Influence of Sex on the Outcome of Autologous Chondrocyte Implantation in Chondral Defects of the Knee

Kreuz, Peter; Müller, Sebastian; Keudell, Arvind von; Tischer, Thomas; Kaps, Christian; Niemeyer, Philipp; Erggelet, Christoph

doi:10.1177/0363546513489262

Cited by 67 publications

(67 citation statements)

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“…Donor samples differed in age and gender but there were no obvious indications that this affected the potency of spheroids and confirmed observations in clinical practice where no significant differences in regeneration of condyle defects in male and female donors was observed [24]. The newly formed tissue mainly consisted of homogenous distributed cells filling up the defect, which was covered by a multi/monolayer of elongated cells arranged parallel to the surface with a smooth appearance attached to the surface of the native cartilage tissue.…”

Section: Discussionsupporting

confidence: 79%

An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant

Bartz¹,

Meixner²,

Giesemann³

et al. 2016

J Transl Med

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BackgroundCell-based therapies such as autologous chondrocyte implantation are promising therapeutic approaches to treat cartilage defects to prevent further cartilage degeneration. To assure consistent quality of cell-based therapeutics, it is important to be able to predict the biological activity of such products. This requires the development of a potency assay, which assesses a characteristic of the cell transplant before implantation that can predict its cartilage regeneration capacity after implantation. In this study, an ex vivo human cartilage repair model was developed as quality assessment tool for potency and applied to co.don’s chondrosphere product, a matrix-associated autologous chondrocyte implant (chondrocyte spheroids) that is in clinical use in Germany.MethodsChondrocyte spheroids were generated from 14 donors, and implanted into a subchondral cartilage defect that was manually generated in human articular cartilage tissue. Implanted spheroids and cartilage tissue were co-cultured ex vivo for 12 weeks to allow regeneration processes to form new tissue within the cartilage defect. Before implantation, spheroid characteristics like glycosaminoglycan production and gene and protein expression of chondrogenic markers were assessed for each donor sample and compared to determine donor-dependent variation.ResultsAfter the co-cultivation, histological analyses showed the formation of repair tissue within the cartilage defect, which varied in amount for the different donors. In the repair tissue, aggrecan protein was expressed and extra-cellular matrix cartilage fibers were present, both indicative for a cartilage hyaline-like character of the repair tissue. The amount of formed repair tissue was used as a read-out for regeneration capacity and was correlated with the spheroid characteristics determined before implantation. A positive correlation was found between high level of aggrecan protein expression in spheroids before implantation and a higher regeneration potential after implantation, reflected by more newly formed repair tissue.ConclusionThis demonstrated that aggrecan protein expression levels in spheroids before implantation can potentially be used as surrogate potency assay for the cartilage cell transplant to predict its regenerative capacity after implantation in human patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-016-1065-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 79%

An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant

Bartz¹,

Meixner²,

Giesemann³

et al. 2016

J Transl Med

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“…The present population consisted of 565 patients. In concordance with the present study, Kreuz et al [25] recently found a (somewhat smaller) gender difference in Lysholm score both before and after autogenous chondrocyte implantation in 52 patients. Briggs et al [8] did not find a gender difference in 488 healthy subjects without knee problems.…”

Section: Discussionsupporting

confidence: 93%

Symptoms and function in patients with articular cartilage lesions in 1,000 knee arthroscopies

Solheim

Krokeide

Melteig

et al. 2014

Knee Surg Sports Traumatol Arthrosc

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“…Autologous chondrocyte implantation (ACI) in the knee joint using a periosteal flap [1] has become a promising treatment with encouraging results for femoral and patellar cartilage defects [2][3][4][5][6]. In the last two decades since the first study by Brittberg et al, its further developments including the third generation autologous chondrocyte implantation using collagen I/III scaffolds has become increasingly popular for treatment of large full thickness cartilage defects.…”

Section: Introductionmentioning

confidence: 99%

Revision surgery after third generation autologous chondrocyte implantation in the knee

Niethammer

Valentin

Ficklscherer

et al. 2015

International Orthopaedics (SICOT)

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Influence of Sex on the Outcome of Autologous Chondrocyte Implantation in Chondral Defects of the Knee

Abstract: Autologous chondrocyte implantation is a promising treatment option for full-thickness cartilage defects of male and female knee joints. Female patients with patellar defects have worse prognostic factors.

Cited by 67 publications

References 46 publications

An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant

An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant

Symptoms and function in patients with articular cartilage lesions in 1,000 knee arthroscopies

Revision surgery after third generation autologous chondrocyte implantation in the knee

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