Influence of oxygen in atmospheric-pressure argon plasma jet on sterilization of Bacillus atrophaeous spores

Lim, Jin-Pyo; Uhm, Han S.; Li, Shou-Zhe

doi:10.1063/1.2773705

Cited by 94 publications

(55 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By adding O 2 or increasing humidity, an increased concentration of ROS (e.g. O, O 2 - , OH, H 2 O 2 , 1 O 2 , O 3 ) was expected [43,44]. Especially the electrons of Ar plasma have enough energy to dissociate O-H bonds of water, so the gas humidity may increase the concentration of OH radicals [45].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Different Carrier Gases for Barrier Discharge Plasma Generation Compared to Chlorhexidine on the Survival of Pseudomonas aeruginosa Embedded in Biofilm in vitro

Matthes

Hübner

Bender

et al. 2014

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Because of its antimicrobial properties, nonthermal plasma could serve as an alternative to chemical antisepsis in wound treatment. Therefore, this study investigated the inactivation of biofilm-embedded Pseudomonas aeruginosa SG81 by a surface barrier-discharged (SBD) plasma for 30, 60, 150 and 300 s. In order to optimize the efficacy of the plasma, different carrier gases (argon, argon admixed with 1% oxygen, and argon with increased humidity up to approx. 80%) were tested and compared against 0.1% chlorhexidine digluconate (CHG) exposure for 600 s. The antimicrobial efficacy was determined by calculating the difference between the numbers of colony-forming units (CFU) of treated and untreated biofilms. Living bacteria were distinguished from dead by fluorescent staining and confocal laser scanning microscopy. Both SBD plasmas and CHG showed significant antimicrobial effects compared to the untreated control. However, plasma treatment led to a higher antimicrobial reduction (argon plasma 4.9 log₁₀ CFU/cm², argon with admixed oxygen 3 log₁₀ CFU/cm², and with increased gas humidity 2.7 log₁₀ CFU/cm² after 300 s) compared to CHG. In conclusion, SBD plasma is suitable as an alternative to CHG for inactivation of Pseudomonas aeruginosa embedded in biofilm. Further development of SBD plasma sources and research on the role of carrier gases and humidity may allow their clinical application for wound management in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficacy of Different Carrier Gases for Barrier Discharge Plasma Generation Compared to Chlorhexidine on the Survival of Pseudomonas aeruginosa Embedded in Biofilm in vitro

Matthes

Hübner

Bender

et al. 2014

Skin Pharmacol Physiol

View full text Add to dashboard Cite

show abstract

“…For 60 s treatment time the void size reaches its maximum with 10 cm and about 1.3 × 10 5 CFU could be inactivated. Lim et al (2007) used an APPJ with a coaxial electrode configuration comparable to figure 9d. The inner electrode has a stepped structure partially covered by a dielectric tube on the top of the electrode.…”

Section: Atmospheric Pressure Plasma Jetmentioning

confidence: 99%

Low temperature atmospheric pressure plasma sources for microbial decontamination

Ehlbeck¹,

Schnabel²,

Polák³

et al. 2010

J. Phys. D: Appl. Phys.

635

415

View full text Add to dashboard Cite

To cite this version:J EhlbeckAbstract. The aim of this article is to provide a survey of plasma sources at atmospheric pressure used for microbicidal treatment. In order to consider the interdisciplinary character of this topic an introduction and definition of basic terms and procedures is given for plasma as well as for microbicidal issues. The list of plasma sources makes no claim to be complete, but to represent the main principles of plasma generation at atmospheric pressure and to give an example of their microbicidal efficiency. The interpretation of the microbicidal results remain difficult due to the non standardized methods uses by different authors and due to the fact that small variations in the set up can change the results dramatically.

show abstract

“…There are several works on spores deactivation by atmospheric pressure plasma. For example, Iseki et al [13] used plasma of argon gas, Lim et al [20] used Ar/O 2 plasma discharge and Uhm et al [27] utilized argon gas plasma for spore deactivation. Recently, Connolly et al [5] used Helium/air plasma at atmospheric pressure for antimicrobial efficacy against E.coli in a plastic package.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Aspergillus flavus spores in a sealed package by cold plasma streamers

et al. 2016

View full text Add to dashboard Cite

The main objective of this study is to investigate the inactivation efficacy of cold streamers in a sealed package on pathogenic fungi Aspergillus flavus (A. flavus) spores that artificially contaminated pistachio surface. To produce penetrating cold streamers, electric power supply was adapted to deposit adequate power into the package. The plasma streamers were generated by an alternating high voltage with carrier frequency of 12.5 kHz which was suppressed by a modulated pulsed signal at frequency of 110 Hz. The plasma exposition time was varied from 8 to 18 min to show the effect of the plasma treatment on fungal clearance while the electrode and sample remained at room temperature. This proved a positive effect of the cold streamers treatment on fungal clearance. Benefits of deactivation of fungal spores by streamers inside the package include no heating, short treatment time and adaptability to existing processes. Given its ability to ensure the safety and longevity of food products, this technology has great potential for utilization in food packaging and processing industry. In this study, moisture and pH changes of pistachio samples after plasma streamers treatment were also investigated.

show abstract

Influence of oxygen in atmospheric-pressure argon plasma jet on sterilization of Bacillus atrophaeous spores

Cited by 94 publications

References 20 publications

Efficacy of Different Carrier Gases for Barrier Discharge Plasma Generation Compared to Chlorhexidine on the Survival of <b><i>Pseudomonas aeruginosa</i></b> Embedded in Biofilm in vitro

Efficacy of Different Carrier Gases for Barrier Discharge Plasma Generation Compared to Chlorhexidine on the Survival of <b><i>Pseudomonas aeruginosa</i></b> Embedded in Biofilm in vitro

Low temperature atmospheric pressure plasma sources for microbial decontamination

Inactivation of Aspergillus flavus spores in a sealed package by cold plasma streamers

Contact Info

Product

Resources

About