Influence of Red Blood Cells on Lung Function in an ex Vivo Rat Heart-Lung Model

Fukuse, Tatsuo; Albes, Johannes M.; Takahashi, Yutaka; Brandes, Harald; Hausen, Bernard; Schäfers, Hans‐Joachim

doi:10.1006/jsre.1995.1182

Cited by 33 publications

(44 citation statements)

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“…An isolated lung model previously described in detail was employed [20]. The isolated lungs were flush perfused via the pulmonary artery with 20 mL of the respective K + -modified ECS, and inflated with 10 mL air before hypothermic storage for 2 h at 48C in the respective preservation solution.…”

Section: Methodsmentioning

confidence: 99%

Preservation of intraalveolar surfactant in a rat lung ischaemia/reperfusion injury model

et al. 2000

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Ischaemia/reperfusion (I/R) injury, a major problem in clinical lung transplantation, is associated with surfactant dysfunction. The present study aimed to test the hypothesis that preservation related improvements in post-ischaemic lung function are associated with improved ultrastructural preservation of pulmonary surfactant.Rat lungs were flush perfused with modified Euro-Collins solutions (ECS), stored for 2 h at 48C, and reperfused for 40 min. Lungs were preserved with conventional (ECS 115: 115 mmol . L -1 K + ), medium-K + (ECS 40: 40 mmol . L -1 K + ), or low-K + (ECS 10: 10 mmol . L -1 K + ) ECS. Functional parameters were monitored during reperfusion (n=10 per group). After reperfusion, left lungs were prepared for electron microscopical and stereological analysis of surfactant (n=5 per group).In all three experimental groups notable I/R injury developed which was lowest in ECS 40 as indicated by significantly less intraalveolar oedema, higher perfusate oxygenation, and lower peak inspiratory pressure. This was associated with a significantly superior preservation of the ultrastructure of the surface active surfactant subtype tubular myelin in ECS 40 compared with ECS 115 and ECS 10. Stereological analysis revealed that the relative amount of tubular myelin was highest in ECS 40 (mean SEM; 6.2 0.8%) compared with ECS 115 (3.0 1.0%) and ECS 10 (2.7 1.6%).Analysis of surfactant in its natural location within the organ showed that the severity of ischaemia/reperfusion injury correlates with differences in intraalveolar surfactant composition. Improved post-ischaemic respiratory function achieved by medium-K + Euro-Collins solution is associated with superior ultrastructural preservation of tubular myelin. It is concluded that the integrity of surface active tubular myelin represents an important criterion for the assessment of lung preservation quality. Eur Respir J 2000; 15: 526±531.

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Section: Methodsmentioning

confidence: 99%

Preservation of intraalveolar surfactant in a rat lung ischaemia/reperfusion injury model

et al. 2000

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“…Post-experimental visualisation of histological lung sections was used to confirm the even distribution of exogenous surfactant in the lung. Extracorporeal reperfusion was performed using the extracorporeal heart-lung circuit described in detail by FUKUSE et al [31]. In short, the circuit consisted of a reservoir, a temperature probe (for perfusate temperature monitoring), a roller pump to raise the perfusate to the oxygenators, a blood filter and two membrane oxygenators.…”

Section: Experimental Settingmentioning

confidence: 99%

Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

Mühlfeld¹,

Schaefer²,

Becker³

et al. 2008

European Respiratory Journal

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The optimal timing of exogenous surfactant application to reduce pulmonary injury and dysfunction was investigated in a rat lung ischaemia and reperfusion injury model.Lungs were subjected to flush perfusion, surfactant instillation, cold ischaemia (4uC, 4 h) and reperfusion (60 min). Animals received surfactant before (group 1) or at the end (2) of ischaemia, or during reperfusion (3) or not at all (4). Control groups included ''worst case'' without Perfadex and surfactant (5), ''no injury'' without (6) or with surfactant (7), and ischaemia with pre-ischaemic surfactant (8). Intra-alveolar oedema and blood-air barrier injury were estimated by light and electron microscopic stereology. Perfusate oxygenation and pulmonary arterial pressure (Ppa) were determined during reperfusion in groups 1 to 4.Intra-alveolar oedema was almost absent in groups 1, 6, 7 and 8, pronounced in 2, 3 and 4, and severe in 5. Blood-air barrier injury was moderate in groups 1 and 8, slightly pronounced in 2, 3 and 4, extensive in 5 and almost absent in 6 and 7. Perfusate oxygenation was significantly higher in group 1 compared with groups 2 to 4. Ppa did not differ between the groups.In conclusion, exogenous surfactant attenuates intra-alveolar oedema formation and blood-air barrier damage and improves perfusate oxygenation in the rat lung, especially when applied before ischaemic storage.

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“…The lungs were allowed to deflate during the preparation. Thereafter, the lungs were reinflated with 10 ml of room air, the heart-lung block was then immersed in the respective cold solution and stored at 4°C for 4 h. An extracorporeal circuit, described by Fukuse et al [17], was modified and used. The perfusion circuit was primed with 600 ml of Krebs-Henseleit solution containing washed bovine red blood cells.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Euro-Collins Solution, Low-Potassium Dextran Solution Containing Glucose, and ET-Kyoto Solution for Lung Preservation in an Extracorporeal Rat Lung Perfusion Model

et al. 1998

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We have recently developed a modified ET-Kyoto solution by adding N-acetylcysteine, nitroglycerin, and dibutyryl cyclic adenosine monophosphate to the previously reported ET-Kyoto solution. The purpose of this study was to compare the new ET-Kyoto solution with the regular Euro-Collins solution and a low-potassium dextran solution containing glucose (Perfadex^®) in an isolated rat lung perfusion model. Rat lung blocks were preserved with ET-Kyoto solution, with Euro-Collins solution, or with Perfadex for 4 h. Arterial oxygen tension, shunt fraction, peak inspiratory pressure, mean pulmonary arterial pressure, and pulmonary vascular resistance were assessed up to 50 min after reperfusion. ET-Kyoto solution provided a significantly better lung function after reperfusion than Euro-Collins solution and Perfadex, while Perfadex was also superior to the Euro-Collins solution. We conclude that lung preservation with ET-Kyoto solution is significantly superior to Euro-Collins solution and to Perfadex in an isolated rat lung perfusion model.

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Influence of Red Blood Cells on Lung Function in an ex Vivo Rat Heart-Lung Model

Cited by 33 publications

References 0 publications

Preservation of intraalveolar surfactant in a rat lung ischaemia/reperfusion injury model

Preservation of intraalveolar surfactant in a rat lung ischaemia/reperfusion injury model

Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

Comparison of Euro-Collins Solution, Low-Potassium Dextran Solution Containing Glucose, and ET-Kyoto Solution for Lung Preservation in an Extracorporeal Rat Lung Perfusion Model

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