Influence on mitogen-activated protein kinases as a new direction of connective tissue growth regulation

Shurygina, I. A.; Шурыгин, М. Г.; Зеленин, В. Н.; Аюшинова, Н. И.

doi:10.20538/1682-0363-2017-4-86-93

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…MAPKs constitute a protein family relaying signal transduction, amplification and integration and thus providing for appropriate physiological responses of mammalian cells, such as proliferation, differentiation, inflammation and apoptosis. (21)(22)(23)(24) One of the MAP kinase subfamilies, p38 MAPK, is known to be activated under stress conditions caused by ultraviolet radiation, heat shock, osmotic stress, lipopolysaccharide, protein synthesis inhibitors, pro-inflammatory cytokines (IL-1, TNF-α, etc. ), and also by certain mitogens realizing the effect through tyrosine kinase receptors in particular.…”

Section: Discussionmentioning

confidence: 99%

Changes in Oxidative Phosphorylation Activity in Fibroblasts at p38 MAPK Pathway Inhibition

Shurygina¹,

Trukhan²,

Дремина³

et al. 2019

IJBM

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Background: Mitochondrial oxidative phosphorylation (OxPhos) accounts for more than 90% of the cellular ATP production, plays the role in reactive oxygen species (ROS) generation and programmed cell death. In addition, it contributes to such cellular processes as proliferation, differentiation and cell aging. Currently, several signaling systems are known to participate in regulation of OxPhos and activity of cytochrome c-oxidase (CcO), the terminal enzyme of the mitochondrial electron transport chain. However, data on mechanisms and key units involved in the signal transduction are still being supplemented. Methods: Peritoneal fibroblasts were isolated from the omentum of Wistar rats by fragmenting the dissected tissue and disaggregating the fragments in collagenase solution (200 U/ml). The primary culture of fibroblasts was cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% Fetal Bovine Serum (FBS), 1% antibiotic/antimycotic at 37°C, 80% RH and 5% СО 2 in Biostation CT, Nikon. To obtain a culture, the fibroblasts were subcultured every 7 days. After the third passage the culture was treated with SB203580 at the concentration of 10 μM or with SB203580 in combination with bFGF at the dose of 133 pg/ml. Cells for immunofluorescent studies were fixed with 70% ethanol and stained with antibodies to CcO subunit I. Results: When exposed to SB203580 or the combination of SB203580 and bFGF, marked changes were observed in the fibroblast culture: in both cases there was intensive collagen destruction; attached fibroblasts rounded and detached from the substrate. When exposed to SB203580, non-rounded cells started to vacuolate actively while vacuoles occupied the entire cytoplasm. Introduction of the p38 inhibitor into the culture of activated fibroblasts caused a more intensive cell detachment and collagen destruction. Moreover, the formation of large conglomerates containing several dozens of cells connected with collagen fibers was observed in the areas characterized by the highest cell density. Immunofluorescent staining made it possible to reveal a certain increase in the cell area occupied by CcO after fibroblasts exposure to SB203580 and a significant increase in the area of the enzyme distribution in the studied cells (more than 5-fold in comparison with the control group) when simultaneously adding SB203580 and bFGF. In addition, one-way ANOVA test demonstrated a statistically significant increase in the CcO fluorescent staining intensity in both cases. Conclusion: The analysis of the results indicates that SB203580, a p38 MAPK inhibitor, influences both peritoneal fibroblast morphology and the energy status of the cells under study increasing the amount of CcO, the terminal enzyme of the mitochondrial electron transport chain, in the cells, although no cell change to active proliferation or apoptosis was observed. Fibroblast culture stimulation by bFGF significantly enhances the effect of SB203580, which implies a greater OxPhos complex expression in case of impaired p38 MAPK signaling pathway ...

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Section: Discussionmentioning

confidence: 99%

Changes in Oxidative Phosphorylation Activity in Fibroblasts at p38 MAPK Pathway Inhibition

Shurygina¹,

Trukhan²,

Дремина³

et al. 2019

IJBM

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“…The process of dormant cells' converting into cells allowing the ECM production, is called fibroblast activation [15]. Depending on their location in organs, tissues and functional activity, fibroblasts produce collagen and elastin subproteins, such as procollagen, proelastin, fibronectin, laminin, and tenascin [16][17][18][19][20]. There are several fibroblast cell types: fibroblasts, myofibroblasts, fibrocytes fibroclasts, progenitor cells, and poorly differentiated [16].…”

Section: Introductionmentioning

confidence: 99%

“…Depending on their location in organs, tissues and functional activity, fibroblasts produce collagen and elastin subproteins, such as procollagen, proelastin, fibronectin, laminin, and tenascin [16][17][18][19][20]. There are several fibroblast cell types: fibroblasts, myofibroblasts, fibrocytes fibroclasts, progenitor cells, and poorly differentiated [16]. All of the presented cell types can be found in the skin.…”

Section: Introductionmentioning

confidence: 99%

Review of optical methods for noninvasive imaging of skin fibroblasts—From in vitro to ex vivo and in vivo visualization

Nikolaev,

Kistenev,

Kröger

et al. 2024

Journal of Biophotonics

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Fibroblasts are among the most common cell types in the stroma responsible for creating and maintaining the structural organization of the extracellular matrix (ECM) in the dermis, skin regeneration, and a range of immune responses. Until now, the processes of fibroblast adaptation and functioning in a varying environment have not been fully understood. Modern laser microscopes are capable of studying fibroblasts in vitro and ex vivo. One‐photon‐ and two‐photon‐excited fluorescence microscopy, Raman spectroscopy/micro‐spectroscopy are well‐suited non‐invasive optical methods for fibroblast imaging in vitro and ex vivo. In vivo staining‐free fibroblast imaging is not still implemented. The exception is fibroblast imaging in tattooed skin. Although in vivo non‐invasive staining‐free imaging of fibroblasts in the skin has not yet been implemented, it is expected in the future. This review summarizes the state of the art in fibroblast visualization using optical methods and discusses the advantages, limitations, and prospects for future non‐invasive imaging.This article is protected by copyright. All rights reserved.

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Selenium Nanoparticles

Shurygina

Trukhan

Дремина

et al. 2021

Nanotechnology in Medicine

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Influence on mitogen-activated protein kinases as a new direction of connective tissue growth regulation

Cited by 7 publications

References 23 publications

Changes in Oxidative Phosphorylation Activity in Fibroblasts at p38 MAPK Pathway Inhibition

Changes in Oxidative Phosphorylation Activity in Fibroblasts at p38 MAPK Pathway Inhibition

Review of optical methods for noninvasive imaging of skin fibroblasts—From in vitro to ex vivo and in vivo visualization

Selenium Nanoparticles

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