Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix

Duodu, Samuel; Holst‐Jensen, Arne; Skjerdal, Taran; Cappelier, Jean‐Michel; Pilet, Marie-France; Lončarević, Semir

doi:10.1016/j.fm.2010.04.012

Cited by 48 publications

(37 citation statements)

References 43 publications

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“…The PCR efficiency (E) was calculated by using the equation E ϭ 10 Ϫ1/s . To quantify mRNA transcript levels, the C T (threshold cycle) values of the target gene and the reference gene (gerAA) from the same sample were transformed using the following term E ϪCT , and the expression levels of the target genes were normalized by dividing the transformed C T target by the transformed C T reference (53,54).…”

Section: Methodsmentioning

confidence: 99%

The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores

Borch-Pedersen

Lindbäck

Madslien

et al. 2016

Appl Environ Microbiol

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When nutrients are scarce, Bacillus species form metabolically dormant and extremely resistant spores that enable survival over long periods of time under conditions not permitting growth. The presence of specific nutrients triggers spore germination through interaction with germinant receptors located in the spore's inner membrane. Bacillus licheniformis is a biotechnologically important species, but it is also associated with food spoilage and food-borne disease. The B. licheniformis ATCC 14580/ DSM13 genome exhibits three gerA family operons (gerA, gerK, and ynd) encoding germinant receptors. We show that spores of IMPORTANCETo ensure safe food production and durable foods, there is an obvious need for more knowledge on spore-forming bacteria. It is the process of spore germination that ultimately leads to food spoilage and food poisoning. Bacillus licheniformis is a biotechnologically important species that is also associated with food spoilage and food-borne disease. Despite its importance, the mechanisms of spore germination are poorly characterized in this species. This study provides novel knowledge on germination of B. licheniformis spores. We characterize the germinant recognition profiles of the three germinant receptors present in B. licheniformis spores and demonstrate that the GerA germinant receptor cooperates with the Ynd and GerK germinant receptors to enable an effective germination response to L-amino acids. We also demonstrate that GerK is required for germination in response to the single germinant glucose. This study demonstrates the complex interactions between germinant receptors necessary for efficient germination of B. licheniformis spores. E ndospore formation is a phenotypic adaptation to unfavorable environmental conditions, which allows bacteria to persist in the environment in a dormant and extremely resistant state. Nevertheless, spores are able to continuously monitor the environment for conditions favorable for growth. Many members of the bacterial orders Bacilliales and Clostridiales are able to survive starvation by forming endospores, which are much more resistant to heat, chemicals, irradiation, and desiccation than the vegetative cells (1). However, upon exposure to nutrient germinants, spores can return to active growth within minutes in the process of germination (2-5).The nutrient-induced germination is initiated when specific nutrients are recognized by their cognate germinant receptors (GRs) located in the spores inner membrane (6-8). Multiple GR isoforms, with distinct nutrient specificities, have been characterized in different spore-forming species of the genera Bacillus and Clostridium (4, 9-15).The genetic organization and knowledge gained from functional studies suggest that the GRs are heterotrimeric complexes consisting of A, B, and C subunits, and at least in Bacillus, they are all required for the formation of a functional receptor (3, 16).Genes encoding GR homologs are often organized in polycistronic so-called gerA family operons, encoding the A, B...

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Section: Methodsmentioning

confidence: 99%

The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores

Borch-Pedersen

Lindbäck

Madslien

et al. 2016

Appl Environ Microbiol

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show abstract

“…Recently, efforts are being made to enrich current knowledge conducting studies in situ. This is commonly done by artificially inoculating food with high numbers of L. monocytogenes and following expression of selected genes of interest by RT--qPCR as well as performing experiments that determine virulence potential in vitro (for example adhesion, biofilm formation, invasion) and in vivo (for example, mouse infection) (Olesen et al, 2010;Duodu et al, 2010;Rieu et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…A protocol for the preparation of a sterile juice, starting from the two food matrices used in this study, minced meat and fermented sausage, was developed in order to avoid interference by the resident microbiota. This approach has been previously adopted in order to circumvent problems of sensitivity in the downstream processes (Duodu et al, 2010, Ligowaska et al, 2011.…”

Section: Resultsmentioning

confidence: 99%

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Strain dependent expression of stress response and virulence genes of Listeria monocytogenes in meat juices as determined by microarray

Rantsiou

Greppi

Garosi

et al. 2012

International Journal of Food Microbiology

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“…A técnica de RT-PCR é vantajosa por ser mais rápida e sensível quando comparada com métodos convencionais de quantificação de RNA, como o Northen blot, que permite o estudo do perfil de expressão de RNA mensageiro, por meio de eletroforese em gel, seguida de hibridação com sonda radioativa ou fluorescente (CAREY et al, 2008;DUODU et al 2010;LIGOWSKA et al, 2011;WERBROUCK et al, 2007).…”

Section: Análise Quantitativa Pela Reação Em Cadeia Da Polimerase Em unclassified

Avaliação da expressão de genes de virulência por Listeria monocytogenes em alimentos modelos

Silva¹

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Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix

Cited by 48 publications

References 43 publications

The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores

The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores

Strain dependent expression of stress response and virulence genes of Listeria monocytogenes in meat juices as determined by microarray

Avaliação da expressão de genes de virulência por Listeria monocytogenes em alimentos modelos

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