Influence of the Temperature on Coccolith‐Containing Systems from<i>Emiliania huxleyi</i>Cultivations

Chairopoulou, Makrina A.; Kratzer, Fabian; Groß, Roland; Herrmann, Michael; Teipel, Ulrich

doi:10.1002/ceat.201900507

Cited by 7 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The temperature increase induces the entropy of the molecules, reducing their binding ability, which supports the dissolution of the oily and proteinaceous binders from the inorganic components. The temperature with which we are working had no visible impact on the nannofossils, partially corroborating the work of Chairopoulou [106] even though the study of Coto [107] showed an increasing solubility under certain conditions already from 20 °C on the contrary. This procedure alone does not work, but helps the solvent mix with the organic particles, breaks the bonds of the molecules, and reduces the time of disaggregation.…”

Section: Interaction Temperature-chalksupporting

confidence: 86%

Novel nannofossils extraction methods from paintings, coupled with GC–MS for provenance determination and binder analysis

et al. 2022

View full text Add to dashboard Cite

The use of calcareous nannofossils for provenance analysis is a new-old topic for cultural heritage. Several studies have already mentioned it for ceramic, but less for paintings. Preparatory layers of the paintings are often made with chalk, which is composed of microfossils. To extract a calcareous nannofossils assemblage from a painting layer, we need to disaggregate it. The method is to plunge the micro-samples into water and heat it if water alone does not work. The disaggregation process takes a long time and is not efficient in terms of quantitative results. In this work, we aimed to develop a disaggregation method that increases the number of determinable nannofossils extracted from a painting micro-sample. As these samples are valuable and unique, we decided that a combination of analyses on the disaggregated micro-sample should be tried to extract the most information from it. We studied the possibility of binder determination by gas chromatography–mass spectrometer after the nannofossils assemblage extraction on the residual liquid from the disaggregation. The method we are presenting is easy to apply, has a high disaggregation rate for most paintings, and a low impact on binders fatty acids for their determination.

show abstract

Section: Interaction Temperature-chalksupporting

confidence: 86%

Novel nannofossils extraction methods from paintings, coupled with GC–MS for provenance determination and binder analysis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The temperature rises induce the entropy of the molecules reducing their binding ability, which helps the dissolution of the oily and proteinaceous binders from the inorganic components. The temperature with which we are working had no visible impact on the coccoliths partially corroborating the work of Chairopoulou (92) even though the study of Coto (93) showed an increasing solubility under certain conditions already from 20°C on the contrary. This procedure alone does not work but helps the solvent to mix with the organic particles, breaks the molecules bonds and reduces the time of disaggregation.…”

Section: Interaction Temperature-chalksupporting

confidence: 85%

Novel nannofossils extraction methodology from paintings coupled with GC-MS for provenance determination and binder analysis

Jaques

Trubač

Rathouský

et al. 2022

Preprint

View full text Add to dashboard Cite

Calcareous nannofossils for provenance analysis is a new-old topic for cultural heritage. Several studies already mentioned it for ceramic but less for paintings. Preparatory layers of the paintings are often made with chalk which is composed of microfossils. In order to extract the calcareous nannofossils assemblage from these layers, we need to disaggregate it. Actually, the method is to plunge the micro-samples into the water and heat it if the water alone does not work. The resulting disaggregation takes long and is not efficient in terms of quantitative results or time. In this work, we aimed to develop a disaggregation method that will extract the most nannofossils from the preparatory layer with the least destruction. As these samples are unique and we should make the most of them, we observe if gas chromatography-mass spectrometer analysis could be done on the disaggregated sample in order to determine the binders after the nannofossils retrieval. The method we are presenting is relatively easy to use, can be applied for most paintings and have a low impact on binders for their determination.

show abstract

“…A similar content was obtained for the two methods (48.0% by TG versus 50.8% by muffle furnace) with the advantages of the TG method, which requires only a few milligrams of biomass and is more automated than the muffle method. The high ash content of cultured E. huxleyi when compared with non-coccolithophore microalgae [ 53 ] can be due to the presence of calcium carbonate, as its decomposition is expected above 600 °C [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The Polar Lipidome of Cultured Emiliania huxleyi: A Source of Bioactive Lipids with Relevance for Biotechnological Applications

et al. 2020

View full text Add to dashboard Cite

Polar lipids from microalgae have aroused greater interest as a natural source of omega-3 (n-3) polyunsaturated fatty acids (PUFA), an alternative to fish, but also as bioactive compounds with multiple applications. The present study aims to characterize the polar lipid profile of cultured microalga Emiliania huxleyi using hydrophilic interaction liquid chromatography coupled with high-resolution mass spectrometry (HILIC–MS) and fatty acids (FA) analysis by gas chromatography (GC–MS). The lipidome of E. huxleyi revealed the presence of distinct n-3 PUFA (40% of total FA), namely docosahexaenoic acid (22:6n-3) and stearidonic acid (18:4n-3), which give this microalga an increased commercial value as a source of n-3 PUFA present in the form of polar lipids. A total of 134 species of polar lipids were identified and some of these species, particularly glycolipids, have already been reported for their bioactive properties. Among betaine lipids, the diacylglyceryl carboxyhydroxymethylcholine (DGCC) class is the least reported in microalgae. For the first time, monomethylphosphatidylethanolamine (MMPE) has been found in the lipidome of E. huxleyi. Overall, this study highlights the potential of E. huxleyi as a sustainable source of high-value polar lipids that can be exploited for different applications, namely human and animal nutrition, cosmetics, and pharmaceuticals.

show abstract

Influence of the Temperature on Coccolith‐Containing Systems fromEmiliania huxleyiCultivations

Cited by 7 publications

References 27 publications

Novel nannofossils extraction methods from paintings, coupled with GC–MS for provenance determination and binder analysis

Novel nannofossils extraction methods from paintings, coupled with GC–MS for provenance determination and binder analysis

Novel nannofossils extraction methodology from paintings coupled with GC-MS for provenance determination and binder analysis

The Polar Lipidome of Cultured Emiliania huxleyi: A Source of Bioactive Lipids with Relevance for Biotechnological Applications

Contact Info

Product

Resources

About