Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors

Kraigher, Barbara; Kosjek, Tina; Heath, Ester; Kompare, Boris; Mandić-Mulec, Ines

doi:10.1016/j.watres.2008.08.006

Cited by 137 publications

(88 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among phylum Proteobacteria, β-Proteobacteria (16%) were dominant classes in the DWW, while α-(5.6%) and β-(25.3%) Proteobacteria were profound classes in the HWW. β-Proteobacteria was the most abundant class in the activated sludge bioreactor treating pharmaceutical residues [32], which corresponds to the microbial community in the HWW. As a result, the HWW may contain high antibiotic levels affecting the richness of β-Proteobacteria.…”

Section: Microbial Diversity and Comparison In The Dww And The Hwwmentioning

confidence: 99%

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

Ahn

Choi

2016

Water

View full text Add to dashboard Cite

Abstract:The community of whole microbes and antibiotic resistance bacteria (ARB) in hospital wastewater treatment plants (WWTP) receiving domestic wastewater (DWW) and hospital wastewater (HWW) was investigated. Samples from an influent of a secondary clarifier, at each treatment train, were characterized for the whole microbial community and ARB on the antibiotic resistance database, based on high-throughput pyrosequencing. The pyrosequencing analysis revealed that the abundance of Bacteroidetes in the DWW sample was higher (~1.6 times) than in the HWW sample, whereas the abundance of Proteobacteria in the HWW sample was greater than in the DWW sample. At the top twenty of the genus level, distinct genera were observed-Saprospiraceae in the DWW and Zoogloea in the HWW. Apart from the top twenty genera, minor genera showed various antibiotic resistance types based on the antibiotic resistance gene database.

show abstract

Section: Microbial Diversity and Comparison In The Dww And The Hwwmentioning

confidence: 99%

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

Ahn

Choi

2016

Water

View full text Add to dashboard Cite

show abstract

“…However, it is questionable if they adapt to pharmaceutical compounds present in wastewater only in very small quantities. Kraigher et al (2008) showed that a significant structural shift in the bacterial community caused by permanent PhAC presence occurred only at concentrations >50 lg L À1 , which are unlikely to occur in municipal WWTPs receiving domestic wastewaters.…”

Section: Heterotrophic Phac Biodegradation and Sludge Retention Timementioning

confidence: 99%

“…The SRT is a design criterion for WWTPs and strongly related to microbial growth. Nonetheless, the relation between SRT, microbial community structure and xenobiotic degradation performance is not fully understood and controversial findings have been reported (Clara et al, 2005;Gaulke et al, 2009;Kraigher et al, 2008;Saikaly et al, 2005;Schaar et al, 2010;Stasinakis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Active heterotrophic biomass and sludge retention time (SRT) as determining factors for biodegradation kinetics of pharmaceuticals in activated sludge

Majewsky

Gallé

Yargeau

et al. 2011

Bioresource Technology

View full text Add to dashboard Cite

a b s t r a c tThe present study investigates the biodegradation of pharmaceutically active compounds (PhACs) by active biomass in activated sludge. Active heterotrophs (X bh ) which are known to govern COD removal are suggested as a determining factor for biological PhAC removal as well. Biodegradation kinetics of five polar PhACs were determined in activated sludge of two wastewater treatment plants which differed in size, layout and sludge retention time (SRT).Results showed that active fractions of the total suspended solids (TSS) differed significantly between the two sludges, indicating that TSS does not reveal information about heterotrophic activity. Furthermore, PhAC removal was significantly faster in the presence of high numbers of heterotrophs and a low SRT. Pseudo first-order kinetics were modified to include X bh and used to describe decreasing PhAC elimination with increasing SRT.

show abstract

“…In this more functionally based T-RFLP approach instead of 16S rDNA genes, the functional gene of interest is amplified and used as the T-RFLP target. T-RFLP analysis has been used to examine many microbial communities (Ayala-del-Rio et al, 2004;Córdova-Kreylos et al, 2006;Friedrich et al, 2001;Grant et al, 2007;Hullar et al, 2006;Junier et al, 2008;Kent et al, 2003;Kraigher et al 2008;Li et al, 2007;Liu et al, 1997;Nagashima et al, 2003;Scully et al, 2005;Smith et al, 2005).…”

Section: Culture-independent Techniquesmentioning

confidence: 99%

Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

Chauhan¹,

Shagol²,

Yim³

et al. 2011

Korean Journal of Soil Science and Fertilizer

View full text Add to dashboard Cite

Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time-and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

show abstract

Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors

Cited by 137 publications

References 37 publications

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing

Active heterotrophic biomass and sludge retention time (SRT) as determining factors for biodegradation kinetics of pharmaceuticals in activated sludge

Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

Contact Info

Product

Resources

About