Infrared and nuclear magnetic resonance studies of some surface properties of asbestos–albumin interactions

Dumitru-Stanescu, R.; Mandravel, Cristina; Bercu, C.

doi:10.1039/an9941900689

Cited by 12 publications

(3 citation statements)

References 7 publications

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“…[28] In the last decades, the adsorption of albumin onto mineral asbestos fibers has been investigated by employing X-ray photoelectron spectroscopy (XPS), [29] FTIR and NMR spectrometries, [30] revealing the tendency of mineral chrysotile fibers to yield adducts with albumin. These studies have dealt mainly with electrostatic interactions between the asbestos surface and albumin, focussing on the different behaviors of chrysotile and crocidolite.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Bovine Serum Albumin with Chrysotile: Spectroscopic and Morphological Studies

Falini

Foresti

Lesci

et al. 2006

Chemistry A European J

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The biodurability of chrysotile fibers, which is related to their cytotoxicity and mutagenic responses, is strongly affected by the surface chemical adsorption of biological molecules. Natural chrysotile is a heterogeneous material in both structure and composition. The availability of synthetic stoichiometric chrysotile of constant structure and uniform morphology has allowed us to investigate its interaction with bovine serum albumin (BSA). By using transmission electron microscopy (TEM) and atomic force microscopy (AFM), we have obtained the first morphological evidence of albumin adsorption onto chrysotile nanocrystals. FTIR spectroscopy was used to quantify modifications of BSA secondary structure that were induced by the surface interaction. The protein transition to beta-turns allows a stronger interaction between the protein hydrophilic side-chains and the charged asbestos surface, which is consistent with hydrogen bonds involving the superficial OH groups. Synthetic stoichiometric chrysotile nanocrystals were shown to be an ideal reference standard with which to study the interaction of asbestos fibers with biological systems, in order to elucidate the chemical mechanisms of asbestos toxicity.

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Section: Introductionmentioning

confidence: 99%

Interaction of Bovine Serum Albumin with Chrysotile: Spectroscopic and Morphological Studies

Falini

Foresti

Lesci

et al. 2006

Chemistry A European J

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“…Chemical modification by derivitizing the surfaces of mineral fibre silicate has revealed alterations in reactivity in model in vitro cell toxicity studies, suggesting a multifactorial character of particle-cell interactions (Evans et al 1983). The adsorption of bovine serum albumin onto asbestos fibres has been shown by infrared spectroscopy and nmr to be mediated by 0 -H-N hydrogen bonds (Dumitru- Stanescu et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Surface chemistry and biological pathogenicity of silicates: an X-ray photoelectron spectroscopic study

Seal

Krezoski

Barr

et al. 1996

Proc. R. Soc. Lond. B

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We extend our electron spectroscopy for chemical analysis studies of the chemistry of silicates to provide direct surface chemical information on the interactions involved in silicate-induced lung and tissue pathology. A total of five fibrous and non-fibrous silicate minerals, primarily amphiboles, have been studied: anthophyllite, tremolite, cummingtonite, hornblende and actinolite. We have followed the 'inlattice' surface chemistry of these materials and monitored features such as the simultaneous presence of four- and six-coordinate (with respect to oxygen) structural aluminium, and the presence of iron in the M4 octahedral positions. In vitro experiments involving contact of the silicate with cultured murine Ehrlich cells have identified modifications in the surface chemistry of Al, Mg and Fe in the silicates and changes in cellular iron content.

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“…It has been used to identify types of asbestos, 18 to gain information about the cation distribution between coordination sites 12 and to examine interactions between asbestos and protein. 19 The idealised amphibole asbestos structure is composed of double chains of linked silica tetrahedra, which form a silica layer. These layers are cross linked with seven bridging cations as can be seen in figure 1.…”

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confidence: 99%

Chemical differences between long and short amosite asbestos: differences in oxidation state and coordination sites of iron, detected by infrared spectroscopy.

Graham

Higinbotham²,

Allan³

et al. 1999

Occup Environ Med

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Objectives-Short fibres of amosite asbestos (SFA), obtained by ball milling of long fibres (LFA), have been shown to be less pathogenic than long fibres. Accumulating evidence suggests an important role for diVerences in surface chemistry between fibres. Iron has been implicated in the pathogenesis of asbestos fibres. In this study infrared (IR) spectroscopy was used to compare LFA and SFA in terms of the coordination and oxidation state of iron at the three cation sites (M 1 , M 3 , M 1 ). Methods-Infrared was used to examine LFA ad SFA, when dry and when hydrated in the presence and absence of the chelators desferroxamine and ferrozine. With appropriate software the proportions of iron and its oxidation states in the overlapping peaks were resolved and assigned, and the three coordination sites were identified. Data were obtained from 10 samples of both lengths of fibre for each of the four treatments. Iron release was also monitored. Results-Iron was significantly more oxidised in LFA than SFA. Further oxidation of the dry fibres with water, ferrozine, or desferroxamine tended to abolish these diVerences. There were also significant diVerences between the proportions of iron held in the diVerent coordination sites of the fibres. For LFA, a higher proportion of its iron was held in the cation sites coordinating less with iron and more with Mg. Interestingly, the sites coordinating single irons were significantly more oxidised than multiple sites. The single iron sites were more oxidised in LFA than SFA and were more readily oxidised by the treatments. Conclusions-Important chemical diVerences between LFA and SFA were found. There seemed to be some mobility of iron near the surface. Based on these data it is speculated that the 1 iron surface site may be important in pathogenesis. (Occup Environ Med 1999;56:606-611)

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Infrared and nuclear magnetic resonance studies of some surface properties of asbestos–albumin interactions

Cited by 12 publications

References 7 publications

Interaction of Bovine Serum Albumin with Chrysotile: Spectroscopic and Morphological Studies

Interaction of Bovine Serum Albumin with Chrysotile: Spectroscopic and Morphological Studies

Surface chemistry and biological pathogenicity of silicates: an X-ray photoelectron spectroscopic study

Chemical differences between long and short amosite asbestos: differences in oxidation state and coordination sites of iron, detected by infrared spectroscopy.

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