Influence of temperature and storage duration on measurement of activated partial thromboplastin time, D‐dimers, fibrinogen, prothrombin time and thrombin time, in citrate‐anticoagulated whole blood specimens

Zhao, Ying; Lv, Guoliang

doi:10.1111/ijlh.12113

Cited by 33 publications

(56 citation statements)

References 11 publications

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“…Although many domestic and foreign scholars have studied the effects of pre-analytical variables on coagulation test analysis, no studies have described the stability of FVIII:C and FIX:C at 25°C and 4°C. A summary of these studies is listed in Table 328101213151617. Nonetheless, there are no unified guidelines for clinically acceptable bias.…”

Section: Discussionmentioning

confidence: 99%

“…The results following storage for 2, 4, 6, 8, 12, and 24 h at 25°C and 4°C were compared with the baseline results by using paired t-tests. To assess the stability of the coagulation tests and factor activities, the percentage changes compared to the baseline results were calculated [(result at storage time X – result at baseline)/result at baseline] and averaged for each time point2121314. According to the study by van Geest-Daalderop et al, a clinically relevant difference was defined as a mean percentage change of greater than 10%.…”

Section: Methodsmentioning

confidence: 99%

“…Pre-analytical variables including specimen collection, anticoagulant type and concentration, hematocrit, filling status of the sampling tube, transportation, centrifugation, as well as storage and assay method can all affect coagulation test and factor analysis results12. Activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT), international normalized ratio (INR, transformed by PT), and thrombin time (TT) measurements are routine coagulation tests used to assess pathological alterations of hemostatic and coagulation systems to guide clinical therapy2. In addition, the PT/INR ratio is used to monitor oral anticoagulant therapy for reducing the risk of thromboembolic events and minimizing the incidence of bleeding complications3.…”

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confidence: 99%

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Effects of storage time and temperature on coagulation tests and factors in fresh plasma

Lin

Zhao

et al. 2014

Sci Rep

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Coagulation tests and factors measurements have been widely applied in clinical practice. Pre-analytical conditions are very important in laboratory assessment.Here,we aim to determine the effects of storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT), the international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in fresh plasma. Seventy-two blood samples were tested after storage for 0 (baseline), 2, 4, 6, 8, 12, and 24 h at 25°C (room temperature) and 4°C (refrigeration) in two centers. The mean percentage change of greater than 10% and the numbers of samples with greater than 10% percentage changes more than 25% were used to determine clinically relevant difference. We demonstrated that samples for Fbg, PT/INR, and TT could be safely stored for ≤24 h; FVIII:C for ≤2 h; FIX:C for ≤4 h both at 4°C and 25°C; and APTT for ≤12 h at 4°C and ≤8 h at 25°C.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Effects of storage time and temperature on coagulation tests and factors in fresh plasma

Lin

Zhao

et al. 2014

Sci Rep

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“…Zhao and Lv [25] examined the influence of temperature and storage duration on the measurements in routine coagulation tests. Their work is similar to our study in that we examined the same test parameters using citrate anticoagulated specimens.…”

Section: Discussionmentioning

confidence: 99%

Effect of Centrifuge Temperature on Routine Coagulation Tests

2018

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Background: This study investigated the effects of cooled and standard centrifuges on the results of coagulation tests to examine the effects of centrifugation temperature. Methods: Equal-volume blood samples from each patient were collected at the same time intervals and subjected to standard (25°C) and cooled centrifugation (2–4°C). Subsequently, the prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer values were determined in runs with the same lot numbers in the same coagulation device using the Dia-PT R (PT and INR), Dia-PTT-liquid (aPTT), Dia-FIB (fibrinogen), and Dia-D-dimer kits, respectively. Results: The study enrolled 771 participants. The PT was significantly (p < 0.018) higher in participants on anticoagulant therapy. The respective median values of the test parameters determined using the standard and cooled centrifuges were as follows: PT 10.30 versus 10.50 s; PT (INR) 1.04 versus 1.09 s; APTT 28.90 versus 29.40 s; fibrinogen 321.5 versus 322.1 mg/dL; and D-dimer 179.5 versus 168.7 µg FEU/mL. There were significant differences (p < 0.001) in the parameters between the values obtained with the standard and cooled centrifuges. Conclusions: Centrifuge temperature can have a significant effect on the results of coagulation tests. However, broad and specific disease-based studies are needed.

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“…Pre-analytical variables including specimen collection, storage, temperature, transport, anticoagulant type, haematocrit, filling status of the sampling tube and centrifugation variable can potentially affect analysis results and by extension the medical care offered to patients [2]. Prothrombin time (PT), international normalized ratio (INR) and the Activated partial thromboplastin time (APTT are common and readily available coagulation tests used to investigate the pathological changes to the haemostatic and coagulation systems and to monitor clinical therapy particularly among patient on anticoagulant therapy (warfarin and Heparin), patients with thromboembolic events, haemorrhage and to monitor coagulopathy [2]. Prothrombin Time (PT) measures the integrity of the extrinsic system as well as factors common to both systems and Partial Thromboplastin Time (PTT) measures the integrity of the intrinsic system.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Time and Temperature Variables on Some Routine Coagulation Tests among Subjects of African Descent in Sokoto, North Western Nigeria

Ikhuenbor¹,

Aghedo²,

Isah³

et al. 2016

OJBD

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This study evaluated the effects of time and temperature variables on routine Prothrombin Time test and Activated Partial Thromboplastin Time (APTT) test among subjects of African descent in Sokoto, North Western Nigeria. Samples of 99 subjects made up of 49 male and 50 female subjects with mean age 38.3 ± 22.3 years. Coagulation tests were performed immediately specified times after phlebotomy up to 24 hours (0, 1, 2, 3, 4 and 24 hours at room temperature of 40 degrees C. Our data demonstrate that prothrombin time and APTT results are stable for up to 2 hours, remaining constant regardless of storage conditions. Post hoc tests using Bonferroni correction revealed that there were increases in PT time from 0 hour to 4 hours (17.82 ± 0.61 seconds vs 18.30 ± 0.59 seconds, respectively), from 0 hour to 24 hours (17.82 ± 0.61 seconds vs 18.48 ± 0.59 seconds, respectively), from 2 hours to 4 hours (17.89 ± 0.58 seconds vs 18.30 ± 0.59 seconds), from 2 hours to 24 hours (17.89 ± 0.58 seconds vs 18.48 ± 0.58 seconds), which were all statistically significant (p = 0.002 and p < 0.000, p < 0.000, p < 0.000, respectively). However, the increase in PT time from 0 hour to 2 hours (17.82 ± 0.61 seconds vs 17.89 ± 0.59 seconds, respectively) and from 4 hours to 24 hours (18.30 ± 0.59 vs 18.48 ± 0.59 seconds, respectively) were not statistically significant (p = 1, p = 0.428). A repeated measure ANOVA determined that mean PTTK time differed statistically significantly between time points F (3, 291) = 119.22, p < 0.001. Post hoc tests using Bonferroni correction revealed that there were increase in PTTK time from 0 hour to 2 hours (37.86 ± 1.04 seconds vs 39.94 ± 1.07 seconds, respectively), from 0 hour to 4 hours (37.86 ± 1.04 seconds 80vs 42.34 ± 1.11 seconds, respectively), from 0 hours to 24 hours (37.86 ± 1.04 seconds vs 44.93 ± 1.20 seconds), from 2 hours to 4 hours (39.94 ± 1.07 seconds vs 42.34 ± 1.11 seconds), from 2 hours to 24 hours (39.94 ± 1.07 seconds vs 44.93 ± 1.20 seconds) and from 4 hours to 24 hours (42.43 ± 1.11 vs 44.93 ± 1.20 seconds), which were all statistically significant at p < 0.001). Therefore, we conclude that there are no statistically significant differences in the PT and APTT between 0 and 2 hours. A longer timing (after 2 hours) from phlebotomy collection of blood from respondents elicited a statistically significant increase in the PT and APTT result. There were no statistically significant differences in the PT and APTT result determined 4 hours and 24 hours after phlebotomy. Longer timing from collection of blood from respondents elicited a statistically significant increment/increase in the clotting time using PTTK. Our data demonstrate that PT and APTT results are stable for 2 hours remaining constant regardless of storage conditions.

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Influence of temperature and storage duration on measurement of activated partial thromboplastin time, D‐dimers, fibrinogen, prothrombin time and thrombin time, in citrate‐anticoagulated whole blood specimens

Abstract: We demonstrate that a storage time interval up to 24 h for D-dimers, Fbg, PT, and TT, and 8 h for APTT at either RT or 4 °C is acceptable.

Cited by 33 publications

References 11 publications

Effects of storage time and temperature on coagulation tests and factors in fresh plasma

Effects of storage time and temperature on coagulation tests and factors in fresh plasma

Effect of Centrifuge Temperature on Routine Coagulation Tests

The Effect of Time and Temperature Variables on Some Routine Coagulation Tests among Subjects of African Descent in Sokoto, North Western Nigeria

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