Influence of non-genetic factors on semen characteristics of Malabari bucks

Gopinathan, A.; Selvan, A. Sakthivel; Jawhar, K Thilak Pon; Karthickeyan, S.M.K.

doi:10.5958/0973-9718.2021.00004.0

Cited by 1 publication

(3 citation statements)

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“…Concerning the ejaculate order, our data demonstrated no difference between first and second ejaculate for mass activity, sperm motility and vigour. Recently, some authors reported similar results using adult bucks for sperm motility and mass activity with no difference in ejaculate order (Gopinathan et al, 2021;Morrel et al, 2022). However, we observed that the ejaculate volume and sperm concentration presented significant differences, being greater for the first ejaculate.…”

Section: Discussionmentioning

confidence: 73%

“…It is important to highlight that these related seminal parameters were obtained with a seminal analyses protocol resembling ours. Furthermore, regarding sperm motility, several studies with male adult goats showed comparable results, both obtained with subjective-related seminal analysis protocols (Shamsuddin et al, 2000;Barkawi et al, 2006;Kumbhar et al, 2019;Ebenezer et al, 2021;Gopinathan et al, 2021). Sperm quality can be evaluated as sperm characteristics before and after freezing and thawing.…”

Section: Discussionmentioning

confidence: 92%

“…Although discarding the first ejaculate is a common practice, studies point to a decrease in sperm quality after the first (Martins et al, 2006) or fourth and fifth ejaculates (Shamsuddin et al, 2000), demonstrating the inconclusiveness of this recommendation. Furthermore, some studies using adult goats observed inconsistent results regarding seminal characteristics such as mass activity and sperm concentration, volume and motility (Shamsuddin et al, 2000;Barkawi et al, 2006;Gopinathan et al, 2021;Morrell et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Seminal quality comparison of first and second cryopreserved ejaculates of Alpine kid goats by flow cytometry

Novaes

Almeida

Netto

et al. 2022

Zygote

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Summary Discarding the first ejaculate is recommended as an alternative for improving seminal quality after long sexual resting, especially when semen should be used for cryopreservation. However, when the males are not in sexual resting the necessity to discarding the first ejaculate is still unknown. Therefore, this study aimed to compare by flow cytometry the quality of the first and second ejaculates. Ten kids and uniform goats between 5 and 6 months of age were used in a completely randomized design. Semen collection was carried out every 4 days, until a total of five ejaculates per animal in each treatment was completed. The fresh and frozen semen collected were processed and analyzed using macroscopic and microscopic parameters, resistance test, hypo-osmotic medium test, and flow cytometry (FC). The FC parameters were production of reactive oxygen species, plasma and acrosomal membrane integrity, and lipid peroxidation of the plasma membrane. The ejaculates did not differ for the resistance test, the reactivity in the hypo-osmotic medium and for the macroscopic and microscopic seminal parameters, except for sperm volume and concentration. The first ejaculate had a higher percentage of minor and total defects. None of the FC parameters analyzed differed between the first and second ejaculates. The first and second ejaculates demonstrated similar seminal qualities, so for Alpine kid goats without a sexual resting period, discarding the first ejaculate it is not recommended.

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Section: Discussionmentioning

confidence: 73%