Influence of rutin treatment on biochemical alterations in experimental diabetes

Fernandes, Ana Angélica Henrique; Novelli, Ethel L. B.; Okoshi, Katashi; Okoshi, Marina Politi; Muzio, Bruno Di; Guimarães, Julliano F. Campos; Fernandes, Adalton Mazetti

doi:10.1016/j.biopha.2009.08.007

Cited by 125 publications

(65 citation statements)

References 50 publications

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“…Then, these events associated with the potential decrease of ADA activity in serum and therefore increased vascular of adenosine could suggest that Rutin plays an important role in prevention of atherosclerosis in diabetes, especially when considering the antioxidant activity of the flavonoid. Also, this property can to contribute to reduce the hepatic enzymes activity observed in the diabetic rats (Fernandes et al, 2010). These results are in accordance with other studies that have showed the hypoglycemic effects (Gandhi et al, 2011), and our findings showed the significant reduction of glucose the 20.5% end of the treatment with Rutin.…”

Section: Discussionsupporting

confidence: 93%

Rutin restores adenosine deaminase activity in serum and the liver and improves biochemical parameters in streptozotocin-induced diabetic rats

Chielle

Bonfanti

Bona

et al. 2016

Rev. bras. plantas med.

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denosine deaminase (ADA) is a critical control point in the regulation of adenosine levels. This study aimed to investigate the effects of a polyphenolic flavonoid, rutin, on the activity of ADA in serum, the cerebral cortex, liver, kidney, and biochemical parameters in diabetic rats. The animals were divided into four groups (n=6) for the following treatments: control; diabetic (streptozotocin 55 mg/kg); diabetic with rutin (100 mg/kg/day); diabetic with glibenclamide (10 mg/kg/day). After 30 days, ADA activity and biochemical parameters were analyzed. The ADA activity in the serum was significantly elevated in the diabetic group compared to the control group (p<0.01). The treatment with rutin prevented the increase in ADA activity in the STZinduced rats when compared to control group. Our data showed that rutin reduced glucose, LDL levels, and hepatic enzymes in comparison with the control group. These results demonstrate that the increase of ADA activity observed in diabetic rats may be an important indicator of the immunopathogenesis of hyperglycemic disorders and suggest that rutin is important for regulating the enzymatic activities associated with immune, hyperglycemic, and inflammatory response in diabetes mellitus.Keywords: rutin; adenosine deaminase activity; streptozotocin; Wistar rats; diabetes.RESUMO: Rutina restaura a atividade da Adenosina desaminase no soro e no fígado e melhora os parâmetros bioquímicos em ratos diabéticos induzidos por estreptozotocina. A Adenosina desaminase (ADA) representa um ponto de controle crítico na regulação dos níveis de adenosina. A rutina, um flavonóide polifenólico presente em muitas plantas, foi testado para verificar a sua influência na atividade da ADA no soro, córtex cerebral, fígado rim e parâmetros bioquímicos em ratos diabéticos. Os animais foram divididos em quatro grupos cada grupo com 6 animais), tal como: controle; diabética (estreptozotocina 55 mg/kg); diabética + rutina (100 mg/kg/dia); diabético + glibenclamida (10 mg/kg/dia). Após 30 dias foram analisadas a atividade da ADA sérica e tecidual e parâmetros bioquímicos. A atividade de ADA no soro foi significativamente elevada no grupo diabético quando comparado ao grupo controle (p<0,01). O tratamento com Rutina preveniu o aumento na atividade da ADA nos ratos diabéticos, quando comparado com o grupo controle. Os resultados mostraram que a rutina reduziu a glicose, os níveis de LDL e as enzimas hepáticas, em comparação com o grupo controle. Estes resultados mostram que o aumento da atividade da ADA observado em ratos diabéticos pode ser um indicador importante da imuno-patogênese de perturbações hiperglicêmicas e sugerem que a Rutina é importante na regulação das atividades enzimáticas associadas com a resposta imunitária, hiperglicêmica e inflamatória no Diabetes mellitus.

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Section: Discussionsupporting

confidence: 93%

Rutin restores adenosine deaminase activity in serum and the liver and improves biochemical parameters in streptozotocin-induced diabetic rats

Chielle

Bonfanti

Bona

et al. 2016

Rev. bras. plantas med.

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“…Compared with control animals, plasma ALT and AST levels were increased by factors of 3 and 2 respectively in the diabetic animals (data not shown). These results are consistent with the STZ model described by others (Barneo et al 1990, Hwang et al 2005, Fernandes et al 2009. Table 1 shows bw and blood glucose levels in control rats, diabetic rats (SID), diabetic rats treated with insulin (SIDCI), and these same three groups treated with DES or TEM.…”

Section: Assessment Of the Induced Diabetic State: Effect Of Insulin supporting

confidence: 90%

Hyperglycemia induces apoptosis in rat liver through the increase of hydroxyl radical: new insights into the insulin effect

Francés

Ronco²,

Monti³

et al. 2010

Journal of Endocrinology

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In this study, we analyzed the contribution of hydroxyl radical in the liver apoptosis mediated by hyperglycemia through the Bax-caspase pathway and the effects of insulin protection against the apoptosis induced by hyperglycemia. Male adult Wistar rats were randomized in three groups: control (C) (sodium citrate buffer, i.p.), streptozotocin (STZ)-induced diabetic (SID) (STZ 60 mg/kg body weight, i.p.), and insulintreated SID (SIDCI; 15 days post STZ injection, SID received insulin s.c., twice a day, 15 days). Rats were autopsied on day 30. In liver tissue, diabetes promoted a significant increase in hydroxyl radical production which correlated with lipid peroxidation (LPO) levels. Besides, hyperglycemia significantly increased mitochondrial BAX protein expression, cytosolic cytochrome c levels, and caspase-3 activity leading to an increase in apoptotic index. Interestingly, the treatment of diabetic rats with desferoxamine or tempol (antioxidants/ hydroxyl radical scavengers) significantly attenuated the increase in both hydroxyl radical production and in LPO produced by hyperglycemia, preventing apoptosis by reduction of mitochondrial BAX and cytosolic cytochrome c levels. Insulin treatment showed similar results. The finding that co-administration of antioxidants/hydroxyl radical scavengers together with insulin did not provide any additional benefit compared with those obtained using either inhibitors or insulin alone shows that it is likely that insulin prevents oxidative stress by reducing the effects of hydroxyl radicals. Importantly, insulin significantly increased apoptosis inhibitor protein expression by induction of its mRNA. Taken together, our studies support that, at least in part, the hydroxyl radical acts as a reactive intermediate, which leads to liver apoptosis in a model of STZ-mediated hyperglycemia. A new anti-apoptosis signal for insulin is shown, given by an increase of apoptosis inhibitor protein.

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“…1b. Currently, several studies have reported that Rutin [58,59], Quercetin [60,61], and Catechin [62,63] are a group of flavonoids by isolated from T. sinensis plant, that have been shown to present blood glucose lowering properties in animal model and being potent compounds in the inhibition of alpha-amylase.…”

Section: Discussionmentioning

confidence: 99%

Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>

Riyaphan¹,

Jhong²,

Tsai³

et al. 2017

Preprint

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Influence of rutin treatment on biochemical alterations in experimental diabetes

Cited by 125 publications

References 50 publications

Rutin restores adenosine deaminase activity in serum and the liver and improves biochemical parameters in streptozotocin-induced diabetic rats

Rutin restores adenosine deaminase activity in serum and the liver and improves biochemical parameters in streptozotocin-induced diabetic rats

Hyperglycemia induces apoptosis in rat liver through the increase of hydroxyl radical: new insights into the insulin effect

Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>

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