Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.

Opsahl-Ferstad, Hilde-Gunn; Bjørnstad, Åsmund; Rognli, Odd Arne

doi:10.1007/bf00234518

Cited by 10 publications

(3 citation statements)

References 23 publications

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“…Inbreeding of Lolium perenne through a few generations of selfing usually results in severe depression of vigour and productivity (Jenkins 1931, Nilsson 1934, Bean and Yok-Hwa 1972, Utz and Oettler 1978, but completely homozygous plants can be obtained by means of anther culture (Olesen et al 1988, Boppenmeier et al 1989, Bante et al 1990, Opsahl-Ferstad et al 1994). This method could be an attractive way of producing homozygous clones for breeding in this species, if anther-culture response among breeding material and commercial varieties of L. perenne was not so low.…”

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confidence: 99%

Inheritance of anther‐culture response in perennial ryegrass (Lolium perenne L.)

et al. 1995

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Genetic control of the capacity to respond to anther culture in perennial ryegrass was studied in F, offspring from crosses between 11 clones selected for anther-culture response ('inducers') and 10 clones selected from breeding material on the basis of good agronomic performance. Large differences in anther-culture response were observed between the two types of parent, with inducer clones producing an average of 10.7 green plants per 100 anthers, compared with only 0.3 for breeding clones. Hybrid populations produced an average of 2.2 green plants per 100 plated anthers, which is 7.3 times the response of the breeding material. This improvement was mainly due to a 9.4-fold increase in the percentage of green plants regenerated from hybrid populations to 4.7% compared to an average of 0.5% from breeding clones. Most of the GCA (general combining ability) in the experiment was contributed by the breeding material, constituting 55.8, 100.0 and 36.7% of genetic variation among hybrids for embryo formation, regeneration and green plant percentage, respectively. Any remaining genetic variation among hybrids was predominantly due to SCA (specific combining ability) effects, with percentages of 44.2 and 63.3%, respectively, for embryo and green plant formation. These results are discussed with respect to the possible exploitation of genes controlling anther-culture response for haploid production in breeding programmes of perennial ryegrass.

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confidence: 99%

Inheritance of anther‐culture response in perennial ryegrass (Lolium perenne L.)

et al. 1995

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“…When inflorescences just came out (∼1 cm) of the flag leaf in the F 2 plants, tillers were collected, cold treated for 3 to 5 d at 4°C, and surface sterilized with 70% ethanol. Anthers were isolated from the two lowest florets of each spikelet and plated on modified LS‐3 medium (Linsmaier and Skoog, 1965; Opsahl‐Ferstad et al, 1994). Anther‐derived calluses were obtained after 3 to 5 wk of culture.…”

Section: Methodsmentioning

confidence: 99%

Registration of NFLT12, a Tissue Culture–Responsive Lolium temulentum (Darnel Ryegrass) Line

Wang

Hopkins

2009

J of Plant Registrations

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Lolium temulentum L. (Darnel ryegrass) is closely related to major forage and turf species, is self‐pollinated, diploid, and has a short life cycle. These traits make it suitable to serve as a model species for testing gene functions in grasses. A new L. temulentum line, NFLT12 (Reg. No. GS‐7, PI 655941), was developed using anther culture on an F2 population derived from a cross of two parental L. temulentum lines. Haploid and doubled haploid plants were obtained and their tissue culture responsiveness and amiability to genetic transformation were tested. NFLT12 is highly responsive to tissue culture, is readily transformable and is expected to provide a resource for those using L. temulentum as a model species for functional genomics studies.

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“…Initially, only embryos and albino plants were obtained, until the first green regenerants were reported in 1984 [57]. During the following decades, contemporary protocols for barley [58] and wheat [59] AC were adapted for use in perennial ryegrass by optimizing the pre-culture temperature stress, in vitro carbohydrate source, growth regulator additions, and culture conditions, such as light and temperature [58,[60][61][62]. The total number of regenerated plants was thus increased, although the percentage of albinos remained high and genotypes capable of producing green plants by androgenesis were described as being rare exceptions [59,63].…”

Section: Doubled Haploids In Perennial Ryegrassmentioning

confidence: 99%

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

2016

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Abstract:The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium perenne L.), an important forage species, have advanced to a sufficiently successful and promising stage to merit an exploration of what their further developments may bring. The exploitation of both in vitro and in vivo haploid and DH methods to (1) purge deleterious alleles from germplasm intended for breeding; (2) develop mapping populations for genetic and genomic studies; (3) simplify haplotype mapping; (4) fix transgenes and mutations for functional gene validation and molecular breeding; and (5) hybrid cultivar development are discussed. Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands.

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Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.

Cited by 10 publications

References 23 publications

Inheritance of anther‐culture response in perennial ryegrass (Lolium perenne L.)

Inheritance of anther‐culture response in perennial ryegrass (Lolium perenne L.)

Registration of NFLT12, a Tissue Culture–Responsive Lolium temulentum (Darnel Ryegrass) Line

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

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