Influence of temperature and size on menthol anaesthesia in Chinese grass shrimp Palaemonetes sinensis (Sollaud, 1911)

Abstract

Chinese grass shrimp, Palaemonetes sinensis (Sollaud, 1911), is an economically important freshwater shrimp in China and adjacent areas. It is advisable to use anaesthesia in this species for certain handling and shipping operations; however, there have been no investigations into the recommended dosages. Here, the influence of five menthol concentrations (varying from 100 to 500 mg/L) on three different size classes of P. sinensis were examined at 8, 12, 16, 20, 24 and 28°C.Induction and recovery times for each shrimp were recorded, and effects of temperature, size and menthol dose on induction and recovery times were observed.Results showed that menthol dose, water temperature and shrimp size significantly influence anaesthesia in P. sinensis. Induction time decreased linearly with increasing water temperature and concentration of menthol, and increased with body weight.However, recovery times lengthened with concentration and temperature, and became shorter with body weight. Average body weight of the shrimps generally decreased after anaesthesia. Mortality of shrimps was correlated with temperature, dose and size. These results suggest that menthol is an effective rapid anaesthetic for P. sinensis, but there may be some disadvantages, including slow recovery and possible mortality for small shrimps and at higher temperatures and dosages.

Anaesthetic Effects of Eugenol on Grass Shrimp (Palaemonetes sinensis) of Different Sizes at Different Concentrations and Temperatures

“…Although some research has shown that these environmental factors mght affect eugenol anaesthesia efficiency in fish, these considerations have not yet been explored in crustaceans. Moreover, our previous study demonstrated that menthol dose, water temperature and shrimp size significantly influence anaesthesia and survival rate in P. sinensis 15 .…”

Anesthetic potential of different essential oils for two shrimp species, Farfantepenaeus paulensis and Litopenaeus vannamei (Decapoda, Crustacea)

“…Smaller shrimp have higher rate of anesthetic absorption due to the greater gill surface area in relation to body size (BOWNIK, 2015). Studies on P. sinensis anesthetized with menthol demonstrated a direct relationship between body size and sensitivity to anesthesia (LI et al, 2018a). However, pink shrimp are likely to be more sensitive to EOLA anesthesia than L. vannamei because even the post-larvae forms take a longer time to reach anesthesia (PARODI et al, 2012).…”

“…Furthermore, because they are natural products, the application of EOs may be more environmentally friendly and cost-effective (REVERTER et al, 2014). Essential oils have been demonstrated to be safe and effective in studies on Litopenaeus vannamei (PARODI et al, 2012), Macrobrachium rosenbergii (SAYDMOHAMED & PAL, 2009), Palaemonetes sinensis (LI et al, 2018a) and Penaeus monodon (JIANG et al, 2020).…”

ANESTHETIC EFFICACY OF CLOVE OIL AND 2-PHENOXYETHANOL ON DOCTOR FISH, <i>Garra rufa</i> (HECKEL, 1843)

“…Anesthetic concentration and various biological or environmental factors significantly affect the anesthesia of fish (Santos et al, 2015;Li et al, 2018;Mitjana et al, 2018). Changes in water temperature have been shown to affect induction and recovery time in various fish species (Hamackova et al, 2004;Mylonas et al, 2005;Zahl et al, 2009;Santos et al, 2015;Skår et al, 2017).…”