorescence and nearinfrared spectroscopy and taking the need for novel tools in CRC diagnostics into account, the discrimination of malignant and benign colorectal tissue was assessed using fluorescence and near-infrared spectroscopy in an ex vivo setting.…”

Section: Introductionmentioning

confidence: 99%

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Ehlen

Zabaryło

Speichinger

et al. 2019

Journal of Surgical Research

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Magnetic resonance spectroscopy a b s t r a c tBackground: Colorectal cancer is one of the most common malignancies worldwide. There is an urgent need for simple and fast methods to improve tumor detection in the diagnostic and intraoperative setting to avoid complications and provide objective information in distinguishing malignant and benign colorectal tissue. Optical spectroscopy methods have recently shown a great potential for this discrimination in different organs. Materials and methods:In this pilot study, fluorescence emission spectra (excitation: 473 nm) and diffuse reflectance spectra (DRS) of normal and tumor tissues from resected colorectal cancer specimen were measured using fiber optical probes in an ex vivo setting, and the data were subjected to multivariate analysis.Results: Substantial spectral differences were found in the fluorescence and DRS spectra of colorectal cancer tissue in comparison to benign tissue. The diagnostic potential of a multimode optical system combining both spectroscopic methods was investigated by mathematical combination. Compared with the individual techniques, a higher sensitivity of the joint DRS-fluorescence optical system in the discrimination between malignant and benign colorectal tissue could be observed. Conclusions:In the pilot study presented herein, a quick and reliable method to differentiate malignant and benign colorectal tissue ex vivo with different spectroscopic techniques using spectral fiber probes could be established. Joint fluorescence and near-infrared spectroscopy had a higher sensitivity in tissue discrimination and showed to be a promising combination of two spectroscopic methods. Further studies using the synergic effect of fluorescence and DRS spectroscopy are needed to transfer these findings into the in vivo situation. ª

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“…4,7–15 By directly comparing a biological state with spectroscopic data, a chemical signature of the disease process may be possible. Since IR spectra are holistically reflective of chemical composition, spectroscopic measurements can potentially yield more information than observing individual molecular targets.…”

Section: Introductionmentioning

confidence: 99%

Subcellular localization of early biochemical transformations in cancer-activated fibroblasts using infrared spectroscopic imaging

Holton

Walsh

Bhargava

2011

Analyst

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The tumor microenvironment, or stroma, is chemically and morphologically modified during carcinoma progression. The predominant cell type in the stroma, the fibroblast, maintains collagen properties in normal tissue and often transformed during tumor progression. Biochemical changes within fibroblasts upon initial cancer activation, however, are relatively poorly defined. Here, we hypothesized that Fourier transform infrared (FT-IR) spectroscopic imaging could potentially be employed to examine these early transformations. Further, we employ attenuated total reflectance (ATR) microscopy to characterize subcellular spectra and their changes upon transformation. We characterized fibroblast transitions upon stimulation with both a molecular agent and a carcinoma-mimicking cellular co-culture system. Changes were predominantly observed in the 1080 cm−1 and 1224 cm−1 peak absorbance, commonly associated with nucleic acids, as well as in the band at 2930 cm−1 associated with the C-H stretching of proteins in the cytoplasmic compartment. In conclusion, biochemical changes in cancer-associated fibroblasts that express a-SMA are dominated by the cytoplasm, rather than the nucleus. This ensures that spectral changes are not associated with proliferation or cell cycle processes of the cells and the cells are undergoing a true phenotypic change denoted by protein modifications in the cell body.

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Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium

Cited by 31 publications

References 24 publications

ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer

ATR-FTIR spectroscopy as adjunct method to the microscopic examination of hematoxylin and eosin-stained tissues in diagnosing lung cancer

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Subcellular localization of early biochemical transformations in cancer-activated fibroblasts using infrared spectroscopic imaging

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