Influences of Centrifugation on Cells and Tissues in Liposuction Aspirates: Optimized Centrifugation for Lipotransfer and Cell Isolation

Abstract

Excessive centrifugation can destroy adipocytes and adipose-derived stem cells, but appropriate centrifugation concentrates them, resulting in enhanced graft take. The authors tentatively recommend 1200 g as an optimized centrifugal force for obtaining good short- and long-term results in adipose transplantation.

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

“…However, like aspiration pressure, the physical effects of centrifugation may have an effect on SVF viability and the number of ASCs available for culture. A study by Kurita and colleagues 31 has shown that speeds in excess of 3,000 x g can damage the ASC. However, speeds should be significant enough to ensure adequate pelleting of the SVF.…”

Adipose-Derived Stem Cells for Regenerative Medicine

“…43 The recovery of ASCs can be improved further by manipulating the centrifugation speed. 45 Investigators have achieved optimal cell recovery using a centrifugation speed of 1200g based on the subsequent formation of a human-derived adipose tissue depot following implantation in an immunodeficient murine model. 45 The cell isolation process requires the manipulation of large volumes of lipid-laden cells, presenting potential risks to equipment and personnel.…”

“…45 Investigators have achieved optimal cell recovery using a centrifugation speed of 1200g based on the subsequent formation of a human-derived adipose tissue depot following implantation in an immunodeficient murine model. 45 The cell isolation process requires the manipulation of large volumes of lipid-laden cells, presenting potential risks to equipment and personnel. To facilitate the process, several groups have fabricated devices to automate the cell isolation.…”

Autologous Fat Transplantation – A Paradigm Shift in Breast Reconstruction

“…The cells may be damaged due high G-forces (Kurita et al, 2008)  It is a time and labor-consuming process  High fat concentrations may cause clumping and more difficult flow during re-injection One of the most confusing metrics in fat grafting is a lack of standardization when one discusses "percent yields". Once fat is lipo-aspirated as donor graft there are an infinite number of different concentrations of adipocyte volume relative to non-adipocyte volume (blood, serum, crystalloid) that can be reached prior to grafting the recipient site.…”