Influence of the functional activating agent on the biochemical and kinetic properties of Candida rugosa lipase immobilized on chemically modified cellulignin

Characterization of Candida rugosa Lipase Immobilized on Poly(N‐methylolacrylamide) and Its Application in Butyl Butyrate Synthesis

“…The values of conversion and productivity obtained using the immobilized system were higher than those previously related to this enzyme in the free form to catalyze butyl butyrate synthesis (about 40 %) [7], confirming the protection of the catalyst due to immobilization.…”

“…Thus, exploiting good immobilizing carriers has been an attractive work for enzyme engineering [6]. Although there are many studies on different carriers to immobilize lipase, development efforts continue to focus on new options [7,8] because there is no perfect carrier and immobilization method for all processes. Moreover, the choice of an enzyme carrier for a specific process depends not only on technical aspects but also on availability and economic analysis.…”

Covalent attachment of Candida rugosa lipase on chemically modified hybrid matrix of polysiloxane–polyvinyl alcohol with different activating compounds

“…Therefore, the evaluation of other activating agents of the support could be useful to enhance the catalytic activities of the immobilized derivatives. Among the available activating agents, sodium metaperiodate (META) and carbonyldiimidazole (CDI) have been already demonstrated satisfactory performance to activate somewhat different support which can be used as matrix for lipase immobilization [17,18].…”

Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

“…Lowcost support materials are preferred for industrial applications; they can be organic chitin, chitosan or inorganic CaCO 3 26 . The organic support materials could come from lignocellulosic agro-industrial wastes, such as sawdust, wood chips/shavings, straw, spent grains 27 , rice straw 26,28 , cellulignin 29 , rice husk 30 , or coconut fiber 31 .…”