Influence of perfusion and cyclic compression on proliferation and differentiation of bone marrow stromal cells in 3-dimensional culture

Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures

“…To some extent, this was supported by tissue engineering experimental phenomena. For example, an experimental study showed that a combination of fluid perfusion (inlet flow rate: 10mL/min) and mechanical compression (compressive strain: 10%) could stimulate the bone cells resulting in larger amount of osteocalcin and higher Runx2 expression than those under fluid perfusion after 2-3 weeks culture (Jagodzinski et al 2008). …”

“…However, direct measurement of fluid-induced shear stress within scaffolds is not feasible, which prevents researchers from correlating the levels of fluid shear 5 stress to biochemical responses, see in particular (Gomes et al 2003;Jagodzinski et al 2008;Liu et al 2012a) and Table 1. Therefore, researchers are required to employ analytical predictions, based on idealised flow through a cylinder or two plates (Blecha et al 2010;Goldstein et al 2001), or estimate wall shear stress (WSS) magnitudes from existing computational models (Bancroft et al 2002;Grayson et al 2008;Vance et al 2005;Yu et al 2004), see Table 1.…”

“…when compared to applied fluid perfusion only (Jagodzinski et al 2008;Liu et al 2012a;Stops et al 2010). Interestingly, recent computational studies showed 7 that a combination of fluid perfusion and mechanical compression led to more ubiquitous stimulation of bone cells within a TE scaffold (Hendrikson et al 2014;Zhao et al 2015), in particular to both cells that had an attached and bridged configuration.…”

Toll-like receptor 2/6-dependent stimulation of mesenchymal stem cells promotes angiogenesis by paracrine factors

“…The same solution was used to irrigate the aspiration syringe before its use. The bioreactor was rotated at 5° per minute for 24 h. Then, the scaffolds were perfused with 500 mL DMEM Ham´s F-12 medium (Biochrom) containing 10 % FCS, 100 U/ mL penicillin and 200 µg/mL streptomycin (Biochrom), 0.5 µg/mL amphotericin B (Biochrom), 5 µg/mL ascorbic acid (Sigma-Aldrich), 0.02 nM/mL dexamethasone (Merck, Darmstadt, Germany) (Jagodzinski et al, 2004). In addition, the medium was supplemented with 30 ng/mL MALP-2.…”

Regulation of gene expression in articular cells is influenced by biomechanical loading

“…Among other things, cell proliferation can be improved significantly by biomechanical loading [12][13][14][15][16]. To what extent cell functions of different articular cells are affected by a defined biomechanical stimulus remains largely unknown.…”