Influence of temperature and solvent concentration on the kinetics of the enzyme carbonic anhydrase in carbon capture technology

Gladis, Arne; Gundersen, Maria T.; Fosbøl, Philip Loldrup; Woodley, John M.; Solms, Nicolas von

doi:10.1016/j.cej.2016.10.056

Cited by 43 publications

(36 citation statements)

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“…As regards the effect of temperature and bicarbonate concentration (solvent loading) on enzyme kinetics, it is interesting to compare results of the present investigation with those reported in the literature. At 2 M K 2 CO 3 , the increase of the k E with the temperature is in agreement with results by Ye and Lu8 and by Gladis et al . The observed decrease of the k E with the solvent loading is in agreement with Hu et al .…”

Section: Resultssupporting

confidence: 92%

“…Two groups of tests were carried out: the first group was carried out at enzyme concentration set at 0.3 and 0.6 kg m −3 , the second group was carried out at enzyme concentration set less than 0.018 kg m −3 . The first group of tests at large NovoCA concentrations (0.3 and 0.6 kg m −3 ) was aimed to be representative for the development of CO 2 capture processes by means of reactive absorption . The second group of tests was aimed to the assessment of kinetic parameters for enzyme catalyzed CO 2 conversion according to the theory described in the Materials and methods section.…”

Section: Resultsmentioning

confidence: 99%

“…However, some issues limit the development of technologies based on amines or alkaline solvents. The use of amines as a reactive solvent suffers some disadvantages when applied to CO 2 capture: the relatively high operating temperature lowering CO 2 solubility; high energy demand for solvent regeneration, pollution issues related to the formation of heat‐stable salts, and equipment corrosion . Other alkaline solvents forming bicarbonates have been proposed as environmentally friendly and economically competitive alternative to amines.…”

Section: Introductionmentioning

confidence: 99%

“…However, the data processing of measurements from absorption tests is affected by the selection of physicochemical parameters and kinetics of parallel reactions (mainly CO 2 hydroxylation). Indeed, Gladis et al . and Ye and Lu highlighted this issue.…”

Section: Introductionmentioning

confidence: 99%

“…As a matter of fact, the development of effective enzyme immobilization techniques – i.e., that allow an increase of CA stability and reuse of the biocatalyst – seems currently the most reliable strategy to establish a CO 2 capture process by enzymatic reactive absorption . From an economic point of view, the industrial development of such a biocatalyst can take advantages by using technical grade CA instead of pure CA ,.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of technical grade carbonic anhydrase as biocatalyst for CO₂ capture in potassium carbonate solutions

et al. 2017

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Reactive absorption promoted by carbonic anhydrase (CA, E.C. 4.2.1.1.) catalysis has been proposed for CO2 capture from exhaust gas as an alternative to the reactive absorption in amine solutions. Potassium carbonate solutions allow feasible CO2 capture assisted by CA. This paper reports on the characterization of a CA form supplied by Novozymes as a catalyst for CO2 capture in K2CO3 solutions at operating conditions relevant for industrial processes (2–3 M K2CO3, 298–313 K, 0–40% carbonate to bicarbonate conversion). CO2 absorption tests were carried out in a batch‐stirred cell apparatus at constant gas volume and temperature by measuring gas pressure decay. The enhancement ratio between CO2 absorption rate with and without the enzyme at biocatalyst concentration above 0.3 kg m−3 ranged between 5 and 8 depending on the solvent composition and temperature. Active enzyme aggregates formed at protein concentration larger than 0.3 kg/m3 and their contribution to the absorption rate enhancement was remarkable. Assessment of enzyme kinetics in homogeneous solutions at enzyme concentration lower than 0.018 kg·m−3 showed that kcat/KM increased with both temperature and salt concentration. kcat/KM ranged between 9.0·103 and 7.0·104 m3·kg−1·s−1 in agreement with literature data on carbonic anhydrase kinetics. Reliability of the adopted method for kinetic characterization was confirmed. The activity of enzyme aggregates formed in the carbonate solvent was verified and CA aggregates can be used to select the proper CA based biocatalyst for CO2 capture application as an alternative to free and immobilized enzyme. © 2017 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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