Influence of long-term exposure to copper on the lichen photobiont Trebouxia erici and the free-living algae Scenedesmus quadricauda

Piovár, Juraj; Stavrou, Eliza; Kaduková, Jana; Kimáková, Tatiana; Bačkor, Martin

doi:10.1007/s10725-010-9515-4

Cited by 26 publications

(14 citation statements)

References 28 publications

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“…In the accordance with the results of this study, decreases of F V /F M ratios due to Cu excess in short-term experiments using the lichen C. arbuscula subsp. mitis as well as due to chronic Cu excess in axenic cultures of lichen photobiont Asterochloris erici (Piovár et al, 2011) were previously observed. However, the presence or absence of UA in the lichen thalli had no significant effect on the degree of chlorophyll a fluorescence in thalli of C. arbuscula subsp.…”

Section: Discussionmentioning

confidence: 73%

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

Bačkorová

Biľová²,

Kimáková

et al. 2015

South African Journal of Botany

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a b s t r a c tCladina arbuscula subsp. mitis grows on the surfaces of historical Cu-mine spoil heaps rich in Cu, as well as on the surfaces of substrata that are not metal contaminated. This lichen contains the extracellular secondary metabolite, usnic acid (UA), which was previously found to form complexes with metal cations, including Cu. As UA can be experimentally removed from dry lichen thalli using acetone and without the loss of lichen viability, this study attempted to evaluate its role in long-term (8 wks) Cu tolerance in C. arbuscula subsp. mitis. Removal of UA by acetone did not affect the viability of the lichen, however it caused lower intracellular Cu accumulation after 8 wks of experiment at the highest 500 μM Cu doses tested. Thus far, we did not observe de novo synthesis of UA in the UA− variant of the study during the 8 wks of the experiment. Based on the measurements of the selected metabolic processes in the lichen (chlorophyll a fluorescence, chlorophyll a integrity, TBARS production, content of ergosterol and content of soluble proteins) it would seem that presence of UA in C. arbuscula subsp. mitis did not protect this lichen against Cu excess.

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Section: Discussionmentioning

confidence: 73%

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

Bačkorová

Biľová²,

Kimáková

et al. 2015

South African Journal of Botany

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“…Decrease of the growth can be relatively easily determined and reflects physiological status of the algal cells (Juraj et al 2011). Heavy metals had adverse effects on the growth of Scenedesmus quadricauda (Mohammed and Markert 2006; Stork et al 2013) and Spirogyra setiformis (Çelekli et al 2016) in cultures, same result also was found in this study, the inhibited of growth is mainly under high cadmium concentration, the growth of C. vulgaris decreased with the increasing cadmium concentration.…”

Section: Discussionmentioning

confidence: 99%

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

et al. 2016

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The objective of the present work was to evaluate the effect of exogenously applied cadmium on the physiological response of green algae Chlorella vulgaris. The study investigated the long-term effect (18 days) of cadmium on the levels of algae biomass, assimilation pigment composition, soluble protein, oxidative status (production of hydrogen peroxide and superoxide anion), antioxidant enzymes (such as superoxide dismutase, peroxidase, catalase and glutathione reductase enzyme) in C. vulgaris. The results showed that growth, the amount of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids gradually decreased with increasing cadmium over 18 days exposure. Cadmium at concentration of 7 mg L−1 inhibited algal growth expressed as the number of cells. Our research found that C. vulgaris has a high tolerance to cadmium. Contents of chlorophylls (Chl a and Chl b) and carotenoids (Car) of C. vulgaris was significantly decline with rising concentration of cadmium (p < 0.05). The decrease of 54.04 and 93.37 % in Chl a, 60.65 and 74.32 % in Chl b, 50.00 and 71.88 % in total carotenoids was noticed following the treatment with 3 and 7 mg L−1 cadmium doses compared with control treatment, respectively. Cadmium treatments caused a significant change in the physiological competence (calculated as chlorophyll a/b) which increased with increasing Cd(II) doses up to 1 mg L−1 but decreased at 3 mg L−1. While accumulation of soluble protein was enhanced by presence of cadmium, the treatment with cadmium at 3 and 7 mg L−1 increased the concentration of soluble proteins by 88, 95.8 % in C. vulgaris, respectively. Moreover, low doses of cadmium stimulated enzymatic (superoxide dismutase, catalase and glutathione reductase) in C. vulgaris, The content of peroxidase increased with the increasing cadmium concentration, and had slightly decreased at the concentration of 7 mg L−1, but was still higher than control group, which showed that cadmium stress at high concentration mainly peroxidase works in C. vulgaris. And therefore, suppressed reactive oxygen species (hydrogen peroxide and superoxide) accumulated. The present study also showed that cadmium increased oxidative stress and induced antioxidant defense systems against reactive oxygen species. The observation in here analyzed C. vulgaris after exposure to cadmium indicate that hydrogen peroxide, superoxide and peroxidase in the alga with exposure to Cd(II) seemed to be parameters as biomarkers for metal-induced oxidative stress.

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“…The concentration of malondialdehyde (MDA) increased with the increase in copper concentration in the nutrient solution (Monnet et al, 2005). The presence of copper negatively affected the oxidative status of the lichen photobiont (Piovár et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Copper induced physiological changes and oxidative damage in lichen Ramalina farinacea

Sujetovienė¹

2015

biologija

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Copper is an essential element for all organisms but can reach toxic concentrations as a result of human activities. The aim of the present study was to investigate physiological effects of copper on the lichen Ramalina farinacea (L.) Ach. The effect of copper on the chlorophyll content, fluorescence and oxidative status was assessed. The chlorophyll a content was not significantly different from the control. The chlorophyll b content was significantly higher at any tested Cu 2+ concentration in comparison with the control. F v /F m values decreased significantly in R. farinacea following 50 mg Cu and higher concentrations. MDA increased significantly after exposure to 100 and 500 mg Cu concentrations.

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Influence of long-term exposure to copper on the lichen photobiont Trebouxia erici and the free-living algae Scenedesmus quadricauda

Cited by 26 publications

References 28 publications

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

The presence of usnic acid does not protect the lichen Cladina arbuscula subsp. mitis against the long-term copper excess

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

Copper induced physiological changes and oxidative damage in lichen Ramalina farinacea

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