Influence of Igh-linked gene products on the generation of T helper cells in the response to sheep erythrocytes.

Abstract

The role of Igh-linked loci in the generation and expression of T cell help for antibody responses to sheep erythrocytes (SRBC) was investigated. The production of IgM, IgG3, IgG1, IgG2b, and IgG2a antibody to SRBC was shown to be T cell dependent. The Igh-congenic mouse pair CBA/Tufts (Ighj) and CBA.Ighb gave equivalent responses to SRBC. CBA.nude mice (Ighj) supplemented with peripheral T cells of either Ighj or Ighb genotype produced equivalent, high responses. Therefore, T cell-B cell mismatching for the Igh haplotype is not in itself a bar to the generation or expression of help. In contrast, T cells primed in an environment that lacks Ighj-linked products are inefficient helpers for Ighj B cells. These results suggest that antigen-primed B cells or their products prime a set of T cells that can help B cells that bear matching, Igh-linked gene products.

The IgG2a antibody response to thyroglobulin is linked to the Igh locus in mouse

“…There have been several reports suggesting that the Igh locus influences the selection of the T-cell repertoire (Nutt et al 1981;Seman and Zilberfarb 1979;Monguini and Paul 1982;B6naroch et al 1993). T cells can recognize B-cell idiotypes, especially those that are highly cross-reactive (Hetzelberger and Eichmann 1978;Gleason and K6hler 1982).…”

“…The phenomenon of isotype regulation has been described by Herzenberg and co-workers (1982). The subclass response to SRBC has been shown to be influenced by the Igh locus gene products (Nutt et al 1981), and in the C57B10 mouse may be mediated by suppressor T cells (Seman and Zilberfarb 1979). It has also been suggested that isotype switching can be influenced by cytokine production by non-T cells (Snapper and Mond 1993).…”

“…The immunoglobulin heavy chain gene locus, Igh, is of particular interest, since it has been shown to influence Correspondence to: R. Kuppers, SAIC, 5340 Spectrum Dr., Suite N, Frederick, MD 21701-7337, USA. antibody responses to several antigens (Seman and Zilberfarb 1979;Lifshitz et al 1980;Brown et al 1981;Ju et al 1981;Nutt et al 1981;Van Snick 1981;Mongini and Paul 1982;B6naroch et al 1993;Van Snick et al 1983). In this study, we report the mapping of the control of serum Tg-specific IgG2a concentrations to the Igh locus.…”

Isotype‐Specific T Cell Regulation of Immunoglobulin Expression

“…The influence of Igh-C or linked gene products on the generation of helper cells involved in allotype-or isotype-specific responses has been demonstrated in several systems. The existence of a helper cell specifically committed to help Iglb memory cells, but distinct from carrier-primed Thl, was shown by , and experiments by L'Age Stehr (1981) and Nutt et al (1981) have demonstrated Igh-Iinked restriction between B and primed T cells in responses to SRBC antigens. In the latter cases however Thl and Tig were not distinguished.…”

Correlation of the Km(1) immunoglobulin allotype with anti-polysaccharide antibodies in Caucasian adults.

“…In animals, T helper cells with specificity for allotypes, idiotypes, and isotypes have all been demonstrated (24)(25)(26). In mice, the allotype regulation ofthe antibody response to sheep erythrocytes has been demonstrated to be mediated through T cell help (27).…”