Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells

Taqvi, Sabia; Roy, Krishnendu

doi:10.1016/j.biomaterials.2006.05.052

Cited by 61 publications

(38 citation statements)

References 28 publications

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“…In addition, an increase in polymer concentration resulted in an increased scaffold compression modulus with a concomitant significantly enhanced hematopoiesis. Finally, higher cell seeding densities as well as co-culture with marrow-derived stromal cells increased HPC generation [93]. Poly (L)-lactic coglycolic acid has been used as an in vitro-model for HSC and MSC homing and engraftment and tissue engineering purposes [94].…”

Section: Foam-like Systemsmentioning

confidence: 99%

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Gottwald¹

2017

ATROA

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Hematopoietic stem cell niches are perhaps the best described niche system in mammals. The niches themselves, as well as their cellular and structural constituents and factors that play a role in maintaining the niche structure and function, are being refined on a more or less daily basis. Despite this, it seems as if the more we know the harder it gets to mimic the in vivo-situation using in vitro-systems. This is due to the fact that hematopoiesis is a multi step maturation process leading to HSC heterogeneity. Subpopulations of HSCs and niche supporting cells can be defined depending on characteristics such as their potency of leading to successful reconstitution of sub lethally irradiated mice in serial transplantation experiments or, with less scientific impact, clonogenic assays. Since the bone marrow obviously provides all necessary information to maintain the stem cell pool constant and to adapt the number of blood cells according to physiologic needs, it has been the goal to engineer artificial niches that display at least one or several of the major characteristics of the in vivosituation to make use of these systems for not only fundamental research purposes but, moreover, also for clinical applications. This review will give an overview of approaches to engineering artificial hematopoietic niches with a focus on the complexity/dimensionality of the systems used.

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Section: Foam-like Systemsmentioning

confidence: 99%

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Gottwald¹

2017

ATROA

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“…As previously described, 33 undifferentiated mouse R1 ES cells were maintained and expanded on irradiation-inactivated STO cells in Dulbecco's modified Eagle's medium (Invitrogen) containing 20% defined fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, 50 mM 2-mercaptoethanol, 100 U=mL penicillin, and 100 mg=mL streptomycin. The LIF-producing STO cells can be replaced by mitomycin-inactivated murine embryonic fibroblasts with supplementation of LIF (Chemicon).…”

Section: Es Cell Culture and Differentiationmentioning

confidence: 99%

Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8⁺T Cells from Embryonic Stem Cells and Thymic Progenitors

Lin

Nie

Tucker

et al. 2010

Tissue Engineering Part A

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Generation of early T cells by coculturing stem cells on notch-ligand-expressing OP9 stromal cells (OP9-DL1) has been widely reported. However, further differentiation of these cells into mature, antigen-specific, functional T cells, without retroviral transduction of T cell receptors (TcRs), is yet to be achieved. In the thymic niche this differentiation is controlled by the interaction of developing TcRs with major histocompatibility (MHC) molecules on stromal cells. We hypothesized that by providing exogenous antigen-specific MHC=TcR signals, stem and progenitor cells could be engineered into functional, effector T cells specific for the same antigen. Here we demonstrate that both thymus-derived immature T cells (double positive [DP]: CD4 þ CD8 þ ) and mouse embryonic stem cells can be efficiently differentiated into antigen-specific CD8 þ T cells using either MHC tetramers or peptide-loaded stromal cells. DP cells, following MHC=TcR signaling, retained elevated recombination activating gene-1 levels, suggesting continuing TcR gene rearrangement. Both DP and embryonic stem-cell-derived CD8 þ T cells showed significant cytotoxic T lymphocytes activity against antigen-loaded target cells, indicating that these cells are functional. Such directed differentiation strategy could provide an efficient method for generating functional, antigen-specific T cells from stem cells for potential use in adoptive T cell therapy.

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“…Polymer materials in the form of elastomeric gels, porous bodies, films, and particles are suitable substrates for a biomatrix, and can be applied for the culture of complex living systems. In particular, the porous scaffold fabricated on the cells culture substrate promotes cell growth and survival and reproduces the three-dimensional environmental microcosm that is normally present in the living body [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, polymers are flexible and cheap when compared to glass materials. Polymer materials are often used as cell culture sheets [3], and polymer substrates with fabricated microstructures have been reported to support the culture of biological cells [3,4]. Polymer materials in the form of elastomeric gels, porous bodies, films, and particles are suitable substrates for a biomatrix, and can be applied for the culture of complex living systems.…”

Section: Introductionmentioning

confidence: 99%

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A Transparent Polyimide Film as a Biological Cell Culture Sheet with Microstructures

Maenosono¹,

Saito²,

Nishioka³

2014

JBNB

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The research on stem cell cultures has attracted much attention due to the recent development of regenerative medicine. Therefore, higher functionalities for devices used for culturing cells are strongly demanded. In this study, we fabricated cell culture sheets using transparent polyimide (PI), parylene (PA), and polyetheretherketone (PEEK) to make polymer materials that had microstructures. We then cultured stromal marrow cells (OP9) on them and investigated the cell alignment within the microstructures. Hot embossing was used to fabricate the microstructures with a width and depth of 5 μm on the polymer substrates. Cultivation of the cells was confirmed on the transparent PI and PA sheets, however, it was not observed on the PEEK sheet. Slight alignment of the cells was also observed along with the microstructures.

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Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells

Cited by 61 publications

References 28 publications

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8⁺T Cells from Embryonic Stem Cells and Thymic Progenitors

A Transparent Polyimide Film as a Biological Cell Culture Sheet with Microstructures

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Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells

Cited by 61 publications

References 28 publications

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8+T Cells from Embryonic Stem Cells and Thymic Progenitors

A Transparent Polyimide Film as a Biological Cell Culture Sheet with Microstructures

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Product

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Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8⁺T Cells from Embryonic Stem Cells and Thymic Progenitors