1999
DOI: 10.1590/s0036-46651999000200005 View full text |Buy / Rent full text
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Abstract: Infection of Swiss/NIH mice with Leishmania major was compared with infection in isogenic resistant C57BL/6 and susceptible BALB/c mice. Swiss/NIH mice showed self-controlled lesions in the injected foot pad. The production of high levels of interferon-gamma (IFN-gamma) and low levels of interleukin-4 (IL-4) by cells from these animals suggests that they mount a Th1-type immune response. The importance of the indigenous microbiota on the development of murine leishmaniasis was investigated by infecting germfre… Show more

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“…Antes de submeter os dados às análises de correlações canônicas no software GENES (Cruz, 2001), realizaram-se previamente testes de normalidade dos dados das variáveis (n = 25), imprescindíveis para modelos matemáticos lineares (Vieira, 1999). Foi adotado o grau de confiança de 0,01 para o teste de normalidade Shapiro-Wilk, correspondendo ao menor grau de confiança utilizado para os coeficientes das correlações canônicas.…”
Section: Methodsunclassified
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“…Antes de submeter os dados às análises de correlações canônicas no software GENES (Cruz, 2001), realizaram-se previamente testes de normalidade dos dados das variáveis (n = 25), imprescindíveis para modelos matemáticos lineares (Vieira, 1999). Foi adotado o grau de confiança de 0,01 para o teste de normalidade Shapiro-Wilk, correspondendo ao menor grau de confiança utilizado para os coeficientes das correlações canônicas.…”
Section: Methodsunclassified
“…As outras variáveis apresentaram distribuição normal dos dados originais, com probabilidades de 0,0197 a 0,9226 pelo teste ShapiroWilk. Desse modo, as duas variáveis transformadas e as demais variáveis foram submetidas às análises de variância, atendendo o pressuposto de distribuição normal para todas as variáveis (Schlotzhauer & Littell, 1997;Vieira, 1999). Para os conjuntos de 25 dados de cada uma das variáveis e sistema de uso do solo, foram calculados os intervalos de confiança da média:…”
Section: Methodsunclassified
“…As análises de variância atenderam às pressuposições básicas de distribuição normal dos resíduos pelo teste ShapiroWilk e homocedasticidade das variâncias pelo teste de Levene (Vieira, 1999), ambos com probabilidades superiores a 3 %. As análises estatísticas foram realizadas com o programa estatístico SAS (SAS, 2001).…”
Section: Methodsunclassified
“…The parasites were cultivated in NNN/Schneider medium supplemented with 20% fetal bovine serum (FBS), penicillin (1000 U/mL) and streptomycin (50 µg/mL). The cultures were incubated at 26°C until they reached stationary growth phase (18). Cytotoxicity was evaluated for 1 x 10 6 cells/mL of parasite culture in the presence of various concentrations of the venom (200, 100, 50, 25, 12.5, 6.25, 3.12 and 1.56 µg/mL) or fraction (6.25, 3.12, 1.56, 0.78 and 0.39 µg/mL).…”
Section: Leishmanicidal Effects Of B Leucurus Venom and Fractionmentioning