Influences of aberration on spatial resolution of STED microscope in probing a specimenwith discontinuous refraction indices

Xiao, Chao; Zhang, Chen; Zhu, Jianing; Zhao, Wei; Bai, Jintao; He, Qian; Wang, Kaige

doi:10.1364/ao.58.002112

Cited by 2 publications

(1 citation statement)

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“…10,11 Later, based on vectorial Debye theory, Gu et al theoretically studied the expression of the electric field and aberration of light passing through a vertical dielectric surface. 12 The shaping, shifting and stretching of linearly polarised beam focused through the interfaces between different mediums, such as air-silicon, 10 air-glass, 13 glass-water 14 and air-birefringent crystal 15,16 have been investigated in detail. The characteristics of tightly converging cylindrically polarised beams 15,[17][18][19] and Bessel-modulated beams 18 through dielectric interfaces are systematically studied as well.…”

Section: Introductionmentioning

confidence: 99%

Aberration on excitation focal spot caused by oblique interface with refractive indices discontinuous and its correction with pure‐phase compensation for laser scanning microscopy

Zhu

Zhang

Zhao

et al. 2021

Journal of Microscopy

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The interface of mediums with refractive indices discontinuous, for example airglass and glass-water, are inevitable in microscopic imaging. In this work, the aberration of oblique interface with refractive index discontinuous on the laser scanning microscope was investigated theoretically with numerical simulations. It was found that the position, shape and FWHM of focal spots, were all significantly affected by the aberration due to oblique interface. The aberration can cause serious shifting of focal spots in the axial direction of beam during -scanning and lead to an inaccurate reconstruction of three-dimensional (3D)targets. The aberration can also lead to a decreasing spatial resolution. To correct the influence of the aberration, a pure-phase modulation method has been proposed. By applying a phase compensation map into a spatial light modulator (SLM), the oblique interface aberration had been corrected experimentally in a laser scanning microscope. We hope this research can attract the attention of researchers when using scanning microscope, especially for reconstructing 3D biological and material structures.

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