Infochemicals in Mosquito Host Selection: Human Skin Microflora and Plasmodium Parasites

Braks, Marieta; Anderson, Robert A.; Knols, Bart G. J.

doi:10.1016/s0169-4758(99)01514-8

Cited by 79 publications

(53 citation statements)

References 35 publications

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“…A high concentration was found to be repellent. Contrary to what has been found for other mosquito species, no synergistic effect of CO 2 in combination with skin emanations has been observed (Allan et al 2006b, Braks et al 1999.…”

Section: Culex Quinquefasciatuscontrasting

confidence: 76%

“…gambiae is strongly attracted to human emanations, in particular skin emanations. A single finger (Dekker et al 2001b, Smallegange et al 2002, ethanol washings of human hands and feet (Braks 1999), human sweat Takken 1999, Meijerink et al 2000, Smallegange et al in press), and skin emanations of human hands or feet transferred to fabric (Dekker et al 2001a, Pates et al 2001b, Qiu et al 2004b, Smallegange et al in press, Spitzen et al 2008 or glass beads (Qiu et al 2004a) are highly attractive when tested in wind tunnels, Y-tube or dual-choice olfactometers or indoor experiments with mosquito traps.…”

Section: Anopheles Gambiae Ssmentioning

confidence: 99%

“…quinquefasciatus females from a colony that originated from Sri Lanka that were released individually in a dual-port olfactometer were attracted within three minutes to polyamide stockings that had been worn by a human volunteer for the preceding 48-60 hours , whereas, in a separate study, less than 25% of the mosquitoes from a colony in the USA released in groups of 50-70 females were attracted to a human hand (Allan et al 2006b). Skin rubbings and ethanol washings of human hands and feet or the back were attractive to groups or individual female mosquitoes (Braks et al 1999). These results demonstrate that Cx.…”

Section: Culex Quinquefasciatusmentioning

confidence: 99%

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Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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Section: Culex Quinquefasciatuscontrasting

confidence: 76%

Section: Anopheles Gambiae Ssmentioning

confidence: 99%

Section: Culex Quinquefasciatusmentioning

confidence: 99%

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Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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“…Studies with An. gambiae s.s. have been more successful; this species is attracted to carboxylic acids ) NH 3 , (Braks et al, 1999, and to chemical compounds present in incubated human sweat but which are absent from freshly collected sweat . No studies on the effects of odor blends with these mosquito species have as yet been published although one study (Mboera et al, 2000) reported the attraction of Cx.…”

Section: Behavioral Responses To Single Compounds and Odor Blendsmentioning

confidence: 99%

Olfactory regulation of mosquito–host interactions

Zwiebel

Takken

2004

Insect Biochemistry and Molecular Biology

271

165

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Mosquitoes that act as disease vectors rely upon olfactory cues to direct several important behaviors that are fundamentally involved in establishing their overall vectorial capacity. Of these, the propensity to select humans for blood feeding is arguably the most important of these olfactory driven behaviors in so far as it significantly contributes to the ability of these mosquitoes to transmit pathogens that cause diseases such as dengue, yellow fever and most significantly human malaria. Here, we review significant advances in behavioral, physiological and molecular investigations into mosquito host preference, with a particular emphasis on studies that have emerged in the post-genomic era that seek to combine these approaches.

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“…Recently it was demonstrated that the An. gambiae OBP1 is required for in vivo responses to indole and 3-methyl indole (31), both of which are major components of incubated human sweat that is attractive to female mosquitoes (3)(4)(5)(6). Additional studies have proposed that this response requires the formation of heterodimeric complexes between OBP1 and OBP4 (32,33).…”

mentioning

confidence: 99%

New Insights into the Mechanism of Odorant Detection by the Malaria-transmitting Mosquito Anopheles gambiae

Davrazou

Dong

Murphy

et al. 2011

Journal of Biological Chemistry

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Anopheles gambiae mosquitoes that transmit Plasmodium falciparum malaria use a series of olfactory cues present in human sweat to locate their hosts for a blood meal. Recognition of these odor cues occurs through the interplay of odorant receptors and odorant-binding proteins (OBPs) that bind to odorant molecules and transport and present them to the receptors. Recent studies have implicated potential heterodimeric interactions between two OBPs, OBP1 and OBP4, as important for perception of indole by the mosquito (Biessmann, H., Andronopoulou, E., Biessmann, M. R., Douris, V., Dimitratos, S. D., Eliopoulos, E., Guerin, P. M., Iatrou, K., Justice, R. W., Kröber, T., Marinotti, O., Tsitoura, P., Woods, D. F., and Walter, M. F. (2010) PLoS ONE 5, e9471; Qiao, H., He, X., Schymura, D., Ban, L., Field, L., Dani, F. R., Michelucci, E., Caputo, B., della Torre, A., Iatrou, K., Zhou, J. J., Krieger, J., and Pelosi, P. (2011) Cell. Mol. Life Sci. 68, 1799 -1813). Here we present the 2.0 Å crystal structure of the OBP4-indole complex, which adopts a classical odorant-binding protein fold, with indole bound at one end of a central hydrophobic cavity. Solution-based NMR studies reveal that OBP4 exists in a molten globule state and binding of indole induces a dramatic conformational shift to a well ordered structure, and this leads to the formation of the binding site for OBP1. Analysis of the OBP4-OBP1 interaction reveals a network of contacts between residues in the OBP1 binding site and the core of the protein and suggests how the interaction of the two proteins can alter the binding affinity for ligands. These studies provide evidence that conformational ordering plays a key role in regulating heteromeric interactions between OBPs.Anopheles gambiae mosquitoes are the primary vectors for malaria caused by Plasmodium falciparum and have an extremely high preference for feeding on human hosts (1, 2) and are attracted to odor molecules from incubated human sweat and other skin emanations (3-6). Disrupting the normal olfactory responses to these odors presents an attractive tool to combat transmission of malaria and other mosquito borne diseases, and a number of efforts are now under way to discover novel reagents for this purpose.In insects the detection of odorants occurs primarily in the olfactory sensilla and involves the interplay of membranebound olfactory receptors (7) and odorant-binding proteins (OBPs), 3 which are expressed into the lymph fluid that surrounds the olfactory dendrites (8) where they can reach concentrations in the millimolar range (9, 10). OBPs have multiple roles including protecting odors from degradation and transporting them to the olfactory receptors (11, 12). There is evidence that OBPs have two primary roles in odorant perception. In the first model a number of groups have proposed that OBPs act as passive carriers for the odorant, and pH changes in the vicinity of the dendritic membrane lead to conformational changes that stimulate ligand release, freeing the ligand to activate the receptor (1...

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Infochemicals in Mosquito Host Selection: Human Skin Microflora and Plasmodium Parasites

Cited by 79 publications

References 35 publications

Olfaction in vector-host interactions

Olfaction in vector-host interactions

Olfactory regulation of mosquito–host interactions

New Insights into the Mechanism of Odorant Detection by the Malaria-transmitting Mosquito Anopheles gambiae

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