Influence of Scaffold Stiffness on Subchondral Bone and Subsequent Cartilage Regeneration in an Ovine Model of Osteochondral Defect Healing

Schlichting, Karin; Schell, Hanna; Kleemann, Ralf U.; Schill, Alexander; Weiler, Andreas; Duda, Georg N.; Epari, Devakara R.

doi:10.1177/0363546508322899

Cited by 86 publications

(66 citation statements)

References 44 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…10 For the evaluation of osteochondral reconstructions, histologic and mechanical examination protocols are suggested. 15 Mechanical properties of healthy cartilage and cartilage regenerates have been thoroughly investigated. 16,17 The use of equilibrium modulus by creep indentation testing has been used in several animal models, including the mini pig.…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Jagodzinski

Liu

Guenther

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

This study investigates the effects of seeding a chondrogenic and osteogenic scaffold with a bone marrowderived cell concentrate (BMCC) and reports the histological and mechanical properties 3 months after implantation in the miniature pig. Twenty defects (7 · 10 mm) were created in the femoral condyles of 10 miniature pigs. The defects were left empty (E), filled with the grafted cylinder upside down (U) or with a combined scaffold (S) containing a spongious bone cylinder (Tutobone Ò ) covered with a collagen membrane (Chondrogide Ò ). In a fourth group, the same scaffolds were implanted but seeded with a stem cell concentrate (S + BMCC). The animals were stained with calcein green after 2 weeks and xylenol orange after 4 weeks. After 3 months, the animals were sacrificed, and a mechanical analysis (Young's modulus), macroscopic, and histologic (ICRS Score) examination of the specimens was conducted. Young's modulus in the periphery was significantly lower for group E (67.5 -15.3 kPa) compared with untreated controls (171.7 -21.6 kPa, p < 0.04). Bone defects were smaller in group S (10% -8%) compared with E (27% -7%; p < 0.05). There was a trend toward smaller bony defects on comparing groups E and S + BMCC (11% -8%; p = 0.07). More red fluorescence was detected in group S + BMCC (2.3% -1.1%) compared with groups E (0.4% -0.2%) and U (0.5% -0.2%, p < 0.03). ICRS scores were higher for groups S (25.3 -3.8) and S + BMCC (26.2 -5.2, p < 0.01). In this animal model of osteochondral defects, stem cell concentrates enhance new bone apposition but fail to improve mechanical properties or histological appearance of cartilage regenerates in critical-sized defects.

show abstract

Section: Introductionmentioning

confidence: 99%

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Jagodzinski

Liu

Guenther

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

show abstract

“…This is particularly important in differentiation of cells, as well as the mechanotransductive signaling between cells that facilitate successful regeneration of anisotropic tissues. Cell-free graft techniques, which use biocompatible and degradable materials as a scaffold to support endogenous tissue regeneration, show promise in animal models but have yet to find clinical success [36][37][38] . Cell-seeded, biphasic scaffolds may serve as an integrated solution to reca pitulate the osteochondral interface and underlying bone [37,39] , but despite the success in pre-clinical stu dies, only three biphasic osteochondral scaffolds have extensive clinical application [40] .…”

Section: Drawbacks Of Current Tissue Engineering Approaches For Osteomentioning

confidence: 99%

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Datta

Dhawan

et al. 2024

IJB

View full text Add to dashboard Cite

Abstract:Osteochondral tissue regeneration has remained a critical challenge in orthopaedic surgery, especially due to complications of arthritic degeneration arising out of mechanical dislocations of joints. The common gold standard of autografting has several limitations in presenting tissue engineering strategies to solve the unmet clinical need. However, due to the complexity of joint anatomy, and tissue heterogeneity at the interface, the conventional tissue engineering strategies have certain limitations. The advent of bioprinting has now provided new opportunities for osteochondral tissue engineering. Bioprinting can uniquely mimic the heterogeneous cellular composition and anisotropic extra-cellular matrix (ECM) organization, while allowing for targeted gene delivery to achieve heterotypic differentiation. In this perspective, we discuss the current advances made towards bioprinting of composite osteochondral tissues and present an account of challenges-in terms of tissue integration, long-term survival, and mechanical strength at the time of implantation-required to be addressed for effective clinical translation of bioprinted tissues. Finally, we highlight some of the future trends related to osteochondral bioprinting with the hope of in-clinical translation. Keywords: bioprinting; osteochondral injuries; zonal anisotropy; bioink; tissue engineering

show abstract

“…osteoblasts require stiffer materials (150MPa), whereas embryonic cardiomyocytes require softer materials (modulus 1kPa) for regeneration of bone and cardiac tissue respectively. 72,73 As the materials aim to mimic the ECM that surrounds the cells, especially in the heart; the materials also need to be able to be structurally capable of withstanding the dynamic forces of the pulsatile heart.…”

Section: Physical Cuesmentioning

confidence: 99%

Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration

Wickham¹

2015

View full text Add to dashboard Cite

Linköping 2015Cover: Scanning Electron Microscopy image of a mesenchymal cell on polycaprolactone fibers.During the course of the research presented in this thesis, Abeni Wickham, was enrolled in Forum Scientium, a multidisciplinary graduate school at Linköping University. ColophonThis thesis is not only the summation of 5 years of research, but also a material manifestation of my happiness.It has not been straightforward and I have had numerous failures, disappointments and doubts. These have always been positive moments, as each difficult moment gave me the chance to choose a different path to still achieve my research goals.I have spent the past few years fighting for what I love the most; answering the questions that I ask myself every day. I have been lucky to have two supervisors, Daniel and Bo who pushed me to be better scientifically and family/friends who kept me grounded.During this work, I found who I really am; this thesis is a representation of me.ii Abstract Nature has had millions of years to perfect the structural components of the human body, but has also produced the dysfunctions that result in the cancers and diseases, which ruin that perfection. Congenital heart defects, and myocardial infarction lead to scarring that remodels heart muscle, decreasing the contractility of the heart, with profound consequences for the host. Regenerative medicine is the study of strategies to return diseased body parts to their evolutionarily optimum structure.Cells alone cannot develop into functional tissue, as they require mechanical support and chemical signals from the extracellular matrix in order to play the correct role in the body. In order to imitate the process of tissue formation optimized by nature, scaffolds are developed as the architectural support for tissue regeneration. To mimic the elasticity and strength seen in the heart muscle is one of the major scientific conundrums of our time. The development of new multifunctional materials for scaffolds is an accepted solution for repairing failing heart muscle. In this thesis I accept the notion that endogenous cardiac cells can play a major role in addressing this problem, if we can attract them to the site of defect or injury and make them proliferate. I then proceed to show how improving on a commonly used synthetic polymer was used to develop two new biomaterials.Polycaprolactone (PCL) fibers and sheets were studied for their ability to adsorb proteins based on their surface energies. We found that although the wettability of the PCL might be similar to positive controls for cell attachment, the large differences in surface energies may account for the increased serum protein adsorption and limit cell adhesion. The effect of fiber morphology was then investigated with respect to proliferation of mesenchymal stem cells and cardiac progenitor cells. PCL was also mechanically enhanced with thiophene conjugated single walled carbon nanotubes (T-CNT); where small concentrations of the T-CNT allowed for a 2.5 fold increase in the percentage of elong...

show abstract

Influence of Scaffold Stiffness on Subchondral Bone and Subsequent Cartilage Regeneration in an Ovine Model of Osteochondral Defect Healing

Cited by 86 publications

References 44 publications

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration

Contact Info

Product

Resources

About