2005
DOI: 10.1016/j.femsle.2005.09.027
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Abstract: A pyocyanin overproducer with insertional inactivation of ptsP gene was isolated from a mini-Mu insertion library in Pseudomonas aeruginosa PA68. The mutation was complemented by a functional ptsP gene in trans. The pyocyanin-overproducing phenotype was also found in a ptsP mutant constructed by gene replacement in the P. aeruginosa PAO1 strain. Reporter plasmids with P(qscR)-lacZ, P(lasI)-lacZ and P(rhlI)-lacZ were constructed and the beta-galactosidase activity in the ptsP mutant/wild-type background was mea… Show more

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Cited by 32 publications
(29 citation statements)
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“…PCA and PYO were considered to have similar physiological and biochemical functions in cells (13); however, the different functional roles of PCA and PYO have been described in a few publications recently (10,16,28,57). Based on the methods used in this study, it would be quite difficult to compare our PCA and PYO measurements with those in other studies, where PCA was mainly assayed at pH 4.0 by UV spectroscopy of high-performance liquid chromatography at 248 nm, while PYO was assayed at pH 7.0 by measuring OD 520 (9,14,45,58,59). We first quantified the relative amounts of PCA and PYO produced by strains M18 and PAO1 and, interestingly, found that the main phenazine accumulated in strain M18 was PCA, especially at 28°C, while it was PYO in strain PAO1, especially at 37°C.…”
Section: Discussionmentioning
confidence: 91%
See 1 more Smart Citation
“…PCA and PYO were considered to have similar physiological and biochemical functions in cells (13); however, the different functional roles of PCA and PYO have been described in a few publications recently (10,16,28,57). Based on the methods used in this study, it would be quite difficult to compare our PCA and PYO measurements with those in other studies, where PCA was mainly assayed at pH 4.0 by UV spectroscopy of high-performance liquid chromatography at 248 nm, while PYO was assayed at pH 7.0 by measuring OD 520 (9,14,45,58,59). We first quantified the relative amounts of PCA and PYO produced by strains M18 and PAO1 and, interestingly, found that the main phenazine accumulated in strain M18 was PCA, especially at 28°C, while it was PYO in strain PAO1, especially at 37°C.…”
Section: Discussionmentioning
confidence: 91%
“…PCA was extracted at pH 4 and quantified in chloroform as described by Ge et al (14). Quantification of PYO production was done at neutral pH in chloroform as described by Essar et al (12,45,58).…”
mentioning
confidence: 99%
“…This report presents the first evidence that the phzA1 operon is activated by subinhibitory concentrations of Tet. The identification of several new genes involved in phenazine regulation was somewhat surprising, considering that a number of previous studies focused on this pathway (19,52). Since the mutants were initially selected when phzA1 expression was activated by subinhibitory Tet concentrations, these mutants might be missed by studies under other conditions.…”
Section: Discussionmentioning
confidence: 99%
“…To find out which steps of the PYO pathway were subject to catabolite repression, we grew the wild type and the crc mutant in liquid PPM amended with succinate and determined PCA and PYO levels in culture supernatants during the transition from exponential growth to stationary phase (ca. 5 ϫ 10 9 cells/ml) by methods previously described (6,31). PCA levels were elevated about 1.5-fold in the crc mutant PAO6673 from levels in the wild-type PAO1 and the complemented crc mutant (Table 2).…”
mentioning
confidence: 95%
“…d PCA was extracted from culture supernatants at pH 4 as described previously (6). PYO was extracted at neutral pH with chloroform and then reextracted with 0.1 M HCl as described previously (31). 2).…”
mentioning
confidence: 99%