Influence of Listeria innocua on the attachment of Listeria monocytogenes to stainless steel and aluminum surfaces

Koo, Ok Kyung; Ndahetuye, Jean Baptiste; O’Bryan, Corliss A.; Ricke, Steven C.; Crandall, Philip G.

doi:10.1016/j.foodcont.2013.11.008

Cited by 14 publications

(10 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…innocua is a weaker biofilm former compared to L. monocytogenes; Koo, Ndahetuye, O'Bryan, Ricke, and Crandall (2014) used the cell enumeration method on aluminum and stainless steel and concluded that after 24 h the attachment of L. monocytogenes was significantly higher than that of L. innocua, though no significant differences were observed between both species biofilms after 72 h. In the present work, two methods were used (CV in polystyrene P96 microtiter plates and enumeration on SSC) to evaluate the biofilm forming ability of a set of L. innocua and L. monocytogenes isolates fairly representative of the contaminant microorganisms, collected from Gorgonzola processing plants. The comparison performed here, indicated that both species produced similar values, suggesting an equivalent biofilm production.…”

Section: Discussionmentioning

confidence: 99%

Listeria innocua and Listeria monocytogenes strains from dairy plants behave similarly in biofilm sanitizer testing

Costa

Lourenço

Civera

et al. 2018

LWT

View full text Add to dashboard Cite

Five L. innocua and five L. monocytogenes, including persistent and non-persistent isolates collected from Gorgonzola processing plants, were compared regarding their biofilm-forming ability and their biofilm susceptibility to two hydrogen peroxide (HP) based disinfectants in use at the plants. No significant difference in biofilm-forming ability by both species was observed (P>0.05) in crystal violet staining and viable count assays. The susceptibility to HP disinfectants of the L. monocytogenes and L. innocua biofilms was determined. In order to mimic clean and soiled biofilm forming conditions, biofilms were grown, respectively, in 1/10 diluted TSB-YE and in TSB-YE. The results showed no significant differences between species or conditions (P>0.05) regardless of whether the isolates were classified as persistent or non-persistenttb. A hierarchical clustering based on Principal Component Analysis performed on the tested variables, indicated the presence of two major clusters. Persistent and non-persistent isolates from both species were allocated in both clusters, suggesting that they behaved in a similar way in response to the tested conditions. This study showed that biofilms of in-house L. innocua could monitor the effectiveness of HP-based disinfectants. Moreover, biofilms of L. innocua could be used as surrogates of L. monocytogenes in sanitizer-based biofilm eradication trials simulating dairy processing environments, whenever the use of the pathogen is not an option.

show abstract

Section: Discussionmentioning

confidence: 99%

Listeria innocua and Listeria monocytogenes strains from dairy plants behave similarly in biofilm sanitizer testing

Costa

Lourenço

Civera

et al. 2018

LWT

View full text Add to dashboard Cite

show abstract

“…monocytogenes and their interactions which may influence whether food products become contaminated or foster the growth of Listeria, the use of continuous culture with a single DM across a range of dilution rates to study a mixture of strains and species offers some interesting avenues. Co-culture or competitive inhibition experiments have provided interesting insights into the possibility of L. innocua overgrowth of L. monocytogenes in enrichment (Koo, Ndahetuye, O"Bryan, Ricke & Crandall, 2014).…”

Section: Discussionmentioning

confidence: 99%

A review of minimal and defined media for growth of Listeria monocytogenes

et al. 2016

Self Cite

View full text Add to dashboard Cite

Listeria monocytogenes is known to be an important foodborne pathogen and is the causative agent of one of the deadliest foodborne illnesses. The organism has a wide range of environmental conditions under which it will survive and grow, and often contaminates processing plants and retail environments in which ready-to-eat foods are manufactured, prepared and served. Although L. monocytogenes is not a fastidious organism and can grow in a variety of rich media, defined and minimal media are necessary to elucidate the minimal environmental nutrients that are required for the survival and growth of this organism. In addition, many of the virulence factors required for L. monocytogenes to invade and multiply in mammalian host cells are not produced in rich media and thus induction of these factors is best studied in a minimal medium. This review covers the historical development of minimal media for Listeria spp. and explores the various factors required for survival and growth of this organism. To the best of our knowledge, this is the first time that these media have been compared side by side. In order to better compare studies using different chemical substrates such as a different carbon source, all concentrations of components in each medium have been converted to molar concentrations.

show abstract

“…The PrfA, a virulence regulator is a main factor for biofilm formation. This PrfA gene is present in all L. monocytogenes and associated with the pathogenesis of the infection [64]. Listeria monocytogenes is known to have the ability to persist for long periods on utensils and environments of food industries, thus leading to biofilm formation.…”

Section: Regulator Prfa Gene Induce Biofilm Developmentmentioning

confidence: 99%

Exploring the Pathogenesis, Clinical Characteristics and Therapeutic Regimens of Listeria monocytogenes

Loo¹,

Letchumanan²,

Dhanoa³

et al. 2020

Act Scie Micro

View full text Add to dashboard Cite

Listeria monocytogenes is a ubiquitous Gram-positive bacterium widely distributed in the soil where it lives as a saprophyte. This opportunistic pathogen causes listeriosis in humans through consumption of contaminated food especially ready-to-eat (RTE) food, chiefly deli meat, cheese, and smoked fish. Pregnant women, neonates, the elderly, and immunocompromised patients are especially vulnerable to listeriosis. The pathogenesis of listeriosis begins with ingestion of the bacterium by humans; low pH and increased gastric temperature in the gastrointestinal system upregulates the production of virulence proteins, for example PrfA and internalins, facilitating the transition of L. monocytogenes from its saprophytic existence to a pathogenic one. Internalins mediate its adherence and invasion of host intestinal epithelial cells which are usually non-phagocytic. Inside the phagosome, the low carbohydrate and low iron concentration suppress internalin production. The production of listeriolysin O (LLO) and PlcA then allows the lysis of the phagocytic vacuole, enabling the entry of bacteria into the cytosol. High levels of ActA and PlcB expression promotes cell-to-cell spread. Understanding the pathogenesis of L. monocytogenes can help appreciate the microbial virulence, treatment options, and the development of better therapeutic agents for infected patients. Therefore, this review focuses on the pathogenesis L. monocytogenes, as well as the clinical characteristics, and therapeutic regimens that would be beneficial in the management of the disease.

show abstract

Influence of Listeria innocua on the attachment of Listeria monocytogenes to stainless steel and aluminum surfaces

Cited by 14 publications

References 38 publications

Listeria innocua and Listeria monocytogenes strains from dairy plants behave similarly in biofilm sanitizer testing

Listeria innocua and Listeria monocytogenes strains from dairy plants behave similarly in biofilm sanitizer testing

A review of minimal and defined media for growth of Listeria monocytogenes

Exploring the Pathogenesis, Clinical Characteristics and Therapeutic Regimens of Listeria monocytogenes

Contact Info

Product

Resources

About